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O66651

- SYI_AQUAE

UniProt

O66651 - SYI_AQUAE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831Aminoacyl-adenylateUniRule annotation
Binding sitei627 – 6271ATPUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-227-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:aq_305
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 956956Isoleucine--tRNA ligasePRO_0000098341Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_305.

Structurei

3D structure databases

ProteinModelPortaliO66651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiO66651.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66651-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEKKVDLKD TLNLPRTEFP MKANLPQREP QILEKWKGLY EKIQKERKGR
60 70 80 90 100
EVFVLHDGPP YANGHIHVGH ALNKILKDVI NKYNLLIGKN VNFIPGWDCH
110 120 130 140 150
GLPIERAVEK ELSKKKIKKE SLPKTEFREL CREYAKKYVN IQREDFVRLG
160 170 180 190 200
VLGDWEHPYL TMDPKYEAQE IRELGKFFER GLAYRSKKPV YWCIYDKTAE
210 220 230 240 250
AEAEVEYYEK EDPSIYVKFP LKSGEKFGIK DKKVFAIIWT TTPWTLPANL
260 270 280 290 300
GIMVKEDADY VLVEVEDEVW IVAKELMDKF FETVNRPEGL VLETVKGKDL
310 320 330 340 350
VGLEYTHPFV EKEKLKGHLS EETLKNMWKI YPSEFVSLDT GTGLVHMAPG
360 370 380 390 400
HGQEDYVVGQ RYGLEPYAPV SDEGRFVEPA PEFLINVRVF DANHLIVGVL
410 420 430 440 450
KEKGFLVHEE KIRHSYPHCW RCKNPVIFRA TPQWFIGMDI EFFGKTLRQR
460 470 480 490 500
ALEEIEKVKW IPEYGKNRIK SMVENRPDWC ISRQRFWGVP ITVFYCENCG
510 520 530 540 550
EIIKDREVFE RVAQLVENSE KGSDVWFELT SSQLLPEGYK CPKCGGDSFT
560 570 580 590 600
KEEDILDVWF DSGCSHAAVI RPLGFQKADL YLEGSDQHRG WFQASLLESV
610 620 630 640 650
GSYLEAPYKA VLTHGFIVDE KGRKMSKSLG NVISPQEVVK EFGADILRLW
660 670 680 690 700
VVSEDYTEDV KLGKNLLKKI ADDYRKIRNT LRFIIGNLYD FNPRTNALPF
710 720 730 740 750
EKLHHFDRWI ISELQNLLKK VHENYEKFLF YRVHNHIKNF VITTLSAIYL
760 770 780 790 800
DVLKDRLYVY APASWERRSA QTALWHLLIA LTTSTAPYLS FTAEELWEHV
810 820 830 840 850
GKLDPSLPES VFLYEMPKPD ENLKDEEVLK DYEILLKVRD EVMRALEVAR
860 870 880 890 900
KEKGIIKHPY EAKVYIRGDE SVESLLKKYE DYLNFFFTVS QVELREGGEV
910 920 930 940 950
QIEGEELPVK VGVNKAEGEK CPRCWIYYQK EEFVGCVCKR CAKALSEMGI

ELNAVC
Length:956
Mass (Da):111,315
Last modified:August 1, 1998 - v1
Checksum:iE34E14CAB46C8AFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06614.1.
PIRiG70327.
RefSeqiNP_213211.1. NC_000918.1.
WP_010880149.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06614; AAC06614; aq_305.
GeneIDi1192885.
KEGGiaae:aq_305.
PATRICi20958220. VBIAquAeo85532_0249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06614.1 .
PIRi G70327.
RefSeqi NP_213211.1. NC_000918.1.
WP_010880149.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66651.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_305.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06614 ; AAC06614 ; aq_305 .
GeneIDi 1192885.
KEGGi aae:aq_305.
PATRICi 20958220. VBIAquAeo85532_0249.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
InParanoidi O66651.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-227-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiSYI_AQUAE
AccessioniPrimary (citable) accession number: O66651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3