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Reviewed, UniProtKB/Swiss-Prot O66496 (KDSA_AQUAE)

Last modified February 9, 2010. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS
Gene names
Name: kdsA
Ordered Locus Names: aq_085
OrganismAquifex aeolicus [Complete proteome] [HAMAP]
Taxonomic identifier63363 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

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Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056

Pathway

Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056

Subunit structure

Oligomer. HAMAP MF_00056

Subcellular location

Cytoplasm By similarity HAMAP MF_00056.

Sequence similarities

Belongs to the kdsA family.

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Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processketo-3-deoxy-D-manno-octulosonic acid biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3-deoxy-8-phosphooctulonate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2672672-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056
PRO_0000187099

Secondary structure

1 ............................................ 267
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
O66496-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 0A3CFEA3337F1964

FASTA26729,734
        10         20         30         40         50         60 
MEKFLVIAGP CAIESEELLL KVGEEIKRLS EKFKEVEFVF KSSFDKANRS SIHSFRGHGL 

        70         80         90        100        110        120 
EYGVKALRKV KEEFGLKITT DIHESWQAEP VAEVADIIQI PAFLCRQTDL LLAAAKTGRA 

       130        140        150        160        170        180 
VNVKKGQFLA PWDTKNVVEK LKFGGAKEIY LTERGTTFGY NNLVVDFRSL PIMKQWAKVI 

       190        200        210        220        230        240 
YDATHSVQLP GGLGDKSGGM REFIFPLIRA AVAVGCDGVF METHPEPEKA LSDASTQLPL 

       250        260 
SQLEGIIEAI LEIREVASKY YETIPVK

« Hide

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References

« Hide 'large scale' references
[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
[2]"Functional and biochemical characterization of a recombinant 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus."
Duewel H.S., Sheflyan G.Y., Woodard R.W.
Biochem. Biophys. Res. Commun. 263:346-351(1999) [PubMed: 10491295] [Abstract]
Cited for: CHARACTERIZATION.
[3]"Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase."
Wang J., Duewel H.S., Stuckey J.A., Woodard R.W., Gatti D.L.
J. Mol. Biol. 324:205-214(2002) [PubMed: 12441100] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC06457.1.
PIRE70308.
RefSeqNP_213056.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FWNX-ray1.94A/B1-267[»]
1FWSX-ray1.90A/B1-267[»]
1FWTX-ray1.90A/B1-267[»]
1FWWX-ray1.85A/B1-267[»]
1FX6X-ray2.06A/B1-267[»]
1FXPX-ray1.80A/B1-267[»]
1FXQX-ray1.80A/B1-267[»]
1FY6X-ray1.89A/B1-267[»]
1JCXX-ray1.80A/B1-267[»]
1JCYX-ray1.90A/B1-267[»]
1LRNX-ray2.10A/B1-267[»]
1LROX-ray1.80A/B1-267[»]
1LRQX-ray1.80A/B1-267[»]
1PCKX-ray1.80A/B1-267[»]
1PCWX-ray1.85A/B1-267[»]
1PE1X-ray1.74A/B1-267[»]
1T8XX-ray1.80A/B1-267[»]
1T96X-ray1.85A/B1-267[»]
1T99X-ray1.85A/B1-267[»]
1ZHAX-ray1.74A/B1-267[»]
1ZJIX-ray2.25A/B1-267[»]
2A21X-ray1.80A/B1-267[»]
2A2IX-ray1.95A/B1-267[»]
2EF9X-ray2.00A/B1-267[»]
2NWRX-ray1.50A/B1-267[»]
2NWSX-ray1.80A/B1-267[»]
2NX1X-ray1.80A/B1-267[»]
2NX3X-ray2.10A/B/C/D/E/F/G/H/I/J/K/L1-267[»]
2NXGX-ray1.95A/B2-264[»]
2NXHX-ray2.11A/B/C/D/E/F/G/H/I/J/K/L2-264[»]
2NXIX-ray2.30A/B/C/D/E/F/G/H/I/J/K/L2-264[»]
3E0IX-ray1.70A/B1-267[»]
3E12X-ray1.70A/B1-267[»]
ModBaseSearch...

Genome annotation databases

GeneID1192509.
GenomeReviewsGene locus aq_085 in contig AE000657_GR.
KEGGaae:aq_085.
NMPDRfig|224324.1.peg.71.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG575990.
OMAVHEPHQA.
PhylomeDBO66496.

Enzyme and pathway databases

BioCycAAEO224324:AQ_085-MONOMER.
BRENDA2.5.1.55. 189781.

Family and domain databases

HAMAPMF_00056. KDO8P_synth.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006269. KDO8P_synthase.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR21057:SF2. KDO8P_synthase. 1 hit.
PfamPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01362. KDO8P_synth. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameKDSA_AQUAE
AccessionPrimary (citable) accession number: O66496
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents