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O66164

- FTHS_SPHPI

UniProt

O66164 - FTHS_SPHPI

Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Sphingomonas paucimobilis (Pseudomonas paucimobilis)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi67 – 748ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
    Alternative name(s):
    Formyltetrahydrofolate synthetaseUniRule annotation
    Short name:
    FHSUniRule annotation
    Short name:
    FTHFSUniRule annotation
    Gene namesi
    Name:fhsUniRule annotation
    Synonyms:ligH
    OrganismiSphingomonas paucimobilis (Pseudomonas paucimobilis)
    Taxonomic identifieri13689 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 557557Formate--tetrahydrofolate ligasePRO_0000199370Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO66164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66164-1 [UniParc]FASTAAdd to Basket

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    MAQGSDIEIA REERMQNIAE VGAKVGIPQD ALLNYGPYKA KLSWDFINSV    50
    QGNQDGKLIL VTAINPTPAG EGKTTTTVGL ADGLNRIGKK TVAALREPSL 100
    GPCFGVKGGA AGGGYAQVVP MEDINLHFTG DFHAITSANN LLAALIDNHI 150
    YWGNKLGLDP RRIAWRRVLD MNDRVRSIVN SLGGVSNGYP REDGFDITVA 200
    SEVMAILCLS SDLKDLERRL GNIHAGYTRE RKAVLASELN ASGAMTVLLK 250
    DALQPNMVQT LENNPVLIHG GPFANIAHGC NSVLATKTAL KIADYVVTEA 300
    GFGADLGAEK FFDIKCRKAG LKPSAAVIVA TIRALKMHGG VDKADLGTAN 350
    PEAVRKGGVN LARHIENVRQ FGVPVVVAIN QFITDTDEEM AMVKEIAEAA 400
    GAEAVLCSHW ANGSAGTEEL ARKVVAHAES GSSNFAPLYE DSMPLFEKID 450
    TIAKRIYRAT EATADSSVRN KLKGWEADGF GHLPVCMAKT QYSFSTDPAL 500
    RGAPTDHVVP VRDVILSAGA EFIVAVCGDI MRMPGLPKVP SADFIKLDEQ 550
    GQIQGLF 557
    Length:557
    Mass (Da):59,423
    Last modified:August 1, 1998 - v1
    Checksum:iD296E7538F6C8E86
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 142ER → AK in BAD61061. (PubMed:15743951)Curated
    Sequence conflicti175 – 1751V → AL in BAD61061. (PubMed:15743951)Curated
    Sequence conflicti427 – 4271H → L in BAD61061. (PubMed:15743951)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006079 Genomic DNA. Translation: BAA25140.1.
    AB186750 Genomic DNA. Translation: BAD61061.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006079 Genomic DNA. Translation: BAA25140.1 .
    AB186750 Genomic DNA. Translation: BAD61061.1 .

    3D structure databases

    ProteinModelPortali O66164.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_01543. FTHFS.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the Sphingomonas (Pseudomonas) paucimobilis gene essential for the O demethylation of vanillate and syringate."
      Nishikawa S., Sonoki T., Kasahara T., Obi T., Kubota S., Kawai S., Morohoshi N., Katayama Y.
      Appl. Environ. Microbiol. 64:836-842(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SYK-6.
    2. "A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6."
      Abe T., Masai E., Miyauchi K., Katayama Y., Fukuda M.
      J. Bacteriol. 187:2030-2037(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SYK-6.

    Entry informationi

    Entry nameiFTHS_SPHPI
    AccessioniPrimary (citable) accession number: O66164
    Secondary accession number(s): Q60FW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3