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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821NucleophileBy similarity
Active sitei169 – 1691By similarity
Active sitei171 – 1711By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi223 – 2297ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-176-MONOMER.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Ordered Locus Names:aq_236
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510GMP synthase [glutamine-hydrolyzing]PRO_0000140088Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_236.

Structurei

3D structure databases

ProteinModelPortaliO66601.
SMRiO66601. Positions 1-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 195191Glutamine amidotransferase type-1Add
BLAST
Domaini196 – 385190GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
InParanoidiO66601.
KOiK01951.
OMAiMSHGDSV.
OrthoDBiEOG6JHRJV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRRPILVVN FGSQYVQLIA RRVRELGVYS EIVHWDTPVE EIKKKNPYGI
60 70 80 90 100
IFSGGPASVY AEGAPLPDKR IYELGVPILG ICYGLQVITH QLGGKVVRSE
110 120 130 140 150
KQEYGRARLR IIKEDVIFEG IPKESDVWMS HADKVVELPE GFEVLAVSEN
160 170 180 190 200
SPYAVIANRE KKIYGFQFHP EVTHTVFGKE MLANFIYGVC KAEKNWEMGD
210 220 230 240 250
FIHEKIEEIR KTVGDAKVIA ALSGGVDSTV AAVLTHRAIG DKLHCFFIDH
260 270 280 290 300
GLLRYKEREE VEKNLRSLGL PLTVVDASEE FLEKLKGVED PEEKRKIIGR
310 320 330 340 350
TFIEVFEREA KKIEGAEFLL QGTLYPDVVE SAGIKGSAKI KTHHNVGGLP
360 370 380 390 400
ERMNLKLLEP FRELFKDEVR KIGKLLGVPE EILRRHPFPG PGLAIRIIGE
410 420 430 440 450
VNKKDLEILR KADYIFIQEL KKEGLYDRVW QAFAVLLPVK SVGVMGDVRT
460 470 480 490 500
YEKVVALRAV ESVDGMTADW ARLPYDFLDR VMRRIINEVE GVNRVVYDIS
510
SKPPSTIEWE
Length:510
Mass (Da):57,858
Last modified:August 1, 1998 - v1
Checksum:i2855029D68D1A667
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06558.1.
PIRiE70321.
RefSeqiNP_213161.1. NC_000918.1.
WP_010880099.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06558; AAC06558; aq_236.
GeneIDi1192835.
KEGGiaae:aq_236.
PATRICi20958106. VBIAquAeo85532_0193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06558.1.
PIRiE70321.
RefSeqiNP_213161.1. NC_000918.1.
WP_010880099.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66601.
SMRiO66601. Positions 1-510.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_236.

Protein family/group databases

MEROPSiC26.957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06558; AAC06558; aq_236.
GeneIDi1192835.
KEGGiaae:aq_236.
PATRICi20958106. VBIAquAeo85532_0193.

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
InParanoidiO66601.
KOiK01951.
OMAiMSHGDSV.
OrthoDBiEOG6JHRJV.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BioCyciAAEO224324:GJBH-176-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiGUAA_AQUAE
AccessioniPrimary (citable) accession number: O66601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.