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O66490

- KAD_AQUAE

UniProt

O66490 - KAD_AQUAE

Protein

Adenylate kinase

Gene

adk

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.UniRule annotation

    Catalytic activityi

    ATP + AMP = 2 ADP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311AMP
    Binding sitei89 – 891AMP
    Binding sitei120 – 1201ATP
    Binding sitei124 – 1241ATP
    Binding sitei161 – 1611AMP
    Binding sitei189 – 1891ATP; via carbonyl oxygen

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156ATP
    Nucleotide bindingi57 – 593AMP
    Nucleotide bindingi82 – 854AMP
    Nucleotide bindingi133 – 1342ATP

    GO - Molecular functioni

    1. adenylate kinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP salvage Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-65-MONOMER.
    UniPathwayiUPA00588; UER00649.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate kinaseUniRule annotation (EC:2.7.4.3UniRule annotation)
    Short name:
    AKUniRule annotation
    Alternative name(s):
    ATP-AMP transphosphorylaseUniRule annotation
    ATP:AMP phosphotransferaseUniRule annotation
    Adenylate monophosphate kinaseUniRule annotation
    Gene namesi
    Name:adkUniRule annotation
    Ordered Locus Names:aq_078
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 206206Adenylate kinasePRO_0000158718Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224324.aq_078.

    Structurei

    Secondary structure

    1
    206
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi13 – 2412
    Beta strandi27 – 304
    Helixi31 – 4111
    Helixi44 – 5512
    Helixi61 – 7111
    Beta strandi74 – 763
    Beta strandi78 – 825
    Helixi87 – 9913
    Beta strandi106 – 1116
    Helixi114 – 1229
    Beta strandi124 – 1263
    Turni128 – 1303
    Beta strandi133 – 1353
    Turni136 – 1383
    Helixi151 – 1533
    Helixi155 – 16814
    Helixi171 – 1777
    Turni178 – 1803
    Beta strandi182 – 1865
    Helixi191 – 20212

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2RGXX-ray1.90A1-206[»]
    2RH5X-ray2.48A/B/C1-206[»]
    3SR0X-ray1.56A/B1-206[»]
    ProteinModelPortaliO66490.
    SMRiO66490. Positions 1-203.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO66490.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni30 – 5930NMPbindAdd
    BLAST
    Regioni123 – 15331LIDAdd
    BLAST

    Domaini

    Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.

    Sequence similaritiesi

    Belongs to the adenylate kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0563.
    HOGENOMiHOG000238772.
    KOiK00939.
    OMAiVLNIEVP.
    OrthoDBiEOG679TH4.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00235. Adenylate_kinase_Adk.
    InterProiIPR006259. Adenyl_kin_sub.
    IPR000850. Adenylat/UMP-CMP_kin.
    IPR007862. Adenylate_kinase_lid-dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR23359. PTHR23359. 1 hit.
    PRINTSiPR00094. ADENYLTKNASE.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF57774. SSF57774. 1 hit.
    TIGRFAMsiTIGR01351. adk. 1 hit.
    PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66490-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILVFLGPPG AGKGTQAKRL AKEKGFVHIS TGDILREAVQ KGTPLGKKAK    50
    EYMERGELVP DDLIIALIEE VFPKHGNVIF DGFPRTVKQA EALDEMLEKK 100
    GLKVDHVLLF EVPDEVVIER LSGRRINPET GEVYHVKYNP PPPGVKVIQR 150
    EDDKPEVIKK RLEVYREQTA PLIEYYKKKG ILRIIDASKP VEEVYRQVLE 200
    VIGDGN 206
    Length:206
    Mass (Da):23,231
    Last modified:August 1, 1998 - v1
    Checksum:i2310B49B11CBC71E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06438.1.
    PIRiG70307.
    RefSeqiNP_213050.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC06438; AAC06438; aq_078.
    GeneIDi1192503.
    KEGGiaae:aq_078.
    PATRICi20957855. VBIAquAeo85532_0068.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06438.1 .
    PIRi G70307.
    RefSeqi NP_213050.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2RGX X-ray 1.90 A 1-206 [» ]
    2RH5 X-ray 2.48 A/B/C 1-206 [» ]
    3SR0 X-ray 1.56 A/B 1-206 [» ]
    ProteinModelPortali O66490.
    SMRi O66490. Positions 1-203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_078.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC06438 ; AAC06438 ; aq_078 .
    GeneIDi 1192503.
    KEGGi aae:aq_078.
    PATRICi 20957855. VBIAquAeo85532_0068.

    Phylogenomic databases

    eggNOGi COG0563.
    HOGENOMi HOG000238772.
    KOi K00939.
    OMAi VLNIEVP.
    OrthoDBi EOG679TH4.

    Enzyme and pathway databases

    UniPathwayi UPA00588 ; UER00649 .
    BioCyci AAEO224324:GJBH-65-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O66490.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00235. Adenylate_kinase_Adk.
    InterProi IPR006259. Adenyl_kin_sub.
    IPR000850. Adenylat/UMP-CMP_kin.
    IPR007862. Adenylate_kinase_lid-dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR23359. PTHR23359. 1 hit.
    PRINTSi PR00094. ADENYLTKNASE.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF57774. SSF57774. 1 hit.
    TIGRFAMsi TIGR01351. adk. 1 hit.
    PROSITEi PS00113. ADENYLATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH BI-SUBSTRATE ANALOG AP5A.

    Entry informationi

    Entry nameiKAD_AQUAE
    AccessioniPrimary (citable) accession number: O66490
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3