Reviewed, UniProtKB/Swiss-Prot O66461 (PYRD_AQUAE)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	aq_046

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00224
Subunit structure	Heterotetramer of 2 pyrK and 2 pyrD subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Dihydroorotate dehydrogenase HAMAP MF_00224		PRO_0000148386
Sites
	Active site	129	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66461-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 3E1E04D8DE1FCD53	FASTA	306	33,523
	10 20 30 40 50 60 MDLSVELFGI RFKNPVWVAS GTFGYGVEAA EIYDISKLGA VVTKGLSLKE RLGNETPRIV 70 80 90 100 110 120 ETPCGMLNSI GLQNPGVEKF LKEIYPKIKD VDTHFIANVF GETEEEYVEV CMALEDADKI 130 140 150 160 170 180 VAYELNVSCP NVKKGGILFG HDPVILGNLV DRIKAKIKKP LLVKLSPNVT DVTEFAKVCI 190 200 210 220 230 240 ENGADGLVLI NTLMGMKINI WKRKPDLATK TGGLSGPAIL PIAVRMIYQV YEKFGDRIPI 250 260 270 280 290 300 IGVGGITTWE DAMEHVMAGA SAVQVGTANF YEPLAPLKVI EGIENFMKSQ NIKDFKELIG IAHRVE

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06426.1.
PIR	B70304.
RefSeq	NP_213021.1.
3D structure databases
HSSP	HSSP built from PDB template 1EP3 based on UniProtKB P54322.
ModBase	Search...
Genome annotation databases
GeneID	1192658.
GenomeReviews	Gene locus aq_046 in contig AE000657_GR.
KEGG	aae:aq_046.
NMPDR	fig|224324.1.peg.36.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66461.
OMA	O66461. NSIGLQN.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_046-MON.
BRENDA	1.3.3.1. 189781.
Family and domain databases
HAMAP	MF_00224. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AQUAE

Accession

Primary (citable) accession number: O66461

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)