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O66108 (TYSY_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:VC_0675
OrganismVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]
Taxonomic identifier243277 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000141039

Sites

Active site1601 By similarity

Natural variations

Natural variant1871G → W in inactive enzyme. Ref.3

Experimental info

Sequence conflict21R → K in CAA07073. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O66108 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: D50B760285FDE97E

FASTA28332,573
        10         20         30         40         50         60 
MRQYLDLCQR IVDQGVWVEN ERTGKRCLTV INADLTYDVG NNQFPLVTTR KSFWKAAVAE 

        70         80         90        100        110        120 
LLGYIRGYDN AADFRQLGTK TWDANANLNQ AWLNNPYRKG EDDMGRVYGV QGRAWAKPDG 

       130        140        150        160        170        180 
GHIDQLKKIV DDLSRGVDDR GEILNFYNPG EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR 

       190        200        210        220        230        240 
SCDVPLGLNF NMVQVYVFLA LMAQITGKKP GLAYHKIVNA HIYQDQLELM RDVQLKREPF 

       250        260        270        280 
PAPQFHINPK IKTLQDLETW VTLDDFDVTG YQFHDPIQYP FSV 

« Hide

References

« Hide 'large scale' references
[1]Valle E., Silva A., Benitez J.A.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: El Tor C7258 / Serotype O1.
[2]"Characterisation of the lgt/thyA locus from Vibrio cholerae."
Carlin N.I.A., Nilsson A., Todorovic M., Holmgren J., Lebens M.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: JS1569.
[3]Xia X.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT TRP-187.
[4]"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae."
Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. expand/collapse author list , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.
Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y17135 Genomic DNA. Translation: CAA76645.1.
AJ006514 Genomic DNA. Translation: CAA07073.1.
AY143429 Genomic DNA. Translation: AAN16904.1.
AE003852 Genomic DNA. Translation: AAF93840.1.
PIRC82295.
RefSeqNP_230324.1. NC_002505.1.

3D structure databases

ProteinModelPortalO66108.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243277.VC0675.

Protocols and materials databases

DNASU2615464.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF93840; AAF93840; VC_0675.
GeneID2615464.
KEGGvch:VC0675.
PATRIC20080467. VBIVibCho83274_0647.

Phylogenomic databases

eggNOGCOG0207.
KOK00560.
OMAENYVADA.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycVCHO:VC0675-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_VIBCH
AccessionPrimary (citable) accession number: O66108
Secondary accession number(s): O87078, Q8GKX5, Q9JQ12
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways