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Protein

Lon protease

Gene

lon

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei692UniRule annotation1
Active sitei735UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi370 – 377ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiS16.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon proteaseUniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease LaUniRule annotation
Gene namesi
Name:lonUniRule annotation
Ordered Locus Names:aq_242
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000761141 – 795Lon proteaseAdd BLAST795

Proteomic databases

PRIDEiO66605.

Expressioni

Inductioni

By heat shock.UniRule annotation

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224324.aq_242.

Structurei

3D structure databases

ProteinModelPortaliO66605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 212Lon N-terminalPROSITE-ProRule annotationAdd BLAST196
Domaini605 – 787Lon proteolyticPROSITE-ProRule annotationAdd BLAST183

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
InParanoidiO66605.
KOiK01338.
OMAiIRTYLTW.
OrthoDBiPOG091H00WA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNELFQTPQV EAGIKEYPLM PLRDIVIFPT MVQPLFVGRR FSIRAIEEAN
60 70 80 90 100
KKDKLIFLVL QKDKDVEEPK EEDIYKVGVV AYILRTVPIE DARVKVLVQG
110 120 130 140 150
LKRGVIKKLE WKEDHYVAQV DVIEERDIPP ESQTIEDKAL IKAVKESIDK
160 170 180 190 200
LVSLGKQIIP DLVVLIKELE EPGKLADMVA SILDIKSSQA QEILETFDPR
210 220 230 240 250
ERLKKVYKFL QDEIGLLEVK QRISEIARER MEKEQREYYL RQQLKAIQEE
260 270 280 290 300
LGEAGGIKAE IEEYTKKFEE VKECMPEEGV KEVEKNIKRL ERLHPESAEA
310 320 330 340 350
GVIRTWLDWV LDLPWCTRTE DNYDLERARE ILDRDHYDLE KVKDRIIEYL
360 370 380 390 400
AIRKLTQGKE APTQILAFVG PPGVGKTSLG RSIAEALGRK FVRIALGGIR
410 420 430 440 450
DEAEIRGHRR TYVGAMPGRI IQAIKQAGTK NPVIMLDEID KLAISFQGDP
460 470 480 490 500
AAALLEVLDP EQNKKFTDLY IGIPFDLSEV IFICTGNRAD TIPTPLLDRM
510 520 530 540 550
ELIMLSGYSE EEKLFIAKKH LIPKLIPLHG FSPEEIEFTD EAILEIIRGY
560 570 580 590 600
TREAGVRNLQ RQISAVLRKI AVKKLQGEKG PFNITPELVR KLLGVPRYRP
610 620 630 640 650
EREKKPLVGV ATGLAWTEVG GEIMFIEATK MKGKGSLVLT GSLGDIMKES
660 670 680 690 700
AQAALSYIRS KAEDYGIDPD IFSQVDVHVH VPEGAVPKDG PSAGVAIATA
710 720 730 740 750
LLSLFTDIPV RMDVAMTGEI TLRGRVLPVG GLKEKILAAK RAEIYEVILP
760 770 780 790
AKNKDEVMEE LPEYVREKMT LHFVDNLEEV FKIALVREPK PLKEA
Length:795
Mass (Da):89,972
Last modified:August 1, 1998 - v1
Checksum:iEC8FDBB7F24EFEFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06568.1.
PIRiA70322.
RefSeqiNP_213165.1. NC_000918.1.
WP_010880103.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06568; AAC06568; aq_242.
GeneIDi1192839.
KEGGiaae:aq_242.
PATRICi20958114. VBIAquAeo85532_0197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06568.1.
PIRiA70322.
RefSeqiNP_213165.1. NC_000918.1.
WP_010880103.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_242.

Protein family/group databases

MEROPSiS16.001.

Proteomic databases

PRIDEiO66605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06568; AAC06568; aq_242.
GeneIDi1192839.
KEGGiaae:aq_242.
PATRICi20958114. VBIAquAeo85532_0197.

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
InParanoidiO66605.
KOiK01338.
OMAiIRTYLTW.
OrthoDBiPOG091H00WA.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLON_AQUAE
AccessioniPrimary (citable) accession number: O66605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.