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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871MagnesiumUniRule annotation
Metal bindingi493 – 4931MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-168-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:aq_221
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 775775Polyribonucleotide nucleotidyltransferasePRO_0000329502Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_221.

Structurei

3D structure databases

ProteinModelPortaliO66593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHUniRule annotationAdd
BLAST
Domaini623 – 69371S1 motif 1UniRule annotationAdd
BLAST
Domaini707 – 77569S1 motif 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 2 S1 motif domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
InParanoidiO66593.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 2 hits.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 2 hits.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 2 hits.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEEKARIG NSEEPIIIET GKYAKLTDGS VVVRQGGTAV LVTAVMSDEP
60 70 80 90 100
ITDVDFTPLA VDYRERASAY GRIPGGFTKR EGKPTDREIL VSRVIDRPIR
110 120 130 140 150
PLFPEGFFHD VIITALTLSA DDKYDPDVLA ITGASAALHI SRIPFEGPIA
160 170 180 190 200
GVRVCRVNGE FVANPTYEQR KEADLDIVMA GTKDAIVMVE GGGKEIPEEV
210 220 230 240 250
LADALFFGLD AIKEVIEAQE RLREKVGKPK FEYQKVELPE DILKALEEEC
260 270 280 290 300
TPKILEAFNI KDKKERYSTL DKIVEEFIEA HQIPEELHFA VKYFYKKLES
310 320 330 340 350
RLMREKVLKE GVRIDGRKPN EIRPIWIEVH PFERPHGNAI FTRGQTQAYV
360 370 380 390 400
TVTLGTPDEA LIIETIAEGE VFKRFMLHYS MPPFSVGEAK PWGPPRRREI
410 420 430 440 450
GHGALAERAI EPLLPPEEEY PFIIRVVSDI LESNGSTSMA TVCGASLALF
460 470 480 490 500
DAGVPMKDNK HVAGIAMGLI LEKDRYVILS DILGDEDHLG DMDFKVAGTK
510 520 530 540 550
DGITSVQMDI KVKGITKEIM LDALKQAREG RLYILEKMYE AIPEPRKEPH
560 570 580 590 600
PYTPKVEVVD VPEEKAPLII GPGGSTVKKI YDETGVKVWV GEQGKVYLFV
610 620 630 640 650
FPGGDVEKAK QMIQDIVREV EVGAVYKGTI TRVEPYGVFV ELWPGKIGLL
660 670 680 690 700
HVSKMAEPVR SATEKYKVGE EIIVKVLDLD ELGRPRFTTI GIEDVGTEKK
710 720 730 740 750
EVKPKVGDVY EGKVVRVEPY GAFIEYAPGK VGLLHVSKMK ERVKDARQKY
760 770
KVGDVVKVKV VEIDEQGRPK FTDDV
Length:775
Mass (Da):86,489
Last modified:August 1, 1998 - v1
Checksum:i391F3B28E2AF3E67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06562.1.
PIRiE70320.
RefSeqiNP_213153.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06562; AAC06562; aq_221.
GeneIDi1192827.
KEGGiaae:aq_221.
PATRICi20958088. VBIAquAeo85532_0184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06562.1.
PIRiE70320.
RefSeqiNP_213153.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_221.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06562; AAC06562; aq_221.
GeneIDi1192827.
KEGGiaae:aq_221.
PATRICi20958088. VBIAquAeo85532_0184.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
InParanoidiO66593.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-168-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 2 hits.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 2 hits.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 2 hits.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPNP_AQUAE
AccessioniPrimary (citable) accession number: O66593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 1, 1998
Last modified: March 4, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.