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O66164 (FTHS_SPHPI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Synonyms:ligH
OrganismSphingomonas paucimobilis (Pseudomonas paucimobilis)
Taxonomic identifier13689 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 557557Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199370

Regions

Nucleotide binding67 – 748ATP By similarity

Experimental info

Sequence conflict13 – 142ER → AK in BAD61061. Ref.2
Sequence conflict1751V → AL in BAD61061. Ref.2
Sequence conflict4271H → L in BAD61061. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O66164 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: D296E7538F6C8E86

FASTA55759,423
        10         20         30         40         50         60 
MAQGSDIEIA REERMQNIAE VGAKVGIPQD ALLNYGPYKA KLSWDFINSV QGNQDGKLIL 

        70         80         90        100        110        120 
VTAINPTPAG EGKTTTTVGL ADGLNRIGKK TVAALREPSL GPCFGVKGGA AGGGYAQVVP 

       130        140        150        160        170        180 
MEDINLHFTG DFHAITSANN LLAALIDNHI YWGNKLGLDP RRIAWRRVLD MNDRVRSIVN 

       190        200        210        220        230        240 
SLGGVSNGYP REDGFDITVA SEVMAILCLS SDLKDLERRL GNIHAGYTRE RKAVLASELN 

       250        260        270        280        290        300 
ASGAMTVLLK DALQPNMVQT LENNPVLIHG GPFANIAHGC NSVLATKTAL KIADYVVTEA 

       310        320        330        340        350        360 
GFGADLGAEK FFDIKCRKAG LKPSAAVIVA TIRALKMHGG VDKADLGTAN PEAVRKGGVN 

       370        380        390        400        410        420 
LARHIENVRQ FGVPVVVAIN QFITDTDEEM AMVKEIAEAA GAEAVLCSHW ANGSAGTEEL 

       430        440        450        460        470        480 
ARKVVAHAES GSSNFAPLYE DSMPLFEKID TIAKRIYRAT EATADSSVRN KLKGWEADGF 

       490        500        510        520        530        540 
GHLPVCMAKT QYSFSTDPAL RGAPTDHVVP VRDVILSAGA EFIVAVCGDI MRMPGLPKVP 

       550 
SADFIKLDEQ GQIQGLF 

« Hide

References

[1]"Cloning and sequencing of the Sphingomonas (Pseudomonas) paucimobilis gene essential for the O demethylation of vanillate and syringate."
Nishikawa S., Sonoki T., Kasahara T., Obi T., Kubota S., Kawai S., Morohoshi N., Katayama Y.
Appl. Environ. Microbiol. 64:836-842(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SYK-6.
[2]"A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6."
Abe T., Masai E., Miyauchi K., Katayama Y., Fukuda M.
J. Bacteriol. 187:2030-2037(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SYK-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006079 Genomic DNA. Translation: BAA25140.1.
AB186750 Genomic DNA. Translation: BAD61061.1.

3D structure databases

ProteinModelPortalO66164.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_SPHPI
AccessionPrimary (citable) accession number: O66164
Secondary accession number(s): Q60FW9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways