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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22S-adenosyl-L-methionineUniRule annotation1
Binding sitei47S-adenosyl-L-methionineUniRule annotation1
Binding sitei74S-adenosyl-L-methionineUniRule annotation1
Active sitei97By similarity1
Binding sitei97S-adenosyl-L-methionineUniRule annotation1
Binding sitei101SubstrateUniRule annotation1
Binding sitei133SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.33. 396.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:aq_065
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712861 – 297tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST297

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_065.

Structurei

3D structure databases

ProteinModelPortaliO66479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 168Substrate bindingUniRule annotation4

Domaini

The C-terminal region is probably involved in protein stability.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000075620.
InParanoidiO66479.
KOiK03439.
OMAiIVHMAKE.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCYVNYKRV KRPVEIPNLE VEIGFGRGDF IVKLAKENPD KNFFGIEISQ
60 70 80 90 100
ISIEKLMKRV GKKGLKNVYC TNVDAYWGFY FLFRDNYVEN IYMNYPDPWF
110 120 130 140 150
KKRHHKRRLT KPERLYMFAK KLKLGGEIRI RTDNYEFLEF TKESAKVLDC
160 170 180 190 200
FEVEEGTLNV KEPLTKYEQK WLSMGKTLYK LILRKVKEPK FVEHPEVEEV
210 220 230 240 250
RELFPVKVKV ESVDPKKIES REIKLDEEVY FKTFKVWQRD KDFLVECLLS
260 270 280 290
EKGYLQKFFI QIKRKEDGYV IDVSPYSEVL RTRNLQRSIQ TVAQLLS
Length:297
Mass (Da):35,639
Last modified:August 1, 1998 - v1
Checksum:i8CF24B95B3FD1599
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06452.1.
PIRiD70306.
RefSeqiNP_213039.1. NC_000918.1.
WP_010879977.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065.
GeneIDi1192492.
KEGGiaae:aq_065.
PATRICi20957831. VBIAquAeo85532_0056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06452.1.
PIRiD70306.
RefSeqiNP_213039.1. NC_000918.1.
WP_010879977.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065.
GeneIDi1192492.
KEGGiaae:aq_065.
PATRICi20957831. VBIAquAeo85532_0056.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000075620.
InParanoidiO66479.
KOiK03439.
OMAiIVHMAKE.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.
BRENDAi2.1.1.33. 396.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_AQUAE
AccessioniPrimary (citable) accession number: O66479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.