O66587 (CYNS_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyanate hydratase
Short name=Cyanase
EC=4.2.1.104

Alternative name(s):
Cyanate hydrolase
Cyanate lyase

Gene names

Name:	cynS
Ordered Locus Names:	aq_212

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide By similarity. HAMAP MF_00535
Catalytic activity	Cyanate + HCO3- + 2 H+ = NH3 + 2 CO2. HAMAP MF_00535
Sequence similarities	Belongs to the cyanase family.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cyanate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cyanate hydratase activity Inferred from electronic annotation. Source: EC hydro-lyase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Cyanate hydratase HAMAP MF_00535		PRO_0000187521
Sites
	Active site	90	1	By similarity
	Active site	93	1	By similarity
	Active site	116	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66587 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 0E6F5F4FCF340A5C	FASTA	149	17,201
	10 20 30 40 50 60 MRSDIGRLSK YLIERKKNLG LTWEDVSRKL GKSPVYCAML FYGYAQADDE EVKAVAELLN 70 80 90 100 110 120 LEEKELAELK DAPYREPQQP VPPTDPFVYR LYEVVILYGP ALKDVAHEMF GDGIMSAIDM 130 140 SVELEKVEQE GAERMVLTFN GKWLKYRKF

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06548.1.
PIR	G70319.
RefSeq	NP_213147.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66587.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192821.
GenomeReviews	Gene locus aq_212 in contig AE000657_GR.
KEGG	aae:aq_212.
NMPDR	fig|224324.1.peg.162.
PATRIC	20958076. VBIAquAeo85532_0178.
Phylogenomic databases
HOGENOM	HBG317643.
OMA	QTIPLRG.
ProtClustDB	PRK02866.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_212-MONOMER.
Family and domain databases
HAMAP	MF_00535. Cyanate_hydrat. [Tree]
InterPro	IPR008076. Cyanase. IPR003712. Cyanate_lyase_C. IPR001387. HTH_3. IPR010982. Lambda_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.1160.10. Cyanate_lyase_C. 1 hit. G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.
KO	K01725.
Pfam	PF02560. Cyanate_lyase. 1 hit. PF01381. HTH_3. 1 hit. [Graphical view]
PIRSF	PIRSF001263. Cyanate_hydratas. 1 hit.
PRINTS	PR01693. CYANASE.
SMART	SM00530. HTH_XRE. 1 hit. [Graphical view]
SUPFAM	SSF55234. Cyanate_lyase_C. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
TIGRFAMs	TIGR00673. CynS. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYNS_AQUAE

Accession

Primary (citable) accession number: O66587

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program