O66601 (GUAA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	aq_236

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW asparagine biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000140088
Regions
	Domain	5 – 195	191	Glutamine amidotransferase type-1
	Domain	196 – 385	190	GMPS ATP-PPase
	Nucleotide binding	223 – 229	7	ATP By similarity
Sites
	Active site	82	1	Nucleophile By similarity
	Active site	169	1	By similarity
	Active site	171	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66601 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2855029D68D1A667	FASTA	510	57,858
	10 20 30 40 50 60 MQRRPILVVN FGSQYVQLIA RRVRELGVYS EIVHWDTPVE EIKKKNPYGI IFSGGPASVY 70 80 90 100 110 120 AEGAPLPDKR IYELGVPILG ICYGLQVITH QLGGKVVRSE KQEYGRARLR IIKEDVIFEG 130 140 150 160 170 180 IPKESDVWMS HADKVVELPE GFEVLAVSEN SPYAVIANRE KKIYGFQFHP EVTHTVFGKE 190 200 210 220 230 240 MLANFIYGVC KAEKNWEMGD FIHEKIEEIR KTVGDAKVIA ALSGGVDSTV AAVLTHRAIG 250 260 270 280 290 300 DKLHCFFIDH GLLRYKEREE VEKNLRSLGL PLTVVDASEE FLEKLKGVED PEEKRKIIGR 310 320 330 340 350 360 TFIEVFEREA KKIEGAEFLL QGTLYPDVVE SAGIKGSAKI KTHHNVGGLP ERMNLKLLEP 370 380 390 400 410 420 FRELFKDEVR KIGKLLGVPE EILRRHPFPG PGLAIRIIGE VNKKDLEILR KADYIFIQEL 430 440 450 460 470 480 KKEGLYDRVW QAFAVLLPVK SVGVMGDVRT YEKVVALRAV ESVDGMTADW ARLPYDFLDR 490 500 510 VMRRIINEVE GVNRVVYDIS SKPPSTIEWE

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06558.1.
PIR	E70321.
RefSeq	NP_213161.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66601.
SMR	O66601. Positions 1-510.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192835.
GenomeReviews	Gene locus aq_236 in contig AE000657_GR.
KEGG	aae:aq_236.
NMPDR	fig|224324.1.peg.176.
PATRIC	20958106. VBIAquAeo85532_0193.
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
PhylomeDB	O66601.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_236-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR001962. Asn_synthase. IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00733. Asn_synthase. 1 hit. PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_AQUAE

Accession

Primary (citable) accession number: O66601

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program