Reviewed, UniProtKB/Swiss-Prot O66489 (AMPM_AQUAE)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	aq_076

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Methionine aminopeptidase		PRO_0000148924
Sites
	Metal binding	102	1	Cobalt 1 By similarity
	Metal binding	113	1	Cobalt 1 By similarity
	Metal binding	113	1	Cobalt 2 By similarity
	Metal binding	176	1	Cobalt 2 By similarity
	Metal binding	211	1	Cobalt 2 By similarity
	Metal binding	242	1	Cobalt 1 By similarity
	Metal binding	242	1	Cobalt 2 By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66489-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D2EE530C81D09C07	FASTA	258	28,185
	10 20 30 40 50 60 MAIELYSQRE IEKIRKASQI VAEVLHIVAE NVKPGVSTWD LEMIARKETE KRGAKPAFLG 70 80 90 100 110 120 YKPPFSDVRY PAALCISIND EVVHGLPKKE KVIKEGDVVS IDFGAIYDGY AGDSAITVIA 130 140 150 160 170 180 GKGSPEAQKL LEATKEALYN AIEKALPGKK VGDITKAIHE TAEKYGFKTI LRYGGHGVGR 190 200 210 220 230 240 KVHQEPFVPN NVKDIGKKNP RLRQGMVIAI EPMLSIGTEE TVEDGDGWTV KTKDGSLAAH 250 FEHTVAITKK GPVILTEL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06448.1.
PIR	F70307.
RefSeq	NP_213049.1.
3D structure databases
HSSP	HSSP built from PDB template 1O0X based on UniProtKB Q9X1I7.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Genome annotation databases
GeneID	1192502.
GenomeReviews	Gene locus aq_076 in contig AE000657_GR.
KEGG	aae:aq_076.
NMPDR	fig|224324.1.peg.64.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66489.
OMA	AAHSEHT.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_076-MON.
BRENDA	3.4.11.18. 189781.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_AQUAE

Accession

Primary (citable) accession number: O66489

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)