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O66489

- MAP1_AQUAE

UniProt

O66489 - MAP1_AQUAE

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Protein

Methionine aminopeptidase

Gene
map, aq_076
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed By similarity.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei84 – 841Substrate By similarity
Metal bindingi102 – 1021Divalent metal cation 1 By similarity
Metal bindingi113 – 1131Divalent metal cation 1 By similarity
Metal bindingi113 – 1131Divalent metal cation 2; catalytic By similarity
Metal bindingi176 – 1761Divalent metal cation 2; catalytic; via tele nitrogen By similarity
Binding sitei183 – 1831Substrate By similarity
Metal bindingi211 – 2111Divalent metal cation 2; catalytic By similarity
Metal bindingi242 – 2421Divalent metal cation 1 By similarity
Metal bindingi242 – 2421Divalent metal cation 2; catalytic By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-64-MONOMER.

Protein family/group databases

MEROPSiM24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase (EC:3.4.11.18)
Short name:
MAP
Short name:
MetAP
Alternative name(s):
Peptidase M
Gene namesi
Name:map
Ordered Locus Names:aq_076
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Methionine aminopeptidaseUniRule annotationPRO_0000148924Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_076.

Structurei

3D structure databases

ProteinModelPortaliO66489.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000030426.
KOiK01265.
OMAiMPGDRPL.
OrthoDBiEOG6MWNDS.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66489-1 [UniParc]FASTAAdd to Basket

« Hide

MAIELYSQRE IEKIRKASQI VAEVLHIVAE NVKPGVSTWD LEMIARKETE    50
KRGAKPAFLG YKPPFSDVRY PAALCISIND EVVHGLPKKE KVIKEGDVVS 100
IDFGAIYDGY AGDSAITVIA GKGSPEAQKL LEATKEALYN AIEKALPGKK 150
VGDITKAIHE TAEKYGFKTI LRYGGHGVGR KVHQEPFVPN NVKDIGKKNP 200
RLRQGMVIAI EPMLSIGTEE TVEDGDGWTV KTKDGSLAAH FEHTVAITKK 250
GPVILTEL 258
Length:258
Mass (Da):28,185
Last modified:August 1, 1998 - v1
Checksum:iD2EE530C81D09C07
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06448.1.
PIRiF70307.
RefSeqiNP_213049.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06448; AAC06448; aq_076.
GeneIDi1192502.
KEGGiaae:aq_076.
PATRICi20957853. VBIAquAeo85532_0067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06448.1 .
PIRi F70307.
RefSeqi NP_213049.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66489.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_076.

Protein family/group databases

MEROPSi M24.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06448 ; AAC06448 ; aq_076 .
GeneIDi 1192502.
KEGGi aae:aq_076.
PATRICi 20957853. VBIAquAeo85532_0067.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000030426.
KOi K01265.
OMAi MPGDRPL.
OrthoDBi EOG6MWNDS.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-64-MONOMER.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
PROSITEi PS00680. MAP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiMAP1_AQUAE
AccessioniPrimary (citable) accession number: O66489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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