Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine synthetase

Gene

glnA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi133Magnesium 1By similarity1
Metal bindingi135Magnesium 2By similarity1
Binding sitei207ATPBy similarity1
Metal bindingi212Magnesium 2By similarity1
Metal bindingi220Magnesium 2By similarity1
Binding sitei265L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi269Magnesium 1; via pros nitrogenBy similarity1
Binding sitei273ATPBy similarity1
Binding sitei321L-glutamateBy similarity1
Binding sitei327L-glutamateBy similarity1
Binding sitei339ATPBy similarity1
Binding sitei339L-glutamateBy similarity1
Binding sitei344ATPBy similarity1
Binding sitei353ATPBy similarity1
Metal bindingi358Magnesium 1By similarity1
Binding sitei360L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 273ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:aq_111
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532281 – 469Glutamine synthetaseAdd BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei398O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi224324.aq_111.

Structurei

3D structure databases

ProteinModelPortaliO66514.
SMRiO66514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 265L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
InParanoidiO66514.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.

Sequencei

Sequence statusi: Complete.

O66514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKYTPEEVL DLIQKEGVQY VDLRFSDPFG QWQHLTIPAY EISKETFEVG
60 70 80 90 100
RGFDGSSIRG WQSINESDML AKPDPNTAFI DPFIEPKTLV MICDIYDPVT
110 120 130 140 150
GERYGRDTRY IAQKAEQYLK QTGIGDTAYF GPEAEFFIFD SVEFGTAANY
160 170 180 190 200
AFWRVDSEEG WWNREVPSSG YKIPHKRGYF PAPPVDKMMQ LRNEMVSIMS
210 220 230 240 250
DLGIIVELHH HEVATAGQGE IDIRYDSLLN QADKLFLYKY IVRMVAAKHG
260 270 280 290 300
KYATFMAKVL PNDNGSGMHT HFSIWKNGEN LFAGSEYAGL SKTALYAIGG
310 320 330 340 350
ILKHGPAIAA FTNPTVNSYH RLVPGYEAPV RLAYSARNRS AAIRIPMYSQ
360 370 380 390 400
NPKAKRIEVR FPDATSNPYL AFAAILMAAI DGIENEIDPG EPFDKDIYSL
410 420 430 440 450
PPEELEGIPQ LPGSLEEALK ALEEDYEFLL KGNVFTEEFI QLWIESKRAE
460
IDELRFIPHP KEFELYWDI
Length:469
Mass (Da):53,294
Last modified:August 1, 1998 - v1
Checksum:i688504C9FD95B056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06472.1.
PIRiG70310.
RefSeqiNP_213074.1. NC_000918.1.
WP_010880012.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06472; AAC06472; aq_111.
GeneIDi1192525.
KEGGiaae:aq_111.
PATRICi20957910. VBIAquAeo85532_0095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06472.1.
PIRiG70310.
RefSeqiNP_213074.1. NC_000918.1.
WP_010880012.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66514.
SMRiO66514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06472; AAC06472; aq_111.
GeneIDi1192525.
KEGGiaae:aq_111.
PATRICi20957910. VBIAquAeo85532_0095.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
InParanoidiO66514.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_AQUAE
AccessioniPrimary (citable) accession number: O66514
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: March 15, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.