O66640 (SSRP_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SsrA-binding protein

Gene names

Name:	smpB
Synonyms:	smb
Ordered Locus Names:	aq_287

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes. HAMAP MF_00023
Subcellular location	Cytoplasm Potential HAMAP MF_00023.
Sequence similarities	Belongs to the SmpB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 157

157

SsrA-binding protein HAMAP MF_00023

PRO_0000102894

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

157

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Turn

15 – 19

5

Beta strand

22 – 27

6

Helix

34 – 41

8

Beta strand

84 – 86

3

Helix

90 – 103

14

Beta strand

106 – 117

12

Beta strand

119 – 126

8

Sequences

	Sequence		Length	Mass (Da)
	O66640 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: FF43E3EF0FD77CFA	FASTA	157	18,530
	10 20 30 40 50 60 MGKSDKIIPI AENKEAKAKY DILETYEAGI VLKGSEVKSL REKGTVSFKD SFVRIENGEA 70 80 90 100 110 120 WLYNLYIAPY KHATIENHDP LRKRKLLLHK REIMRLYGKV QEKGYTIIPL KLYWKNNKVK 130 140 150 VLIALAKGKK LYDRRRELKE KAMKRELERE FKGKIHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
[2]	"Structure of small protein B: the protein component of the tmRNA-SmpB system for ribosome rescue." Dong G., Nowakowski J., Hoffman D.W. EMBO J. 21:1845-1854(2002) [PubMed: 11927568] [Abstract] Cited for: STRUCTURE BY NMR OF 2-134.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC06597.1.

PIR

D70326.

RefSeq

NP_213200.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K8H	NMR	-	A	2-134	[»]
1P6V	X-ray	3.20	A/C	2-157	[»]
1ZC8	electron microscopy	13.00	K	2-131	[»]
2OB7	electron microscopy	13.60	B/C	2-157	[»]

ProteinModelPortal

O66640.

SMR

O66640. Positions 2-134.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1192874.

GenomeReviews

Gene locus aq_287 in contig AE000657_GR.

KEGG

aae:aq_287.

NMPDR

fig|224324.1.peg.215.

PATRIC

20958198. VBIAquAeo85532_0238.

Phylogenomic databases

HOGENOM

HBG293422.

OMA

EKYEAGI.

PhylomeDB

O66640.

ProtClustDB

PRK05422.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_287-MONOMER.

Family and domain databases

HAMAP

MF_00023. SmpB.
[Tree]

InterPro

IPR023620. SmpB.
IPR000037. SsrA-bd_prot.
IPR020081. SsrA-bd_prot_CS.
[Graphical view]

Gene3D

G3DSA:2.40.280.10. SmpB. 1 hit.

KO

K03664.

Pfam

PF01668. SmpB. 1 hit.
[Graphical view]

ProDom

PD004488. SmpB. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF74982. SmpB. 1 hit.

TIGRFAMs

TIGR00086. SmpB. 1 hit.

PROSITE

PS01317. SSRP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SSRP_AQUAE

Accession

Primary (citable) accession number: O66640

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program