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Protein

Phosphopantetheine adenylyltransferase

Gene

coaD

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.UniRule annotation

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes CoA from (R)-pantothenate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Type III pantothenate kinase (coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (dfp)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (dfp)
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10SubstrateUniRule annotation1
Binding sitei18ATPUniRule annotation1
Sitei18Transition state stabilizerUniRule annotation1
Binding sitei42SubstrateUniRule annotation1
Binding sitei74Substrate; via amide nitrogenUniRule annotation1
Binding sitei88SubstrateUniRule annotation1
Binding sitei99ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 11ATPUniRule annotation2
Nucleotide bindingi89 – 91ATPUniRule annotation3
Nucleotide bindingi123 – 129ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCoenzyme A biosynthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00241; UER00355.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferaseUniRule annotation (EC:2.7.7.3UniRule annotation)
Alternative name(s):
Dephospho-CoA pyrophosphorylaseUniRule annotation
Pantetheine-phosphate adenylyltransferaseUniRule annotation
Short name:
PPATUniRule annotation
Gene namesi
Name:coaDUniRule annotation
Synonyms:kdtB
Ordered Locus Names:aq_253
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001561601 – 161Phosphopantetheine adenylyltransferaseAdd BLAST161

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_253.

Structurei

3D structure databases

ProteinModelPortaliO66614.
SMRiO66614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial CoaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZEF. Bacteria.
COG0669. LUCA.
HOGENOMiHOG000006518.
InParanoidiO66614.
KOiK00954.
OMAiEFQMALM.
OrthoDBiPOG091H01F1.

Family and domain databases

CDDicd02163. PPAT. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact. 1 hit.
InterProiView protein in InterPro
IPR004821. Cyt_trans-like.
IPR001980. PPAT.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR21342:SF3. PTHR21342:SF3. 1 hit.
PfamiView protein in Pfam
PF01467. CTP_transf_like. 1 hit.
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

O66614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRVVYPGT FDPPHYGHLD IVKRSARIFD EVVVAVAKKP RKFLLFDAEE
60 70 80 90 100
RVKMFEKMVE DIPNVEVKMF DCLLVDFMKR EGINVIVRGV RLFTDFEYEL
110 120 130 140 150
QIALTNYKLA GVETVFMMPS QEYIHISSTI VRDVASYCGD LDNMVHPYVK
160
QKLREKFNCG S
Length:161
Mass (Da):18,692
Last modified:August 1, 1998 - v1
Checksum:i562700E96053BAE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06565.1.
PIRiB70323.
RefSeqiNP_213174.1. NC_000918.1.
WP_010880112.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06565; AAC06565; aq_253.
GeneIDi1192848.
KEGGiaae:aq_253.
PATRICifig|224324.8.peg.206.

Similar proteinsi

Entry informationi

Entry nameiCOAD_AQUAE
AccessioniPrimary (citable) accession number: O66614
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 27, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families