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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221S-adenosyl-L-methionineUniRule annotation
Binding sitei47 – 471S-adenosyl-L-methionineUniRule annotation
Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
Active sitei97 – 971By similarity
Binding sitei97 – 971S-adenosyl-L-methionineUniRule annotation
Binding sitei101 – 1011SubstrateUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-54-MONOMER.
BRENDAi2.1.1.33. 396.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:aq_065
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297tRNA (guanine-N(7)-)-methyltransferasePRO_0000171286Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_065.

Structurei

3D structure databases

ProteinModelPortaliO66479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 1684Substrate bindingUniRule annotation

Domaini

The C-terminal region is probably involved in protein stability.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000075620.
InParanoidiO66479.
KOiK03439.
OMAiIFLEIGM.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCYVNYKRV KRPVEIPNLE VEIGFGRGDF IVKLAKENPD KNFFGIEISQ
60 70 80 90 100
ISIEKLMKRV GKKGLKNVYC TNVDAYWGFY FLFRDNYVEN IYMNYPDPWF
110 120 130 140 150
KKRHHKRRLT KPERLYMFAK KLKLGGEIRI RTDNYEFLEF TKESAKVLDC
160 170 180 190 200
FEVEEGTLNV KEPLTKYEQK WLSMGKTLYK LILRKVKEPK FVEHPEVEEV
210 220 230 240 250
RELFPVKVKV ESVDPKKIES REIKLDEEVY FKTFKVWQRD KDFLVECLLS
260 270 280 290
EKGYLQKFFI QIKRKEDGYV IDVSPYSEVL RTRNLQRSIQ TVAQLLS
Length:297
Mass (Da):35,639
Last modified:August 1, 1998 - v1
Checksum:i8CF24B95B3FD1599
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06452.1.
PIRiD70306.
RefSeqiNP_213039.1. NC_000918.1.
WP_010879977.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065.
GeneIDi1192492.
KEGGiaae:aq_065.
PATRICi20957831. VBIAquAeo85532_0056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06452.1.
PIRiD70306.
RefSeqiNP_213039.1. NC_000918.1.
WP_010879977.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065.
GeneIDi1192492.
KEGGiaae:aq_065.
PATRICi20957831. VBIAquAeo85532_0056.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000075620.
InParanoidiO66479.
KOiK03439.
OMAiIFLEIGM.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciAAEO224324:GJBH-54-MONOMER.
BRENDAi2.1.1.33. 396.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA."
    Tomikawa C., Hori H.
    Nucleic Acids Symp. Ser. 50:245-246(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 204-214, ROLE OF C-TERMINAL REGION.

Entry informationi

Entry nameiTRMB_AQUAE
AccessioniPrimary (citable) accession number: O66479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: May 27, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.