O66143 (ARGA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	BU456

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subunit structure	Homohexamer Probable.
Subcellular location	Cytoplasm Probable HAMAP MF_01105.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186788
Regions
	Domain	295 – 442	148	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	O66143 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: C7F67E99C285A394	FASTA	442	49,226
	10 20 30 40 50 60 MKERNTELVQ GFRHSVPYIN AHRGKTFVIM LGGEAIKYGN FYSIINDIGL LHSLGIRLVV 70 80 90 100 110 120 VYGACPQINT SLKEKNIKII YHKSIRITDL ASLEQVKQAA GKLQLDITAR LSMSLTNTPL 130 140 150 160 170 180 QGANISVVSG NFIISQPLGV DDGVDYCHSG RVRRIDKNAI NCQLNNGAIV LIGPVAVSVT 190 200 210 220 230 240 GESFNLTSEE IATQVSIELK AEKMIGFCGN QGVINDEGKI ISELLSNDIK NIIKKLEKKG 250 260 270 280 290 300 DYISSTVRFL KGSIKACKSG VNRSHLISYH KSGALLQELF SRDGIGTQMV MESAEKIRGA 310 320 330 340 350 360 SINDIGGILE LIRPLEHKGI LVRRSREQLE IEVDKFTIIE HDNLTIACAA LYPFFKEKIG 370 380 390 400 410 420 EMACLAVHPD YRNSSRGDAL LKKIKMNAKD MHLKRIFVLT TQSIHWFQER GFILVDIEVL 430 440 PESKKKMYNY QRGSKILMID VI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
[2]	"Differential display of mRNAs related to amino acid metabolism in the endosymbiotic system of aphids." Nakabachi A., Ishikawa H. Insect Biochem. Mol. Biol. 27:1057-1062(1997) [PubMed: 9569646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-71.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13154.1. AB005263 mRNA. Translation: BAA25386.1.
RefSeq	NP_240268.1. NC_002528.1.
3D structure databases
ProteinModelPortal	O66143.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002817; EBBUCP00000002593; EBBUCG00000002817.
GeneID	1109804.
GenomeReviews	Gene locus BU456 in contig BA000003_GR.
KEGG	buc:BU456.
PATRIC	21244454. VBIBucAph127364_0466.
Phylogenomic databases
GeneTree	EBGT00050000007812.
HOGENOM	HBG545264.
OMA	AFNLAME.
PhylomeDB	O66143.
ProtClustDB	PRK05279.
Enzyme and pathway databases
BioCyc	BSP107806:BU456-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_BUCAI

Accession

Primary (citable) accession number: O66143

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program