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O66680

- SYLA_AQUAE

UniProt

O66680 - SYLA_AQUAE

Protein

Leucine--tRNA ligase subunit alpha

Gene

leuS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-259-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase subunit alpha (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase subunit alpha
    Short name:
    LeuRS
    Gene namesi
    Name:leuS
    Ordered Locus Names:aq_351
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 634634Leucine--tRNA ligase subunit alphaPRO_0000151962Add
    BLAST

    Interactioni

    Subunit structurei

    Seems to consist of an alpha chain and a beta chain.

    Protein-protein interaction databases

    STRINGi224324.aq_351.

    Structurei

    Secondary structure

    1
    634
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi230 – 2378
    Helixi241 – 2433
    Helixi245 – 2473
    Helixi250 – 25910
    Beta strandi260 – 26910
    Helixi271 – 2766
    Beta strandi279 – 2824
    Helixi288 – 29811
    Helixi304 – 31512
    Helixi319 – 3235
    Beta strandi330 – 3389
    Turni340 – 3423
    Beta strandi345 – 3517
    Beta strandi356 – 3583
    Beta strandi361 – 3655
    Turni367 – 3693
    Helixi371 – 38010
    Turni396 – 3983
    Helixi411 – 4133
    Helixi418 – 43114
    Beta strandi434 – 4374

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3O0AX-ray1.77A/B225-443[»]
    3PZ0X-ray2.40A/B/C/D228-439[»]
    3PZ5X-ray2.50A/B228-439[»]
    ProteinModelPortaliO66680.
    SMRiO66680. Positions 2-600.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO66680.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 519"HIGH" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiVVHCDAC.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66680-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMKEFNPREI EKKWQKRWEE AGVFKAQEGK PNKFYVLEMF PYPSGRIHMG    50
    HVRNYTIGDA IARYLKMRGK NILHPMGWDA FGLPAENAAI KHGIHPAKWT 100
    YENIDYMKKQ LKILGFSYDW DREIATCDPE YYKWNQWIFL KMLERGIAYR 150
    KTAKVNWCPH DQTVLANEQV IEGKCWRCGT PIVQKEVPSW FLRITAYADR 200
    LLEDLKKLEG KWPERVIAQQ RNWIGRSEGA LIRFYVEIEE PEKFLNCVPE 250
    ELKETLLKEK RIYIDVFTTR PDTVFGATFV VLAPEHPLVP VLACIGERLG 300
    NACYSDVENF VEKMKKMSTR ERTMEEDKEG VFLGVYATNP ANGEKIPVWS 350
    ANYVLYEYGT GAIMCVPAHD QRDWEFAKKY DLPIKVVVKP EGAWDFEKGA 400
    YEGKGTLVNS DGFDGLDSET AKRKITEWLQ DRGLGEKKVS YRLRDWNISR 450
    QRYWGTPIPV VYCEKCGMVP VPEDQLPVKL PLDVKFTGQG NPLETSEEFV 500
    NTTCPKCGGK ARRETDTMDT FFDSSWYFLR FCDPKNDREP FSREKVDYWM 550
    PVDVYIGGIE HAVLHLLYAR FFQKFLKDLG LVRDDEPFEK LITQGMVLKK 600
    WVSVKKLLDY LGLSEEDEVE ELKKRLEELG ARRA 634
    Length:634
    Mass (Da):73,989
    Last modified:August 1, 1998 - v1
    Checksum:i0119CA3F7A018766
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06643.1.
    PIRiD70331.
    RefSeqiNP_213240.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC06643; AAC06643; aq_351.
    GeneIDi1193006.
    KEGGiaae:aq_351.
    PATRICi20958296. VBIAquAeo85532_0284.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06643.1 .
    PIRi D70331.
    RefSeqi NP_213240.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3O0A X-ray 1.77 A/B 225-443 [» ]
    3PZ0 X-ray 2.40 A/B/C/D 228-439 [» ]
    3PZ5 X-ray 2.50 A/B 228-439 [» ]
    ProteinModelPortali O66680.
    SMRi O66680. Positions 2-600.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_351.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC06643 ; AAC06643 ; aq_351 .
    GeneIDi 1193006.
    KEGGi aae:aq_351.
    PATRICi 20958296. VBIAquAeo85532_0284.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi VVHCDAC.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-259-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O66680.

    Family and domain databases

    Gene3Di 3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiSYLA_AQUAE
    AccessioniPrimary (citable) accession number: O66680
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3