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Protein

Leucine--tRNA ligase subunit alpha

Gene

leuS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-259-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase subunit alpha (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase subunit alpha
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:aq_351
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798 Componenti: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 634634Leucine--tRNA ligase subunit alphaPRO_0000151962Add
BLAST

Interactioni

Subunit structurei

Seems to consist of an alpha chain and a beta chain.

Protein-protein interaction databases

STRINGi224324.aq_351.

Structurei

Secondary structure

1
634
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi230 – 2378Combined sources
Helixi241 – 2433Combined sources
Helixi245 – 2473Combined sources
Helixi250 – 25910Combined sources
Beta strandi260 – 26910Combined sources
Helixi271 – 2766Combined sources
Beta strandi279 – 2824Combined sources
Helixi288 – 29811Combined sources
Helixi304 – 31512Combined sources
Helixi319 – 3235Combined sources
Beta strandi330 – 3389Combined sources
Turni340 – 3423Combined sources
Beta strandi345 – 3517Combined sources
Beta strandi356 – 3583Combined sources
Beta strandi361 – 3655Combined sources
Turni367 – 3693Combined sources
Helixi371 – 38010Combined sources
Turni396 – 3983Combined sources
Helixi411 – 4133Combined sources
Helixi418 – 43114Combined sources
Beta strandi434 – 4374Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O0AX-ray1.77A/B225-443[»]
3PZ0X-ray2.40A/B/C/D228-439[»]
3PZ5X-ray2.50A/B228-439[»]
ProteinModelPortaliO66680.
SMRiO66680. Positions 2-600.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66680.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 519"HIGH" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
InParanoidiO66680.
KOiK01869.
OMAiHVKGYLA.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKEFNPREI EKKWQKRWEE AGVFKAQEGK PNKFYVLEMF PYPSGRIHMG
60 70 80 90 100
HVRNYTIGDA IARYLKMRGK NILHPMGWDA FGLPAENAAI KHGIHPAKWT
110 120 130 140 150
YENIDYMKKQ LKILGFSYDW DREIATCDPE YYKWNQWIFL KMLERGIAYR
160 170 180 190 200
KTAKVNWCPH DQTVLANEQV IEGKCWRCGT PIVQKEVPSW FLRITAYADR
210 220 230 240 250
LLEDLKKLEG KWPERVIAQQ RNWIGRSEGA LIRFYVEIEE PEKFLNCVPE
260 270 280 290 300
ELKETLLKEK RIYIDVFTTR PDTVFGATFV VLAPEHPLVP VLACIGERLG
310 320 330 340 350
NACYSDVENF VEKMKKMSTR ERTMEEDKEG VFLGVYATNP ANGEKIPVWS
360 370 380 390 400
ANYVLYEYGT GAIMCVPAHD QRDWEFAKKY DLPIKVVVKP EGAWDFEKGA
410 420 430 440 450
YEGKGTLVNS DGFDGLDSET AKRKITEWLQ DRGLGEKKVS YRLRDWNISR
460 470 480 490 500
QRYWGTPIPV VYCEKCGMVP VPEDQLPVKL PLDVKFTGQG NPLETSEEFV
510 520 530 540 550
NTTCPKCGGK ARRETDTMDT FFDSSWYFLR FCDPKNDREP FSREKVDYWM
560 570 580 590 600
PVDVYIGGIE HAVLHLLYAR FFQKFLKDLG LVRDDEPFEK LITQGMVLKK
610 620 630
WVSVKKLLDY LGLSEEDEVE ELKKRLEELG ARRA
Length:634
Mass (Da):73,989
Last modified:July 31, 1998 - v1
Checksum:i0119CA3F7A018766
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06643.1.
PIRiD70331.
RefSeqiNP_213240.1. NC_000918.1.
WP_010880178.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06643; AAC06643; aq_351.
GeneIDi1193006.
KEGGiaae:aq_351.
PATRICi20958296. VBIAquAeo85532_0284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06643.1.
PIRiD70331.
RefSeqiNP_213240.1. NC_000918.1.
WP_010880178.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O0AX-ray1.77A/B225-443[»]
3PZ0X-ray2.40A/B/C/D228-439[»]
3PZ5X-ray2.50A/B228-439[»]
ProteinModelPortaliO66680.
SMRiO66680. Positions 2-600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06643; AAC06643; aq_351.
GeneIDi1193006.
KEGGiaae:aq_351.
PATRICi20958296. VBIAquAeo85532_0284.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
InParanoidiO66680.
KOiK01869.
OMAiHVKGYLA.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-259-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO66680.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiSYLA_AQUAE
AccessioniPrimary (citable) accession number: O66680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: July 31, 1998
Last modified: March 31, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.