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O66113

- ODPB_ZYMMO

UniProt

O66113 - ODPB_ZYMMO

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Protein

Pyruvate dehydrogenase E1 component subunit beta

Gene

pdhB

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951Thiamine pyrophosphateBy similarity

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

GO - Biological processi

  1. acetyl-CoA biosynthetic process from pyruvate Source: InterPro
  2. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1993-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta (EC:1.2.4.1)
Gene namesi
Name:pdhB
Synonyms:pdhAbeta
Ordered Locus Names:ZMO1605
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Pyruvate dehydrogenase E1 component subunit betaPRO_0000162237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431N6-lipoyllysinePROSITE-ProRule annotation

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

STRINGi264203.ZMO1605.

Structurei

3D structure databases

ProteinModelPortaliO66113.
SMRiO66113. Positions 138-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7877Lipoyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 lipoyl-binding domain.CuratedPROSITE-ProRule annotation

Keywords - Domaini

Lipoyl

Phylogenomic databases

eggNOGiCOG0508.
HOGENOMiHOG000281450.
KOiK00162.
OMAiYASWYAH.
OrthoDBiEOG6JQH4C.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR027110. PDHB.
IPR011053. Single_hybrid_motif.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view]
PANTHERiPTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamiPF00364. Biotin_lipoyl. 1 hit.
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66113-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIELKMPAL SPTMEEGTLT RWLVKEGDSI KAGEILAEIE TDKAIMEFEA
60 70 80 90 100
VDEGVITKIL IPEGSENVKV GTAIAYLGTD ANDVTLDGAS AETKAEESAP
110 120 130 140 150
VASPAKTEAA AVEEAATPSL GKVINSAPEI PEGTEFFQQT LREALRDAMA
160 170 180 190 200
EEMRRDDRVF VMGEEVAEYQ GAYKVTQGLL QEFGARRVVD TPISEYGFSG
210 220 230 240 250
IGVGAAMEGL RPVIEFMTMN FSMQAIDHII NSAAKTHYMS GGQVRCPIVF
260 270 280 290 300
RGPNGAAPRV GAQHTQNFGP WYAAVPGLVV LAPYDAIDAK GLLKAAIRSD
310 320 330 340 350
DPVVFLECEL LYGKTFDVPK MDDFVLPIGK ARIIREGKDV TIVSYSIGVS
360 370 380 390 400
FALTAAEALA KEGIDAEVID LRTLRPLDKE TILQSLAKTN RIVTVEDGWP
410 420 430 440 450
VCSISSEIAA IAMEEGFDNL DAPVLRVTNA DTPTPYAENL EKKGLVNPEA
460
IIEAVRKVCY RK
Length:462
Mass (Da):49,833
Last modified:February 15, 2005 - v2
Checksum:i00F53CE4A5731F1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871D → E in CAA73385. (PubMed:9515924)Curated
Sequence conflicti112 – 1121V → L in CAA73385. (PubMed:9515924)Curated
Sequence conflicti232 – 24413SAAKT…SGGQV → LRPKRIICPAAKC1 PublicationCuratedAdd
BLAST
Sequence conflicti259 – 2591R → P in AAC70362. 1 PublicationCurated
Sequence conflicti262 – 2621A → E in AAC70362. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12884 Genomic DNA. Translation: CAA73385.1.
AF086791 Genomic DNA. Translation: AAC70362.1.
AE008692 Genomic DNA. Translation: AAV90229.1.
PIRiT33723.
RefSeqiYP_163340.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV90229; AAV90229; ZMO1605.
GeneIDi3187757.
KEGGizmo:ZMO1605.
PATRICi32568468. VBIZymMob102260_1510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12884 Genomic DNA. Translation: CAA73385.1 .
AF086791 Genomic DNA. Translation: AAC70362.1 .
AE008692 Genomic DNA. Translation: AAV90229.1 .
PIRi T33723.
RefSeqi YP_163340.1. NC_006526.2.

3D structure databases

ProteinModelPortali O66113.
SMRi O66113. Positions 138-459.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264203.ZMO1605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV90229 ; AAV90229 ; ZMO1605 .
GeneIDi 3187757.
KEGGi zmo:ZMO1605.
PATRICi 32568468. VBIZymMob102260_1510.

Phylogenomic databases

eggNOGi COG0508.
HOGENOMi HOG000281450.
KOi K00162.
OMAi YASWYAH.
OrthoDBi EOG6JQH4C.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-1993-MONOMER.

Family and domain databases

Gene3Di 3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProi IPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR027110. PDHB.
IPR011053. Single_hybrid_motif.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view ]
PANTHERi PTHR11624:SF56. PTHR11624:SF56. 1 hit.
Pfami PF00364. Biotin_lipoyl. 1 hit.
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view ]
SMARTi SM00861. Transket_pyr. 1 hit.
[Graphical view ]
SUPFAMi SSF51230. SSF51230. 1 hit.
SSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
PROSITEi PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification of the pyruvate dehydrogenase multienzyme complex of Zymomonas mobilis and identification and sequence analysis of the corresponding genes."
    Neveling U., Klasen R., Bringer-Meyer S., Sahm H.
    J. Bacteriol. 180:1540-1548(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29191 / DSM 3580 / NBRC 13756 / NCIMB 11199 / NRRL B-4490 / ZM6.
  2. Lee J., Jin S., Kang H.S.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiODPB_ZYMMO
AccessioniPrimary (citable) accession number: O66113
Secondary accession number(s): O69012, Q5NM31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 15, 2005
Last modified: November 26, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3