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O66479

- TRMB_AQUAE

UniProt

O66479 - TRMB_AQUAE

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221S-adenosyl-L-methionineUniRule annotation
Binding sitei47 – 471S-adenosyl-L-methionineUniRule annotation
Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
Active sitei97 – 971By similarity
Binding sitei97 – 971S-adenosyl-L-methionineUniRule annotation
Binding sitei101 – 1011SubstrateUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-54-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:aq_065
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297tRNA (guanine-N(7)-)-methyltransferasePRO_0000171286Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_065.

Structurei

3D structure databases

ProteinModelPortaliO66479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 1684Substrate bindingUniRule annotation

Domaini

The C-terminal region is probably involved in protein stability.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000075620.
InParanoidiO66479.
KOiK03439.
OMAiLEVEIGF.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66479-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLCYVNYKRV KRPVEIPNLE VEIGFGRGDF IVKLAKENPD KNFFGIEISQ
60 70 80 90 100
ISIEKLMKRV GKKGLKNVYC TNVDAYWGFY FLFRDNYVEN IYMNYPDPWF
110 120 130 140 150
KKRHHKRRLT KPERLYMFAK KLKLGGEIRI RTDNYEFLEF TKESAKVLDC
160 170 180 190 200
FEVEEGTLNV KEPLTKYEQK WLSMGKTLYK LILRKVKEPK FVEHPEVEEV
210 220 230 240 250
RELFPVKVKV ESVDPKKIES REIKLDEEVY FKTFKVWQRD KDFLVECLLS
260 270 280 290
EKGYLQKFFI QIKRKEDGYV IDVSPYSEVL RTRNLQRSIQ TVAQLLS
Length:297
Mass (Da):35,639
Last modified:August 1, 1998 - v1
Checksum:i8CF24B95B3FD1599
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06452.1.
PIRiD70306.
RefSeqiNP_213039.1. NC_000918.1.
WP_010879977.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065.
GeneIDi1192492.
KEGGiaae:aq_065.
PATRICi20957831. VBIAquAeo85532_0056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06452.1 .
PIRi D70306.
RefSeqi NP_213039.1. NC_000918.1.
WP_010879977.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66479.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_065.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06452 ; AAC06452 ; aq_065 .
GeneIDi 1192492.
KEGGi aae:aq_065.
PATRICi 20957831. VBIAquAeo85532_0056.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000075620.
InParanoidi O66479.
KOi K03439.
OMAi LEVEIGF.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci AAEO224324:GJBH-54-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA."
    Tomikawa C., Hori H.
    Nucleic Acids Symp. Ser. 50:245-246(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 204-214, ROLE OF C-TERMINAL REGION.

Entry informationi

Entry nameiTRMB_AQUAE
AccessioniPrimary (citable) accession number: O66479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3