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Protein

Protein translocase subunit SecY

Gene

secY

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-66-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecY
Gene namesi
Name:secY
Ordered Locus Names:aq_079
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicAdd
BLAST
Transmembranei14 – 4027Helical; Name=1Add
BLAST
Topological domaini41 – 6424PeriplasmicAdd
BLAST
Transmembranei65 – 9834Discontinuously helical; Name=Helix 2Add
BLAST
Intramembranei65 – 728Helical; Name=Helix 2A
Intramembranei73 – 775
Intramembranei78 – 9821Helical; Name=Helix 2BAdd
BLAST
Topological domaini99 – 11113CytoplasmicAdd
BLAST
Transmembranei112 – 13221Helical; Name=3Add
BLAST
Topological domaini133 – 15422PeriplasmicAdd
BLAST
Transmembranei155 – 17824Helical; Name=4Add
BLAST
Topological domaini179 – 1813Cytoplasmic
Transmembranei182 – 20019Helical; Name=5Add
BLAST
Topological domaini201 – 21414PeriplasmicAdd
BLAST
Transmembranei215 – 23521Helical; Name=6Add
BLAST
Topological domaini236 – 26732CytoplasmicAdd
BLAST
Transmembranei268 – 28619Helical; Name=7Add
BLAST
Topological domaini287 – 30721PeriplasmicAdd
BLAST
Transmembranei308 – 32922Helical; Name=8Add
BLAST
Topological domaini330 – 36031CytoplasmicAdd
BLAST
Transmembranei361 – 38525Helical; Name=9Add
BLAST
Topological domaini386 – 3938Periplasmic
Transmembranei394 – 41421Helical; Name=10Add
BLAST
Topological domaini415 – 42915CytoplasmicAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Protein translocase subunit SecYPRO_0000131707Add
BLAST

Interactioni

Subunit structurei

Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-59810N.
STRINGi224324.aq_079.

Structurei

Secondary structure

1
429
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 2914
Beta strandi58 – 614
Helixi78 – 9316
Beta strandi97 – 993
Helixi110 – 12819
Helixi132 – 1354
Helixi154 – 16310
Helixi167 – 17812
Helixi186 – 1905
Turni194 – 2007
Helixi216 – 22914
Helixi274 – 29017
Turni299 – 3024
Helixi309 – 32719
Helixi332 – 3387
Helixi355 – 36915
Helixi373 – 38513
Helixi399 – 4035
Helixi407 – 41711

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DL8X-ray7.50G/H1-429[»]
ProteinModelPortaliO66491.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66491.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CGG. Bacteria.
COG0201. LUCA.
HOGENOMiHOG000080586.
InParanoidiO66491.
KOiK03076.
OMAiQTYVISQ.
OrthoDBiEOG651SWP.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
HAMAPiMF_01465. SecY.
InterProiIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYLKALFE LKELRQKFIF TLLMFVIYRL GSHIPIPGIN PEALRDFLKA
60 70 80 90 100
FEGSVFALYD IFSGGNLGRL TVFALGVMPY ISASIMMQLL TVAIPSLQRL
110 120 130 140 150
AKEEGDYGRY KINEYTKYLT LFVATVQSLG IAFWIRGQVS PKGIPVVENP
160 170 180 190 200
GISFILITVL TLVAGTMFLV WIADRITEKG IGNGASLIIF AGIVANFPNA
210 220 230 240 250
VIQFYEKVKT GDIGPLTLLL IIALIIAIIV GIVYVQEAER RIPIQYPGRQ
260 270 280 290 300
VGRQLYAGRK TYLPIKINPA GVIPIIFAQA LLLIPSTLLN FVQNPFIKVI
310 320 330 340 350
ADMFQPGAIF YNFLYVTFIV FFTYFYTAVL INPVELAENL HKAGAFIPGV
360 370 380 390 400
RPGQDTVKYL ERIINRLIFF GALFLSVIAL IPILISVWFN IPFYFGGTTA
410 420
LIVVGVALDT FRQIETYLIQ KKYKSYVRR
Length:429
Mass (Da):48,100
Last modified:August 1, 1998 - v1
Checksum:i87D4FC133D037C25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06435.1.
PIRiH70307.
RefSeqiNP_213051.1. NC_000918.1.
WP_010879989.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06435; AAC06435; aq_079.
GeneIDi1192504.
KEGGiaae:aq_079.
PATRICi20957857. VBIAquAeo85532_0069.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06435.1.
PIRiH70307.
RefSeqiNP_213051.1. NC_000918.1.
WP_010879989.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DL8X-ray7.50G/H1-429[»]
ProteinModelPortaliO66491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59810N.
STRINGi224324.aq_079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06435; AAC06435; aq_079.
GeneIDi1192504.
KEGGiaae:aq_079.
PATRICi20957857. VBIAquAeo85532_0069.

Phylogenomic databases

eggNOGiENOG4105CGG. Bacteria.
COG0201. LUCA.
HOGENOMiHOG000080586.
InParanoidiO66491.
KOiK03076.
OMAiQTYVISQ.
OrthoDBiEOG651SWP.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-66-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO66491.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
HAMAPiMF_01465. SecY.
InterProiIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "Structure of a complex of the ATPase SecA and the protein-translocation channel."
    Zimmer J., Nam Y., Rapoport T.A.
    Nature 455:936-943(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (7.5 ANGSTROMS) OF SECYEG IN COMPLEX WITH B.SUBTILIS SECA.

Entry informationi

Entry nameiSECY_AQUAE
AccessioniPrimary (citable) accession number: O66491
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.