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O66651 (SYI_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:aq_305
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 956956Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098341

Regions

Motif60 – 7011"HIGH" region HAMAP-Rule MF_02002
Motif624 – 6285"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9211Zinc By similarity
Metal binding9241Zinc By similarity
Metal binding9381Zinc By similarity
Metal binding9411Zinc By similarity
Binding site5831Aminoacyl-adenylate By similarity
Binding site6271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O66651 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E34E14CAB46C8AFD

FASTA956111,315
        10         20         30         40         50         60 
MEEKKVDLKD TLNLPRTEFP MKANLPQREP QILEKWKGLY EKIQKERKGR EVFVLHDGPP 

        70         80         90        100        110        120 
YANGHIHVGH ALNKILKDVI NKYNLLIGKN VNFIPGWDCH GLPIERAVEK ELSKKKIKKE 

       130        140        150        160        170        180 
SLPKTEFREL CREYAKKYVN IQREDFVRLG VLGDWEHPYL TMDPKYEAQE IRELGKFFER 

       190        200        210        220        230        240 
GLAYRSKKPV YWCIYDKTAE AEAEVEYYEK EDPSIYVKFP LKSGEKFGIK DKKVFAIIWT 

       250        260        270        280        290        300 
TTPWTLPANL GIMVKEDADY VLVEVEDEVW IVAKELMDKF FETVNRPEGL VLETVKGKDL 

       310        320        330        340        350        360 
VGLEYTHPFV EKEKLKGHLS EETLKNMWKI YPSEFVSLDT GTGLVHMAPG HGQEDYVVGQ 

       370        380        390        400        410        420 
RYGLEPYAPV SDEGRFVEPA PEFLINVRVF DANHLIVGVL KEKGFLVHEE KIRHSYPHCW 

       430        440        450        460        470        480 
RCKNPVIFRA TPQWFIGMDI EFFGKTLRQR ALEEIEKVKW IPEYGKNRIK SMVENRPDWC 

       490        500        510        520        530        540 
ISRQRFWGVP ITVFYCENCG EIIKDREVFE RVAQLVENSE KGSDVWFELT SSQLLPEGYK 

       550        560        570        580        590        600 
CPKCGGDSFT KEEDILDVWF DSGCSHAAVI RPLGFQKADL YLEGSDQHRG WFQASLLESV 

       610        620        630        640        650        660 
GSYLEAPYKA VLTHGFIVDE KGRKMSKSLG NVISPQEVVK EFGADILRLW VVSEDYTEDV 

       670        680        690        700        710        720 
KLGKNLLKKI ADDYRKIRNT LRFIIGNLYD FNPRTNALPF EKLHHFDRWI ISELQNLLKK 

       730        740        750        760        770        780 
VHENYEKFLF YRVHNHIKNF VITTLSAIYL DVLKDRLYVY APASWERRSA QTALWHLLIA 

       790        800        810        820        830        840 
LTTSTAPYLS FTAEELWEHV GKLDPSLPES VFLYEMPKPD ENLKDEEVLK DYEILLKVRD 

       850        860        870        880        890        900 
EVMRALEVAR KEKGIIKHPY EAKVYIRGDE SVESLLKKYE DYLNFFFTVS QVELREGGEV 

       910        920        930        940        950 
QIEGEELPVK VGVNKAEGEK CPRCWIYYQK EEFVGCVCKR CAKALSEMGI ELNAVC 

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References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC06614.1.
PIRG70327.
RefSeqNP_213211.1. NC_000918.1.

3D structure databases

ProteinModelPortalO66651.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_305.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC06614; AAC06614; aq_305.
GeneID1192885.
KEGGaae:aq_305.
PATRIC20958220. VBIAquAeo85532_0249.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-227-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_AQUAE
AccessionPrimary (citable) accession number: O66651
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries