O66132 (KHSE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BU193

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Homoserine kinase HAMAP MF_00384		PRO_0000156555
Regions
	Nucleotide binding	91 – 101	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O66132 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 1AAF441544F16712	FASTA	309	34,091
	10 20 30 40 50 60 MIKIYAPASI GNVGVGFDIL GAAIIPVNGS LLGDFVTVKL SNKFNLVNKG IFSNKLPKNT 70 80 90 100 110 120 EQNIVWKCWL KFCNTIKRNI PVSIILEKNM PIGSGLGSSA CSIVATLVAM NEFCDKPLNS 130 140 150 160 170 180 KELLLLMGEV EGEISGSIHY DNVAPCYLGG LQLILEDSKI ISQTIPNFKN WFWIVAWPGT 190 200 210 220 230 240 KVPTAEARDI LPKKYKKETC IKNSRYLAGF IHASYSQQPH LAARLMQDFI AEPYRIKLLP 250 260 270 280 290 300 NYLYVKEKIK KIGAISSGIS GSGPTIFSIS DNINTAQKIS AWLTENYLQN TTGFVHICFL DSKGVRKIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
[2]	"Differential display of mRNAs related to amino acid metabolism in the endosymbiotic system of aphids." Nakabachi A., Ishikawa H. Insect Biochem. Mol. Biol. 27:1057-1062(1997) [PubMed: 9569646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-244.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12910.1. AB004856 mRNA. Translation: BAA25384.1.
RefSeq	NP_240024.1. NC_002528.1.
3D structure databases
ProteinModelPortal	O66132.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003294; EBBUCP00000003070; EBBUCG00000003294.
GeneID	1109636.
GenomeReviews	Gene locus BU193 in contig BA000003_GR.
KEGG	buc:BU193.
PATRIC	21243904. VBIBucAph127364_0204.
Phylogenomic databases
GeneTree	EBGT00050000007703.
HOGENOM	HBG646290.
OMA	GSAHADN.
PhylomeDB	O66132.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	BSP107806:BU193-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BUCAI

Accession

Primary (citable) accession number: O66132

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program