O66662 (MURI_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate racemase
EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	aq_325

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis. Converts L- or D-glutamate to D- or L-glutamate, respectively, but not other amino acids such as alanine, aspartate, and glutamine By similarity. HAMAP MF_00258
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Subunit structure	Homodimer By similarity. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Glutamate racemase HAMAP MF_00258		PRO_0000095448

Sequences

	Sequence		Length	Mass (Da)
	O66662 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 392F6B7EC175FD87	FASTA	254	28,101
	10 20 30 40 50 60 MKIGIFDSGA GGMTVLKAIR EAYPNVDVVY LGDTARVPYG IRSKETIVRY SKECANFLKD 70 80 90 100 110 120 KGVDLLVVAC NTASAYALEE LKREFPFPVF GVIEPGVKEA LRKSKTKRIG VIGTQATIKS 130 140 150 160 170 180 GAYQEALKRA GAEVYSKACP LFVPLVEEGM IEGEIPKKVV EHYLKDFKGK VDTLILGCTH 190 200 210 220 230 240 YPLLKREIQN FLEGVNVIDS SRATAKSLKD FVKNEGSGSL ELYFTDRSQN LERLIKLILG 250 EEVEPKITSE VFVL

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06621.1.
PIR	B70329.
RefSeq	NP_213222.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66662.
SMR	O66662. Positions 1-252.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192896.
GenomeReviews	Gene locus aq_325 in contig AE000657_GR.
KEGG	aae:aq_325.
NMPDR	fig|224324.1.peg.237.
PATRIC	20958246. VBIAquAeo85532_0262.
Phylogenomic databases
HOGENOM	HBG645102.
OMA	KVEFHAS.
PhylomeDB	O66662.
ProtClustDB	CLSK2299365.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_325-MONOMER.
Family and domain databases
HAMAP	MF_00258. Glu_racemase. [Tree]
InterPro	IPR015942. Asp/Glu/hydantoin_racemase. IPR001920. Asp/Glu_race. IPR018187. Asp/Glu_racemase_AS. IPR004391. Glu_race. [Graphical view]
Gene3D	G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.
KO	K01776.
PANTHER	PTHR21198. PTHR21198. 1 hit.
Pfam	PF01177. Asp_Glu_race. 1 hit. [Graphical view]
SUPFAM	SSF53681. Asp/Glu_race. 2 hits.
TIGRFAMs	TIGR00067. Glut_race. 1 hit.
PROSITE	PS00923. ASP_GLU_RACEMASE_1. 1 hit. PS00924. ASP_GLU_RACEMASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURI_AQUAE

Accession

Primary (citable) accession number: O66662

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program