Reviewed, UniProtKB/Swiss-Prot O66608 (PURK_AQUAE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	aq_245

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 365

365

Phosphoribosylaminoimidazole carboxylase ATPase subunit

PRO_0000074993

Regions

Domain

97 – 279

183

ATP-grasp

Nucleotide binding

168 – 171

4

ATP By similarity

Nucleotide binding

249 – 250

2

ATP By similarity

Sites

Binding site

93

1

ATP By similarity

Binding site

132

1

ATP By similarity

Binding site

143

1

ATP By similarity

Binding site

176

1

ATP By similarity

Secondary structure

1

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365

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Helix

11 – 20

10

Helix

21 – 23

3

Beta strand

26 – 30

5

Beta strand

32 – 35

4

Helix

37 – 41

5

Beta strand

42 – 46

5

Helix

48 – 50

3

Helix

51 – 57

7

Beta strand

59 – 66

8

Helix

70 – 76

7

Turn

77 – 79

3

Beta strand

80 – 82

3

Helix

85 – 90

6

Helix

93 – 101

9

Turn

102 – 104

3

Beta strand

110 – 112

3

Beta strand

129 – 132

4

Beta strand

166 – 169

4

Beta strand

174 – 183

10

Beta strand

189 – 191

3

Beta strand

194 – 200

7

Beta strand

203 – 212

10

Helix

215 – 227

13

Beta strand

232 – 240

9

Beta strand

246 – 254

9

Helix

257 – 261

5

Helix

262 – 265

4

Beta strand

266 – 268

3

Helix

270 – 278

9

Beta strand

288 – 290

3

Beta strand

292 – 299

8

Helix

302 – 304

3

Helix

307 – 310

4

Beta strand

316 – 320

5

Beta strand

330 – 337

8

Helix

341 – 353

13

Sequences

	Sequence		Length	Mass (Da)
	O66608-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C8E859448888AA42	FASTA	365	41,988
	10 20 30 40 50 60 MLTVGILGGG QLGWMTILEG RKLGFKFHVL EDKENAPACR VADRCFRTGQ ISEFVDSCDI 70 80 90 100 110 120 ITYEFEHIKD EVLEKCESKL IPNPQALYVK KSRIREKLFL KKHGFPVPEF LVIKRDEIID 130 140 150 160 170 180 ALKSFKLPVV IKAEKLGYDG KGQYRIKKLE DANQVVKNHD KEESFIIEEF VKFEAEISCI 190 200 210 220 230 240 GVRDREGKTY FYPQPFNKHE EGILIYNYVP YAKLKEAEEI TKRLMELLDI VGVFTVEFFL 250 260 270 280 290 300 LKDGRVLINE FAPRVHNTGH WTLDGAYTSQ FENLLRAITE MPLGSTELKL PSGMVNILGK 310 320 330 340 350 360 SYEEIPLKEI LSVEGAKLYW YGKEKKPRRK VGHVNVVGRS KEEVVEKVER VFTLLKGSRE KLPAP

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000657 Genomic DNA. Translation: AAC06567.1.

PIR

D70322.

RefSeq

NP_213168.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z04	X-ray	2.35	A/B	1-365	[»]

ModBase

Search...

Genome annotation databases

GeneID

1192842.

GenomeReviews

Gene locus aq_245 in contig AE000657_GR.

KEGG

aae:aq_245.

NMPDR

fig|224324.1.peg.183.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O66608.

OMA

RKIGHVN.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_245-MON.

BRENDA

4.1.1.21. 189781.

Family and domain databases

InterPro

IPR005875. AIR_COase_ATPase-su.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.

PANTHER

PTHR23047:SF1. PurK_ATP. 1 hit.

Pfam

PF02222. ATP-grasp. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01161. purK. 1 hit.

PROSITE

PS50975. ATP_GRASP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PURK_AQUAE

Accession

Primary (citable) accession number: O66608

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)