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O66651

- SYI_AQUAE

UniProt

O66651 - SYI_AQUAE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei583 – 5831Aminoacyl-adenylateUniRule annotation
    Binding sitei627 – 6271ATPUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-227-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:aq_305
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 956956Isoleucine--tRNA ligasePRO_0000098341Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224324.aq_305.

    Structurei

    3D structure databases

    ProteinModelPortaliO66651.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi624 – 6285"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66651-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEKKVDLKD TLNLPRTEFP MKANLPQREP QILEKWKGLY EKIQKERKGR    50
    EVFVLHDGPP YANGHIHVGH ALNKILKDVI NKYNLLIGKN VNFIPGWDCH 100
    GLPIERAVEK ELSKKKIKKE SLPKTEFREL CREYAKKYVN IQREDFVRLG 150
    VLGDWEHPYL TMDPKYEAQE IRELGKFFER GLAYRSKKPV YWCIYDKTAE 200
    AEAEVEYYEK EDPSIYVKFP LKSGEKFGIK DKKVFAIIWT TTPWTLPANL 250
    GIMVKEDADY VLVEVEDEVW IVAKELMDKF FETVNRPEGL VLETVKGKDL 300
    VGLEYTHPFV EKEKLKGHLS EETLKNMWKI YPSEFVSLDT GTGLVHMAPG 350
    HGQEDYVVGQ RYGLEPYAPV SDEGRFVEPA PEFLINVRVF DANHLIVGVL 400
    KEKGFLVHEE KIRHSYPHCW RCKNPVIFRA TPQWFIGMDI EFFGKTLRQR 450
    ALEEIEKVKW IPEYGKNRIK SMVENRPDWC ISRQRFWGVP ITVFYCENCG 500
    EIIKDREVFE RVAQLVENSE KGSDVWFELT SSQLLPEGYK CPKCGGDSFT 550
    KEEDILDVWF DSGCSHAAVI RPLGFQKADL YLEGSDQHRG WFQASLLESV 600
    GSYLEAPYKA VLTHGFIVDE KGRKMSKSLG NVISPQEVVK EFGADILRLW 650
    VVSEDYTEDV KLGKNLLKKI ADDYRKIRNT LRFIIGNLYD FNPRTNALPF 700
    EKLHHFDRWI ISELQNLLKK VHENYEKFLF YRVHNHIKNF VITTLSAIYL 750
    DVLKDRLYVY APASWERRSA QTALWHLLIA LTTSTAPYLS FTAEELWEHV 800
    GKLDPSLPES VFLYEMPKPD ENLKDEEVLK DYEILLKVRD EVMRALEVAR 850
    KEKGIIKHPY EAKVYIRGDE SVESLLKKYE DYLNFFFTVS QVELREGGEV 900
    QIEGEELPVK VGVNKAEGEK CPRCWIYYQK EEFVGCVCKR CAKALSEMGI 950
    ELNAVC 956
    Length:956
    Mass (Da):111,315
    Last modified:August 1, 1998 - v1
    Checksum:iE34E14CAB46C8AFD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06614.1.
    PIRiG70327.
    RefSeqiNP_213211.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC06614; AAC06614; aq_305.
    GeneIDi1192885.
    KEGGiaae:aq_305.
    PATRICi20958220. VBIAquAeo85532_0249.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06614.1 .
    PIRi G70327.
    RefSeqi NP_213211.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O66651.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_305.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC06614 ; AAC06614 ; aq_305 .
    GeneIDi 1192885.
    KEGGi aae:aq_305.
    PATRICi 20958220. VBIAquAeo85532_0249.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-227-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiSYI_AQUAE
    AccessioniPrimary (citable) accession number: O66651
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3