Reviewed, UniProtKB/Swiss-Prot O66519 (PGK_AQUAE)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	aq_118

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Phosphoglycerate kinase HAMAP MF_00145		PRO_0000145898
Regions
	Nucleotide binding	351 – 354	4	ATP By similarity
	Region	22 – 24	3	Substrate binding By similarity
	Region	61 – 64	4	Substrate binding By similarity
Sites
	Binding site	38	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	203	1	ATP By similarity
	Binding site	294	1	ATP; via carbonyl oxygen By similarity
	Binding site	325	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66519-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: BF66BA208A8EBC57	FASTA	397	43,956
	10 20 30 40 50 60 MLRKKTLRDV DVKGKRVLVR VDYNVPLDEQ GNIVDDTRIR ASLPTVEYLL DANAKVILMS 70 80 90 100 110 120 HLGRPKNRDP KYSLAPVAKR LSRYINKEVK LAPDCVGEEV KRIVNSMKEG DVVLLENLRF 130 140 150 160 170 180 HKEETECDEN FARELASLGE VYVADAFGTC HRKHASVYLV PKFLKPAVMG FLLEKEITYF 190 200 210 220 230 240 EKAMVAPQRP VVAILGGAKV SSKLEVIKNL IRRVDKLFIG GAMAFTFLKA MGYKVGNSLV 250 260 270 280 290 300 EDDLQDVARD LIDVAKKLEI KLYLPVDFVI GQEVSENTPT KVVPWQEIPD GWMGLDIGPV 310 320 330 340 350 360 SVELVKEIIS DAQTIVWNGP MGVFEIDKFK HGTIEVAKLL AQSSALTIAG GGDTDHAIHK 370 380 390 AGVYHAFDFV STGGGAFLEL LAGKELPCLV NLDDKEA

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06475.1.
PIR	D70311.
RefSeq	NP_213079.1.
3D structure databases
HSSP	HSSP built from PDB template 1VPE based on UniProtKB P36204.
ModBase	Search...
Genome annotation databases
GeneID	1192530.
GenomeReviews	Gene locus aq_118 in contig AE000657_GR.
KEGG	aae:aq_118.
NMPDR	fig|224324.1.peg.94.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66519.
OMA	CLENTRF.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_118-MON.
BRENDA	2.7.2.3. 189781.
Family and domain databases
HAMAP	MF_00145. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_AQUAE

Accession

Primary (citable) accession number: O66519

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)