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O66479 (TRMB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:aq_065
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length297 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Domain

The C-terminal region is probably involved in protein stability. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 297297tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171286

Regions

Region165 – 1684Substrate binding By similarity

Sites

Active site971 By similarity
Binding site221S-adenosyl-L-methionine By similarity
Binding site471S-adenosyl-L-methionine By similarity
Binding site741S-adenosyl-L-methionine By similarity
Binding site971S-adenosyl-L-methionine By similarity
Binding site1011Substrate By similarity
Binding site1331Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O66479 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 8CF24B95B3FD1599

FASTA29735,639
        10         20         30         40         50         60 
MLCYVNYKRV KRPVEIPNLE VEIGFGRGDF IVKLAKENPD KNFFGIEISQ ISIEKLMKRV 

        70         80         90        100        110        120 
GKKGLKNVYC TNVDAYWGFY FLFRDNYVEN IYMNYPDPWF KKRHHKRRLT KPERLYMFAK 

       130        140        150        160        170        180 
KLKLGGEIRI RTDNYEFLEF TKESAKVLDC FEVEEGTLNV KEPLTKYEQK WLSMGKTLYK 

       190        200        210        220        230        240 
LILRKVKEPK FVEHPEVEEV RELFPVKVKV ESVDPKKIES REIKLDEEVY FKTFKVWQRD 

       250        260        270        280        290 
KDFLVECLLS EKGYLQKFFI QIKRKEDGYV IDVSPYSEVL RTRNLQRSIQ TVAQLLS 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
[2]"The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA."
Tomikawa C., Hori H.
Nucleic Acids Symp. Ser. 50:245-246(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 204-214, ROLE OF C-TERMINAL REGION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC06452.1.
PIRD70306.
RefSeqNP_213039.1. NC_000918.1.

3D structure databases

ProteinModelPortalO66479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC06452; AAC06452; aq_065.
GeneID1192492.
KEGGaae:aq_065.
PATRIC20957831. VBIAquAeo85532_0056.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000075620.
KOK03439.
OMALEVEIGF.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-54-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_AQUAE
AccessionPrimary (citable) accession number: O66479
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways