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O66503

- RIR1_AQUAE

UniProt

O66503 - RIR1_AQUAE

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Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdA, aq_094
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the alpha subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71Allosteric activator By similarity
Binding sitei52 – 521Allosteric activator By similarity
Binding sitei88 – 881Allosteric activator By similarity
Binding sitei219 – 2191Substrate By similarity
Sitei235 – 2351Important for hydrogen atom transfer By similarity
Sitei242 – 2421Allosteric effector binding By similarity
Binding sitei263 – 2631Substrate; via amide nitrogen By similarity
Sitei272 – 2721Allosteric effector binding By similarity
Active sitei483 – 4831Proton acceptor By similarity
Active sitei485 – 4851Cysteine radical intermediate By similarity
Active sitei487 – 4871Proton acceptor By similarity
Sitei521 – 5211Important for hydrogen atom transfer By similarity
Sitei783 – 7831Important for electron transfer By similarity
Sitei784 – 7841Important for electron transfer By similarity
Sitei796 – 7961Interacts with thioredoxin/glutaredoxin By similarity
Sitei799 – 7991Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-77-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:aq_094
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 801801Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi235 ↔ 521Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi224324.aq_094.

Structurei

3D structure databases

ProteinModelPortaliO66503.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 9290ATP-cone
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 197Allosteric activator binding By similarity
Regioni234 – 2352Substrate binding By similarity
Regioni483 – 4875Substrate binding By similarity
Regioni680 – 6845Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000057034.
KOiK00525.
OMAiEACLMCQ.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66503-1 [UniParc]FASTAAdd to Basket

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MTMYVIKRSG RKEKLDINKI RIAIKFACEG LNVDPLELEA DAQIQFRDGI    50
TTKEIQQLLI KTAAEKVSAE RPDWTYTAAR LLLYDLYKDV AHLRGYSLRD 100
DLGKYKPYNR KNFYSFVKEY VEKGIYGEYL LENYSEEDFN KLANYIKPER 150
DLYFTYTGIK ILYDRYLVRD EEGRVIELPQ EMYMLIAMTL AVPEKPEERL 200
KWAKKFYDVL SEHKVTVATP TLMNARRPFT QLSSCFVLTV DDDLFDIFDN 250
VKKAGMISKF AGGLGVYLGK IRATGAPIRK FKGASSGVIP VVKLINDTMT 300
YVDQLGMRKG SASITLDIWH KDILDFLEVK TNVGDERKKA HDIHPAVSIP 350
DLFMKRLKNR EDWTLIDPYW ARQYITRKIY DGKYKEVKPL PGSHYYVGIK 400
EDGTQDILEP KGLEDFYGEE FEKWYLELEE NLPSYAKKKV NSFELWKRLL 450
TVAFETGEPY IFFRDEANRK NPNKHTGMVY SSNLCHEIVQ TMSPSKHEKP 500
VLDPETGEIT YKKEAGDLPV CNLGSVNLGK VHTEEEIKEV LPLLVRMLDN 550
VIEMNFYAIP EAEYTNKRYR AIGIGVSNYH YCLVKNGIKW ESEEHLKFAD 600
KLFELIAFYA LKGSLELAKE RGRYKLFDGS NWSKGILFGR SVEEIEENSR 650
QNGNNLPWRE LAEEIKKYGI RNAYLLALMP TGSTSLILGA TPSIDPIFAR 700
FYKEENMSGI LPQVPPEVDR FYWHYKTAYT IDHEWTIRAA AVRQKWIDQA 750
QSLNLFVDPQ NIDGPRLSRL YELAWELGLK TIYYLRSRSA MDIEECEACS 800
V 801
Length:801
Mass (Da):92,914
Last modified:August 1, 1998 - v1
Checksum:iFF728EDC7D97C396
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06460.1.
PIRiD70309.
RefSeqiNP_213062.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06460; AAC06460; aq_094.
GeneIDi1192514.
KEGGiaae:aq_094.
PATRICi20957884. VBIAquAeo85532_0082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06460.1 .
PIRi D70309.
RefSeqi NP_213062.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66503.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_094.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06460 ; AAC06460 ; aq_094 .
GeneIDi 1192514.
KEGGi aae:aq_094.
PATRICi 20957884. VBIAquAeo85532_0082.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000057034.
KOi K00525.
OMAi EACLMCQ.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci AAEO224324:GJBH-77-MONOMER.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiRIR1_AQUAE
AccessioniPrimary (citable) accession number: O66503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3