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Protein

Porphobilinogen deaminase

Gene

hemC

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.By similarity

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneBy similarityNote: Binds 1 dipyrromethane group covalently.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-197-MONOMER.
UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:hemC
Ordered Locus Names:aq_263
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Porphobilinogen deaminasePRO_0000142902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361S-(dipyrrolylmethanemethyl)cysteineBy similarity

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_263.

Structurei

3D structure databases

ProteinModelPortaliO66621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiENOG4105D6W. Bacteria.
COG0181. LUCA.
HOGENOMiHOG000228587.
InParanoidiO66621.
KOiK01749.
OMAiGIECRTD.
OrthoDBiPOG091H064B.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIRIGTRKS KLALWQANYV KDFLEKHWGV EVELVKITTT GDKITDVPLA
60 70 80 90 100
KIGGKGLFVK EIEKALLEGS IDLAVHSLKD VPMVIPKGLK LGAITKRENP
110 120 130 140 150
YDVLISRSGK KLYELPSGSV IGTSSLRRQV QIKKRRRDLK VEVLRGNVDT
160 170 180 190 200
RMRKLKEGLY DAVILAYAGV KRMGYESEIT EVLEDFIPAV GQGSLAIEIR
210 220 230 240 250
EGDKRIEELI KPLNNEESFL CAIAERTFLR RLEGGCQVPV GAFAKIENGT
260 270 280 290 300
LKMKAFISDI EAERYIEGYR EGNPEEAEKL GLSLAEELLK KGGEEILKEI

YSSQ
Length:304
Mass (Da):34,031
Last modified:December 1, 2000 - v2
Checksum:iFE9C210FA81DEE6B
GO

Sequence cautioni

The sequence AAC06580 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06580.1. Different initiation.
PIRiA70324.
RefSeqiNP_213181.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06580; AAC06580; aq_263.
GeneIDi1192855.
KEGGiaae:aq_263.
PATRICi20958160. VBIAquAeo85532_0219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06580.1. Different initiation.
PIRiA70324.
RefSeqiNP_213181.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06580; AAC06580; aq_263.
GeneIDi1192855.
KEGGiaae:aq_263.
PATRICi20958160. VBIAquAeo85532_0219.

Phylogenomic databases

eggNOGiENOG4105D6W. Bacteria.
COG0181. LUCA.
HOGENOMiHOG000228587.
InParanoidiO66621.
KOiK01749.
OMAiGIECRTD.
OrthoDBiPOG091H064B.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.
BioCyciAAEO224324:GJBH-197-MONOMER.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM3_AQUAE
AccessioniPrimary (citable) accession number: O66621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.