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Protein

Chorismate synthase

Gene

aroC

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441NADPUniRule annotation
Binding sitei50 – 501NADPUniRule annotation
Binding sitei306 – 3061FMN; via amide nitrogenUniRule annotation
Binding sitei347 – 3471FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1353FMNUniRule annotation
Nucleotide bindingi261 – 2622FMNUniRule annotation
Nucleotide bindingi321 – 3255FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-68-MONOMER.
BRENDAi4.2.3.5. 396.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:aq_081
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Chorismate synthasePRO_0000140537Add
BLAST

Proteomic databases

PRIDEiO66493.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224324.aq_081.

Structurei

Secondary structure

1
398
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Beta strandi16 – 249Combined sources
Helixi34 – 4512Combined sources
Beta strandi61 – 688Combined sources
Beta strandi77 – 826Combined sources
Turni119 – 1235Combined sources
Helixi128 – 1347Combined sources
Helixi136 – 1383Combined sources
Helixi139 – 15416Combined sources
Turni155 – 1573Combined sources
Beta strandi159 – 1679Combined sources
Helixi173 – 1753Combined sources
Helixi178 – 1825Combined sources
Helixi184 – 19411Combined sources
Helixi204 – 2063Combined sources
Helixi207 – 21812Combined sources
Turni219 – 2213Combined sources
Beta strandi226 – 2349Combined sources
Beta strandi242 – 2443Combined sources
Helixi245 – 2473Combined sources
Helixi249 – 25810Combined sources
Beta strandi263 – 2686Combined sources
Helixi271 – 2766Combined sources
Helixi279 – 2813Combined sources
Beta strandi286 – 2894Combined sources
Turni290 – 2923Combined sources
Beta strandi293 – 2975Combined sources
Turni300 – 3034Combined sources
Beta strandi314 – 3207Combined sources
Helixi353 – 37624Combined sources
Helixi381 – 39616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q1LX-ray2.05A/B/C/D1-398[»]
DisProtiDP00559.
ProteinModelPortaliO66493.
SMRiO66493. Positions 2-398.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66493.

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
InParanoidiO66493.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRYLRFLT AGESHGKGLT AILEGIPANL PLSEEEINHE LRRRQRGYGR
60 70 80 90 100
GGRMKIEKDT AEILSGVRFG KTLGSPIALF IRNRDWENWK EKMAIEGEPS
110 120 130 140 150
PSVVPFTRPR PGHADLSGGI KYNQRDLRNI LERASARETA ARVAVGAVCK
160 170 180 190 200
KFLSEFGIKI GSFVVSIGQK EVEELKDKSY FANPEKLLSY HEKAEDSELR
210 220 230 240 250
IPFPEKDEEF KTYIDEVKEK GESLGGVFEV FALNVPPGLG SHIQWDRRID
260 270 280 290 300
GRIAQAMMSI QAIKGVEIGL GFEAARRFGS QVHDEIGWSE GKGYFRHSNN
310 320 330 340 350
LGGTEGGITN GMPIVVRVAM KPIPTLKNPL RSVDIETKEE MKAGKERTDI
360 370 380 390
VAVPAASVVG EAMLAIVLAD ALLEKLGGDF MEEVKKRFED YVNHVKSF
Length:398
Mass (Da):44,254
Last modified:August 1, 1998 - v1
Checksum:iA0C59327BFA3B39A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06434.1.
PIRiB70308.
RefSeqiNP_213053.1. NC_000918.1.
WP_010879991.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06434; AAC06434; aq_081.
GeneIDi1192506.
KEGGiaae:aq_081.
PATRICi20957861. VBIAquAeo85532_0071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06434.1.
PIRiB70308.
RefSeqiNP_213053.1. NC_000918.1.
WP_010879991.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q1LX-ray2.05A/B/C/D1-398[»]
DisProtiDP00559.
ProteinModelPortaliO66493.
SMRiO66493. Positions 2-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_081.

Proteomic databases

PRIDEiO66493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06434; AAC06434; aq_081.
GeneIDi1192506.
KEGGiaae:aq_081.
PATRICi20957861. VBIAquAeo85532_0071.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
InParanoidiO66493.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciAAEO224324:GJBH-68-MONOMER.
BRENDAi4.2.3.5. 396.

Miscellaneous databases

EvolutionaryTraceiO66493.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "Crystal structure of chorismate synthase from Aquifex aeolicus reveals a novel beta alpha beta sandwich topology."
    Viola C.M., Saridakis V., Christendat D.
    Proteins 54:166-169(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiAROC_AQUAE
AccessioniPrimary (citable) accession number: O66493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.