<?xml version='1.0' encoding='UTF-8'?>
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<entry dataset="Swiss-Prot" created="2000-05-30" modified="2013-05-01" version="88">
<accession>O66614</accession>
<name>COAD_AQUAE</name>
<protein>
<recommendedName>
<fullName>Phosphopantetheine adenylyltransferase</fullName>
<ecNumber>2.7.7.3</ecNumber>
</recommendedName>
<alternativeName>
<fullName>Dephospho-CoA pyrophosphorylase</fullName>
</alternativeName>
<alternativeName>
<fullName>Pantetheine-phosphate adenylyltransferase</fullName>
<shortName>PPAT</shortName>
</alternativeName>
</protein>
<gene>
<name type="primary">coaD</name>
<name type="synonym">kdtB</name>
<name type="ordered locus">aq_253</name>
</gene>
<organism>
<name type="scientific">Aquifex aeolicus (strain VF5)</name>
<dbReference type="NCBI Taxonomy" id="224324"/>
<lineage>
<taxon>Bacteria</taxon>
<taxon>Aquificae</taxon>
<taxon>Aquificales</taxon>
<taxon>Aquificaceae</taxon>
<taxon>Aquifex</taxon>
</lineage>
</organism>
<reference key="1">
<citation type="journal article" date="1998" name="Nature" volume="392" first="353" last="358">
<title>The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.</title>
<authorList>
<person name="Deckert G."/>
<person name="Warren P.V."/>
<person name="Gaasterland T."/>
<person name="Young W.G."/>
<person name="Lenox A.L."/>
<person name="Graham D.E."/>
<person name="Overbeek R."/>
<person name="Snead M.A."/>
<person name="Keller M."/>
<person name="Aujay M."/>
<person name="Huber R."/>
<person name="Feldman R.A."/>
<person name="Short J.M."/>
<person name="Olsen G.J."/>
<person name="Swanson R.V."/>
</authorList>
<dbReference type="PubMed" id="9537320"/>
<dbReference type="DOI" id="10.1038/32831"/>
</citation>
<scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
<source>
<strain>VF5</strain>
</source>
</reference>
<comment type="function">
<text evidence="1">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity).</text>
</comment>
<comment type="catalytic activity">
<text evidence="1">ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.</text>
</comment>
<comment type="pathway" evidence="1">
<text>Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
</comment>
<comment type="subunit">
<text>Homohexamer (By similarity).</text>
</comment>
<comment type="subcellular location">
<subcellularLocation>
<location status="by similarity">Cytoplasm</location>
</subcellularLocation>
</comment>
<comment type="similarity">
<text>Belongs to the bacterial CoaD family.</text>
</comment>
<dbReference type="EC" id="2.7.7.3"/>
<dbReference type="EMBL" id="AE000657">
<property type="protein sequence ID" value="AAC06565.1"/>
<property type="molecule type" value="Genomic_DNA"/>
</dbReference>
<dbReference type="PIR" id="B70323">
<property type="entry name" value="B70323"/>
</dbReference>
<dbReference type="RefSeq" id="NP_213174.1">
<property type="nucleotide sequence ID" value="NC_000918.1"/>
</dbReference>
<dbReference type="ProteinModelPortal" id="O66614"/>
<dbReference type="STRING" id="224324.aq_253"/>
<dbReference type="EnsemblBacteria" id="AAC06565">
<property type="protein sequence ID" value="AAC06565"/>
<property type="gene ID" value="aq_253"/>
</dbReference>
<dbReference type="GeneID" id="1192848"/>
<dbReference type="KEGG" id="aae:aq_253"/>
<dbReference type="PATRIC" id="20958132">
<property type="gene designation" value="VBIAquAeo85532_0206"/>
</dbReference>
<dbReference type="eggNOG" id="COG0669"/>
<dbReference type="HOGENOM" id="HOG000006518"/>
<dbReference type="KO" id="K00954"/>
<dbReference type="OMA" id="AMSDFEY"/>
<dbReference type="ProtClustDB" id="CLSK2748279"/>
<dbReference type="BioCyc" id="AAEO224324:GJBH-189-MONOMER"/>
<dbReference type="UniPathway" id="UPA00241">
<property type="reaction ID" value="UER00355"/>
</dbReference>
<dbReference type="GO" id="GO:0005737">
<property type="term" value="C:cytoplasm"/>
<property type="evidence" value="IEA:UniProtKB-SubCell"/>
</dbReference>
<dbReference type="GO" id="GO:0005524">
<property type="term" value="F:ATP binding"/>
<property type="evidence" value="IEA:UniProtKB-KW"/>
</dbReference>
<dbReference type="GO" id="GO:0004595">
<property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
<property type="evidence" value="IEA:HAMAP"/>
</dbReference>
<dbReference type="GO" id="GO:0015937">
<property type="term" value="P:coenzyme A biosynthetic process"/>
<property type="evidence" value="IEA:HAMAP"/>
</dbReference>
<dbReference type="Gene3D" id="3.40.50.620">
<property type="match status" value="1"/>
</dbReference>
<dbReference type="HAMAP" id="MF_00151">
<property type="entry name" value="PPAT_bact"/>
<property type="match status" value="1"/>
</dbReference>
<dbReference type="InterPro" id="IPR004821">
<property type="entry name" value="Cyt_trans-like"/>
</dbReference>
<dbReference type="InterPro" id="IPR001980">
<property type="entry name" value="LPS_biosynth"/>
</dbReference>
<dbReference type="InterPro" id="IPR014729">
<property type="entry name" value="Rossmann-like_a/b/a_fold"/>
</dbReference>
<dbReference type="Pfam" id="PF01467">
<property type="entry name" value="CTP_transf_2"/>
<property type="match status" value="1"/>
</dbReference>
<dbReference type="PRINTS" id="PR01020">
<property type="entry name" value="LPSBIOSNTHSS"/>
</dbReference>
<dbReference type="TIGRFAMs" id="TIGR01510">
<property type="entry name" value="coaD_prev_kdtB"/>
<property type="match status" value="1"/>
</dbReference>
<dbReference type="TIGRFAMs" id="TIGR00125">
<property type="entry name" value="cyt_tran_rel"/>
<property type="match status" value="1"/>
</dbReference>
<proteinExistence type="inferred from homology"/>
<keyword id="KW-0067">ATP-binding</keyword>
<keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
<keyword id="KW-0181">Complete proteome</keyword>
<keyword id="KW-0963">Cytoplasm</keyword>
<keyword id="KW-0547">Nucleotide-binding</keyword>
<keyword id="KW-0548">Nucleotidyltransferase</keyword>
<keyword id="KW-1185">Reference proteome</keyword>
<keyword id="KW-0808">Transferase</keyword>
<feature type="chain" description="Phosphopantetheine adenylyltransferase" id="PRO_0000156160" evidence="1">
<location>
<begin position="1"/>
<end position="161"/>
</location>
</feature>
<evidence key="1" type="ECO:0000203">
<source>
<dbReference type="HAMAP-Rule" id="MF_00151"/>
</source>
</evidence>
<sequence length="161" mass="18692" checksum="562700E96053BAE1" modified="1998-08-01" version="1">
MGKRVVYPGTFDPPHYGHLDIVKRSARIFDEVVVAVAKKPRKFLLFDAEERVKMFEKMVE
DIPNVEVKMFDCLLVDFMKREGINVIVRGVRLFTDFEYELQIALTNYKLAGVETVFMMPS
QEYIHISSTIVRDVASYCGDLDNMVHPYVKQKLREKFNCGS
</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
</copyright>
</uniprot>