O66605 (LON_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	aq_242

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	Lon protease		PRO_0000076114
Regions
	Domain	16 – 211	196	Lon
	Nucleotide binding	370 – 377	8	ATP By similarity
Sites
	Active site	692	1	By similarity
	Active site	735	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66605 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: EC8FDBB7F24EFEFB	FASTA	795	89,972
	10 20 30 40 50 60 MNELFQTPQV EAGIKEYPLM PLRDIVIFPT MVQPLFVGRR FSIRAIEEAN KKDKLIFLVL 70 80 90 100 110 120 QKDKDVEEPK EEDIYKVGVV AYILRTVPIE DARVKVLVQG LKRGVIKKLE WKEDHYVAQV 130 140 150 160 170 180 DVIEERDIPP ESQTIEDKAL IKAVKESIDK LVSLGKQIIP DLVVLIKELE EPGKLADMVA 190 200 210 220 230 240 SILDIKSSQA QEILETFDPR ERLKKVYKFL QDEIGLLEVK QRISEIARER MEKEQREYYL 250 260 270 280 290 300 RQQLKAIQEE LGEAGGIKAE IEEYTKKFEE VKECMPEEGV KEVEKNIKRL ERLHPESAEA 310 320 330 340 350 360 GVIRTWLDWV LDLPWCTRTE DNYDLERARE ILDRDHYDLE KVKDRIIEYL AIRKLTQGKE 370 380 390 400 410 420 APTQILAFVG PPGVGKTSLG RSIAEALGRK FVRIALGGIR DEAEIRGHRR TYVGAMPGRI 430 440 450 460 470 480 IQAIKQAGTK NPVIMLDEID KLAISFQGDP AAALLEVLDP EQNKKFTDLY IGIPFDLSEV 490 500 510 520 530 540 IFICTGNRAD TIPTPLLDRM ELIMLSGYSE EEKLFIAKKH LIPKLIPLHG FSPEEIEFTD 550 560 570 580 590 600 EAILEIIRGY TREAGVRNLQ RQISAVLRKI AVKKLQGEKG PFNITPELVR KLLGVPRYRP 610 620 630 640 650 660 EREKKPLVGV ATGLAWTEVG GEIMFIEATK MKGKGSLVLT GSLGDIMKES AQAALSYIRS 670 680 690 700 710 720 KAEDYGIDPD IFSQVDVHVH VPEGAVPKDG PSAGVAIATA LLSLFTDIPV RMDVAMTGEI 730 740 750 760 770 780 TLRGRVLPVG GLKEKILAAK RAEIYEVILP AKNKDEVMEE LPEYVREKMT LHFVDNLEEV 790 FKIALVREPK PLKEA

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06568.1.
PIR	A70322.
RefSeq	NP_213165.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66605.
SMR	O66605. Positions 608-789.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192839.
GenomeReviews	Gene locus aq_242 in contig AE000657_GR.
KEGG	aae:aq_242.
NMPDR	fig|224324.1.peg.180.
PATRIC	20958114. VBIAquAeo85532_0197.
Phylogenomic databases
HOGENOM	HBG566281.
OMA	KDAGTIN.
PhylomeDB	O66605.
ProtClustDB	CLSK2299336.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_242-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_AQUAE

Accession

Primary (citable) accession number: O66605

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program