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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Charge relay systemBy similarity
Active sitei147 – 1471Charge relay systemBy similarity
Active sitei171 – 1711Acyl-ester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-185-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A (EC:6.3.5.7)
Short name:
Glu-ADT subunit A
Gene namesi
Name:gatA
Ordered Locus Names:aq_247
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000105133Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

STRINGi224324.aq_247.

Structurei

Secondary structure

1
478
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 159Combined sources
Helixi21 – 3919Combined sources
Beta strandi42 – 454Combined sources
Helixi47 – 559Combined sources
Turni63 – 664Combined sources
Beta strandi68 – 725Combined sources
Helixi87 – 893Combined sources
Helixi99 – 1068Combined sources
Beta strandi110 – 1156Combined sources
Helixi119 – 1213Combined sources
Beta strandi124 – 1263Combined sources
Beta strandi146 – 1483Combined sources
Helixi149 – 1568Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi163 – 1664Combined sources
Beta strandi168 – 1703Combined sources
Helixi173 – 1797Combined sources
Beta strandi182 – 1854Combined sources
Turni201 – 2033Combined sources
Beta strandi206 – 2127Combined sources
Helixi213 – 22311Combined sources
Helixi241 – 2444Combined sources
Beta strandi253 – 2575Combined sources
Helixi258 – 2625Combined sources
Helixi267 – 28216Combined sources
Beta strandi286 – 2905Combined sources
Helixi295 – 2973Combined sources
Helixi298 – 31316Combined sources
Turni314 – 3163Combined sources
Beta strandi318 – 3236Combined sources
Helixi332 – 34312Combined sources
Helixi346 – 35813Combined sources
Turni361 – 3677Combined sources
Helixi368 – 38518Combined sources
Turni386 – 3883Combined sources
Beta strandi390 – 3967Combined sources
Beta strandi398 – 4003Combined sources
Turni404 – 4074Combined sources
Beta strandi408 – 4103Combined sources
Helixi411 – 4155Combined sources
Helixi416 – 4183Combined sources
Turni419 – 4213Combined sources
Helixi422 – 4276Combined sources
Beta strandi431 – 4399Combined sources
Beta strandi442 – 4509Combined sources
Helixi455 – 46814Combined sources
Helixi471 – 4733Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0LX-ray2.30A/D/G/J/M/P/S/V1-478[»]
3H0MX-ray2.80A/D/G/J/M/P/S/V1-478[»]
3H0RX-ray3.00A/D/G/J/M/P/S/V1-478[»]
ProteinModelPortaliO66610.
SMRiO66610. Positions 2-476.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66610.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
InParanoidiO66610.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66610-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWKKSLSEL RELLKRGEVS PKEVVESFYD RYNQTEEKVK AYITPLYGKA
60 70 80 90 100
LKQAESLKER ELPLFGIPIA VKDNILVEGE KTTCASKILE NFVAPYDATV
110 120 130 140 150
IERLKKAGAL IVGKTNLDEF AMGSSTEYSA FFPTKNPWDL ERVPGGSSGG
160 170 180 190 200
SAASVAVLSA PVSLGSDTGG SIRQPASFCG VIGIKPTYGR VSRYGLVAFA
210 220 230 240 250
SSLDQIGVFG RRTEDVALVL EVISGWDEKD STSAKVPVPE WSEEVKKEVK
260 270 280 290 300
GLKIGLPKEF FEYELQPQVK EAFENFIKEL EKEGFEIKEV SLPHVKYSIP
310 320 330 340 350
TYYIIAPSEA SSNLARYDGV RYGYRAKEYK DIFEMYARTR DEGFGPEVKR
360 370 380 390 400
RIMLGTFALS AGYYDAYYLK AQKVRRLITN DFLKAFEEVD VIASPTTPTL
410 420 430 440 450
PFKFGERLEN PIEMYLSDIL TVPANLAGLP AISIPIAWKD GLPVGGQLIG
460 470
KHWDETTLLQ ISYLWEQKFK HYEKIPLT
Length:478
Mass (Da):53,524
Last modified:August 1, 1998 - v1
Checksum:i72EE383259D606E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06569.1.
PIRiF70322.
RefSeqiNP_213170.1. NC_000918.1.
WP_010880108.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06569; AAC06569; aq_247.
GeneIDi1192844.
KEGGiaae:aq_247.
PATRICi20958124. VBIAquAeo85532_0202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06569.1.
PIRiF70322.
RefSeqiNP_213170.1. NC_000918.1.
WP_010880108.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0LX-ray2.30A/D/G/J/M/P/S/V1-478[»]
3H0MX-ray2.80A/D/G/J/M/P/S/V1-478[»]
3H0RX-ray3.00A/D/G/J/M/P/S/V1-478[»]
ProteinModelPortaliO66610.
SMRiO66610. Positions 2-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06569; AAC06569; aq_247.
GeneIDi1192844.
KEGGiaae:aq_247.
PATRICi20958124. VBIAquAeo85532_0202.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
InParanoidiO66610.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-185-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO66610.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiGATA_AQUAE
AccessioniPrimary (citable) accession number: O66610
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.