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O66593 (PNP_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:aq_221
OrganismAquifex aeolicus (strain VF5)
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 2 S1 motif domains.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRepeat
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 775775Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000329502

Regions

Domain554 – 61360KH
Domain623 – 69371S1 motif 1
Domain707 – 77569S1 motif 2

Sequences

Sequence LengthMass (Da)Tools
O66593 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 391F3B28E2AF3E67

FASTA77586,489
        10         20         30         40         50         60 
MSVEEKARIG NSEEPIIIET GKYAKLTDGS VVVRQGGTAV LVTAVMSDEP ITDVDFTPLA 

        70         80         90        100        110        120 
VDYRERASAY GRIPGGFTKR EGKPTDREIL VSRVIDRPIR PLFPEGFFHD VIITALTLSA 

       130        140        150        160        170        180 
DDKYDPDVLA ITGASAALHI SRIPFEGPIA GVRVCRVNGE FVANPTYEQR KEADLDIVMA 

       190        200        210        220        230        240 
GTKDAIVMVE GGGKEIPEEV LADALFFGLD AIKEVIEAQE RLREKVGKPK FEYQKVELPE 

       250        260        270        280        290        300 
DILKALEEEC TPKILEAFNI KDKKERYSTL DKIVEEFIEA HQIPEELHFA VKYFYKKLES 

       310        320        330        340        350        360 
RLMREKVLKE GVRIDGRKPN EIRPIWIEVH PFERPHGNAI FTRGQTQAYV TVTLGTPDEA 

       370        380        390        400        410        420 
LIIETIAEGE VFKRFMLHYS MPPFSVGEAK PWGPPRRREI GHGALAERAI EPLLPPEEEY 

       430        440        450        460        470        480 
PFIIRVVSDI LESNGSTSMA TVCGASLALF DAGVPMKDNK HVAGIAMGLI LEKDRYVILS 

       490        500        510        520        530        540 
DILGDEDHLG DMDFKVAGTK DGITSVQMDI KVKGITKEIM LDALKQAREG RLYILEKMYE 

       550        560        570        580        590        600 
AIPEPRKEPH PYTPKVEVVD VPEEKAPLII GPGGSTVKKI YDETGVKVWV GEQGKVYLFV 

       610        620        630        640        650        660 
FPGGDVEKAK QMIQDIVREV EVGAVYKGTI TRVEPYGVFV ELWPGKIGLL HVSKMAEPVR 

       670        680        690        700        710        720 
SATEKYKVGE EIIVKVLDLD ELGRPRFTTI GIEDVGTEKK EVKPKVGDVY EGKVVRVEPY 

       730        740        750        760        770 
GAFIEYAPGK VGLLHVSKMK ERVKDARQKY KVGDVVKVKV VEIDEQGRPK FTDDV

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References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC06562.1.
PIRE70320.
RefSeqNP_213153.1. NC_000918.1.

3D structure databases

HSSPHSSP built from PDB template 1SRO based on UniProtKB P05055.
ProteinModelPortalO66593.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1192827.
GenomeReviewsGene locus aq_221 in contig AE000657_GR.
KEGGaae:aq_221.
NMPDRfig|224324.1.peg.168.
PATRIC20958088. VBIAquAeo85532_0184.

Phylogenomic databases

HOGENOMHBG382411.
OMAYGETVVL.
PhylomeDBO66593.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycAAEO224324:AQ_221-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 2 hits.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 2 hits.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 2 hits.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF50249. Nucleic_acid_OB. 2 hits.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_AQUAE
AccessionPrimary (citable) accession number: O66593
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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