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Protein

Nitrogen regulatory protein P-II

Gene

glnB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-88-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II
Gene namesi
Name:glnB
Ordered Locus Names:aq_109
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 112112Nitrogen regulatory protein P-IIPRO_0000139768Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511O-UMP-tyrosinePROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_109.

Structurei

Secondary structure

1
112
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi10 – 123Combined sources
Helixi13 – 2210Combined sources
Beta strandi29 – 357Combined sources
Beta strandi56 – 6510Combined sources
Helixi67 – 693Combined sources
Helixi70 – 8112Combined sources
Beta strandi90 – 956Combined sources
Beta strandi97 – 1015Combined sources
Turni102 – 1043Combined sources
Helixi108 – 1103Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EG1X-ray1.80A1-112[»]
2EG2X-ray1.72A1-112[»]
2Z0GX-ray2.10A/B/C/D1-112[»]
ProteinModelPortaliO66513.
SMRiO66513. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66513.

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiO66513.
KOiK04751.
OMAiKIMLEIV.
OrthoDBiPOG091H03RA.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIEAIIKP FKLDEVKDAL VEIGIGGMTV TEVKGFGQQK GHTEIYRGTE
60 70 80 90 100
YVIDFLPKVK IEVVVRDEDV EKVVETIVKT AQTGRVGDGK IFIIPVEDVI
110
RIRTGERGEQ AI
Length:112
Mass (Da):12,497
Last modified:August 1, 1998 - v1
Checksum:i0E44B4B171A6233B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06473.1.
PIRiF70310.
RefSeqiNP_213073.1. NC_000918.1.
WP_010880011.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06473; AAC06473; aq_109.
GeneIDi1192524.
KEGGiaae:aq_109.
PATRICi20957908. VBIAquAeo85532_0094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06473.1.
PIRiF70310.
RefSeqiNP_213073.1. NC_000918.1.
WP_010880011.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EG1X-ray1.80A1-112[»]
2EG2X-ray1.72A1-112[»]
2Z0GX-ray2.10A/B/C/D1-112[»]
ProteinModelPortaliO66513.
SMRiO66513. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06473; AAC06473; aq_109.
GeneIDi1192524.
KEGGiaae:aq_109.
PATRICi20957908. VBIAquAeo85532_0094.

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
InParanoidiO66513.
KOiK04751.
OMAiKIMLEIV.
OrthoDBiPOG091H03RA.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-88-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO66513.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNB_AQUAE
AccessioniPrimary (citable) accession number: O66513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.