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O66490

- KAD_AQUAE

UniProt

O66490 - KAD_AQUAE

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Protein

Adenylate kinase

Gene

adk

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311AMP
Binding sitei89 – 891AMP
Binding sitei120 – 1201ATP
Binding sitei124 – 1241ATP
Binding sitei161 – 1611AMP
Binding sitei189 – 1891ATP; via carbonyl oxygen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156ATP
Nucleotide bindingi57 – 593AMP
Nucleotide bindingi82 – 854AMP
Nucleotide bindingi133 – 1342ATP

GO - Molecular functioni

  1. adenylate kinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. AMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-65-MONOMER.
UniPathwayiUPA00588; UER00649.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinaseUniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AKUniRule annotation
Alternative name(s):
ATP-AMP transphosphorylaseUniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
Gene namesi
Name:adkUniRule annotation
Ordered Locus Names:aq_078
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Adenylate kinasePRO_0000158718Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_078.

Structurei

Secondary structure

1
206
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi13 – 2412Combined sources
Beta strandi27 – 304Combined sources
Helixi31 – 4111Combined sources
Helixi44 – 5512Combined sources
Helixi61 – 7111Combined sources
Beta strandi74 – 763Combined sources
Beta strandi78 – 825Combined sources
Helixi87 – 9913Combined sources
Beta strandi106 – 1116Combined sources
Helixi114 – 1229Combined sources
Beta strandi124 – 1263Combined sources
Turni128 – 1303Combined sources
Beta strandi133 – 1353Combined sources
Turni136 – 1383Combined sources
Helixi151 – 1533Combined sources
Helixi155 – 16814Combined sources
Helixi171 – 1777Combined sources
Turni178 – 1803Combined sources
Beta strandi182 – 1865Combined sources
Helixi191 – 20212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RGXX-ray1.90A1-206[»]
2RH5X-ray2.48A/B/C1-206[»]
3SR0X-ray1.56A/B1-206[»]
4IKEX-ray1.48A/B1-206[»]
4JKYX-ray2.37A/B1-203[»]
4JL5X-ray1.24A/B1-203[»]
4JL6X-ray1.65A/B1-203[»]
4JL8X-ray1.79A/B1-203[»]
4JLAX-ray2.12A/B1-203[»]
4JLBX-ray1.53A/B1-203[»]
4JLDX-ray1.55A/B1-203[»]
4JLOX-ray1.73A/B1-203[»]
4JLPX-ray1.43A/B1-203[»]
ProteinModelPortaliO66490.
SMRiO66490. Positions 1-203.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66490.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 5930NMPbindAdd
BLAST
Regioni123 – 15331LIDAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.

Sequence similaritiesi

Belongs to the adenylate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0563.
HOGENOMiHOG000238772.
InParanoidiO66490.
KOiK00939.
OMAiVLNIEVP.
OrthoDBiEOG679TH4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66490-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILVFLGPPG AGKGTQAKRL AKEKGFVHIS TGDILREAVQ KGTPLGKKAK
60 70 80 90 100
EYMERGELVP DDLIIALIEE VFPKHGNVIF DGFPRTVKQA EALDEMLEKK
110 120 130 140 150
GLKVDHVLLF EVPDEVVIER LSGRRINPET GEVYHVKYNP PPPGVKVIQR
160 170 180 190 200
EDDKPEVIKK RLEVYREQTA PLIEYYKKKG ILRIIDASKP VEEVYRQVLE

VIGDGN
Length:206
Mass (Da):23,231
Last modified:August 1, 1998 - v1
Checksum:i2310B49B11CBC71E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06438.1.
PIRiG70307.
RefSeqiNP_213050.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06438; AAC06438; aq_078.
GeneIDi1192503.
KEGGiaae:aq_078.
PATRICi20957855. VBIAquAeo85532_0068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06438.1 .
PIRi G70307.
RefSeqi NP_213050.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2RGX X-ray 1.90 A 1-206 [» ]
2RH5 X-ray 2.48 A/B/C 1-206 [» ]
3SR0 X-ray 1.56 A/B 1-206 [» ]
4IKE X-ray 1.48 A/B 1-206 [» ]
4JKY X-ray 2.37 A/B 1-203 [» ]
4JL5 X-ray 1.24 A/B 1-203 [» ]
4JL6 X-ray 1.65 A/B 1-203 [» ]
4JL8 X-ray 1.79 A/B 1-203 [» ]
4JLA X-ray 2.12 A/B 1-203 [» ]
4JLB X-ray 1.53 A/B 1-203 [» ]
4JLD X-ray 1.55 A/B 1-203 [» ]
4JLO X-ray 1.73 A/B 1-203 [» ]
4JLP X-ray 1.43 A/B 1-203 [» ]
ProteinModelPortali O66490.
SMRi O66490. Positions 1-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_078.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06438 ; AAC06438 ; aq_078 .
GeneIDi 1192503.
KEGGi aae:aq_078.
PATRICi 20957855. VBIAquAeo85532_0068.

Phylogenomic databases

eggNOGi COG0563.
HOGENOMi HOG000238772.
InParanoidi O66490.
KOi K00939.
OMAi VLNIEVP.
OrthoDBi EOG679TH4.

Enzyme and pathway databases

UniPathwayi UPA00588 ; UER00649 .
BioCyci AAEO224324:GJBH-65-MONOMER.

Miscellaneous databases

EvolutionaryTracei O66490.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00235. Adenylate_kinase_Adk.
InterProi IPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR23359. PTHR23359. 1 hit.
PRINTSi PR00094. ADENYLTKNASE.
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF57774. SSF57774. 1 hit.
TIGRFAMsi TIGR01351. adk. 1 hit.
PROSITEi PS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH BI-SUBSTRATE ANALOG AP5A.

Entry informationi

Entry nameiKAD_AQUAE
AccessioniPrimary (citable) accession number: O66490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3