Reviewed, UniProtKB/Swiss-Prot O66112 (ODPA_ZYMMO)

Names and origin

Protein names

Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha
    EC=1.2.4.1

Gene names

Name:	pdhA
Synonyms:	pdhAalpha
Ordered Locus Names:	ZMO1606

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Heterodimer of an alpha and a beta chain.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular membrane-bounded organelle Inferred from electronic annotation. Source: InterPro
Molecular function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Pyruvate dehydrogenase E1 component subunit alpha		PRO_0000162213
Experimental info
	Sequence conflict	69	1	C → N in AAC70361. Ref.2
	Sequence conflict	309	1	Missing in AAC70361. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O66112-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 515F5B2EDA0C1424	FASTA	354	38,654
	10 20 30 40 50 60 MAKATQDSNR PHKADVGSAI PNHDLPPIPG RYHADREELL EFYRRMLMIR RFEERCGQLY 70 80 90 100 110 120 GLGLIAGFCH LYIGQEAVAV GLQAALQPGR DSVITGYREH GHMLAYGIDP KIVMAELTGR 130 140 150 160 170 180 ASGISHGKGG SMHMFSTEHK FFGGNGIVGA QVPLGAGLAF AHKYRNDGGC SAAYFGDGSA 190 200 210 220 230 240 NQGQVYEAYN MAALWKLPVI FVIENNGYAM GTSIQRANAH TALSERGAGF GIPALVVDGM 250 260 270 280 290 300 DVLEVRGAAT VAVDWVQAGK GPIILEMKTY RYRGHSMSDP ARYRSREEVN DMKENHDPLD 310 320 330 340 350 NLKKDLFAAG VPEAELVKLD EDIRQQVKEA ADFAEKAPLP ADEELYTNIL VGKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification of the pyruvate dehydrogenase multienzyme complex of Zymomonas mobilis and identification and sequence analysis of the corresponding genes." Neveling U., Klasen R., Bringer-Meyer S., Sahm H. J. Bacteriol. 180:1540-1548(1998) [PubMed: 9515924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29191 / DSM 3580 / IFO 13756 / NCIB 11199 / ZM6.
[2]	Lee J., Jin S., Kang H.S. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[3]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y12884 Genomic DNA. Translation: CAA73384.1. AF086791 Genomic DNA. Translation: AAC70361.1. AE008692 Genomic DNA. Translation: AAV90230.1.
PIR	T33722.
RefSeq	YP_163341.1.
3D structure databases
HSSP	HSSP built from PDB template 1NI4 based on UniProtKB P08559.
ModBase	Search...
Genome annotation databases
GeneID	3187783.
GenomeReviews	Gene locus ZMO1606 in contig AE008692_GR.
KEGG	zmo:ZMO1606.
NMPDR	fig|264203.3.peg.80.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66112.
OMA	LEYETYR.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO1606-MON.
BRENDA	1.2.4.1. 1658.
Family and domain databases
InterPro	IPR001017. DH_E1. IPR017597. Pyrv_DH_E1_asu_subgrp-y. [Graphical view]
Pfam	PF00676. E1_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03182. PDH_E1_alph_y. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODPA_ZYMMO

Accession

Primary (citable) accession number: O66112
Secondary accession number(s): O69011, Q5NM30

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)