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Protein

Ribosomal RNA small subunit methyltransferase E

Gene

rsmE

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase E (EC:2.1.1.193)
Alternative name(s):
16S rRNA m3U1498 methyltransferase
Gene namesi
Name:rsmE
Ordered Locus Names:aq_165
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001762041 – 229Ribosomal RNA small subunit methyltransferase EAdd BLAST229

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_165.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi13 – 17Combined sources5
Helixi19 – 26Combined sources8
Beta strandi35 – 39Combined sources5
Beta strandi42 – 51Combined sources10
Beta strandi53 – 63Combined sources11
Beta strandi70 – 78Combined sources9
Helixi84 – 96Combined sources13
Beta strandi100 – 105Combined sources6
Helixi113 – 134Combined sources22
Helixi148 – 150Combined sources3
Beta strandi155 – 161Combined sources7
Beta strandi163 – 165Combined sources3
Helixi170 – 172Combined sources3
Beta strandi178 – 184Combined sources7
Helixi192 – 200Combined sources9
Beta strandi204 – 206Combined sources3
Beta strandi209 – 211Combined sources3
Helixi215 – 228Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EGVX-ray1.45A/B1-229[»]
2EGWX-ray2.80A/B1-229[»]
ProteinModelPortaliO66552.
SMRiO66552.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66552.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA methyltransferase RsmE family.Curated

Phylogenomic databases

eggNOGiENOG4107XZW. Bacteria.
COG1385. LUCA.
HOGENOMiHOG000154206.
InParanoidiO66552.
KOiK09761.
OMAiPNILRAN.
OrthoDBiPOG091H02MI.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR006700. rRNA_ssu_MeTrfase-E.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04452. Methyltrans_RNA. 1 hit.
[Graphical view]
PIRSFiPIRSF015601. MTase_slr0722. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00046. TIGR00046. 1 hit.

Sequencei

Sequence statusi: Complete.

O66552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVFYSEERR GNLLILREGE VKHFRVRRIE KDEEFGVIHE GKIYVCKVRR
60 70 80 90 100
EDKREISCEI VEELETKLPP KDITLYQSVT VDLKTMDTIV RQATELGVLT
110 120 130 140 150
FVPIISERSF QKEEAILKKT EKWKRIVIEA MKQSRRPIPM EIKKPVRLSD
160 170 180 190 200
LIPESEENII LDNFYEGVKP KDVNLEAKTY SVVVGPEGGF SKRESQILRE
210 220
KGFKSVLLEP YTLRTETAVV SIVSILMNF
Length:229
Mass (Da):26,683
Last modified:August 1, 1998 - v1
Checksum:i5E83FB8432B5B500
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06516.1.
PIRiE70315.
RefSeqiNP_213112.1. NC_000918.1.
WP_010880050.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06516; AAC06516; aq_165.
GeneIDi1192695.
KEGGiaae:aq_165.
PATRICi20958002. VBIAquAeo85532_0141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06516.1.
PIRiE70315.
RefSeqiNP_213112.1. NC_000918.1.
WP_010880050.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EGVX-ray1.45A/B1-229[»]
2EGWX-ray2.80A/B1-229[»]
ProteinModelPortaliO66552.
SMRiO66552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_165.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06516; AAC06516; aq_165.
GeneIDi1192695.
KEGGiaae:aq_165.
PATRICi20958002. VBIAquAeo85532_0141.

Phylogenomic databases

eggNOGiENOG4107XZW. Bacteria.
COG1385. LUCA.
HOGENOMiHOG000154206.
InParanoidiO66552.
KOiK09761.
OMAiPNILRAN.
OrthoDBiPOG091H02MI.

Miscellaneous databases

EvolutionaryTraceiO66552.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR006700. rRNA_ssu_MeTrfase-E.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04452. Methyltrans_RNA. 1 hit.
[Graphical view]
PIRSFiPIRSF015601. MTase_slr0722. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00046. TIGR00046. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSME_AQUAE
AccessioniPrimary (citable) accession number: O66552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.