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O66108

- TYSY_VIBCH

UniProt

O66108 - TYSY_VIBCH

Protein

Thymidylate synthase

Gene

thyA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei160 – 1601UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: TIGR

    GO - Biological processi

    1. 2'-deoxyribonucleotide biosynthetic process Source: TIGR
    2. dTMP biosynthetic process Source: UniProtKB-HAMAP
    3. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciVCHO:VC0675-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:VC_0675
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    Taxonomic identifieri243277 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000584: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 283283Thymidylate synthasePRO_0000141039Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243277.VC0675.

    Structurei

    3D structure databases

    ProteinModelPortaliO66108.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    KOiK00560.
    OMAiENYVADA.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66108-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQYLDLCQR IVDQGVWVEN ERTGKRCLTV INADLTYDVG NNQFPLVTTR    50
    KSFWKAAVAE LLGYIRGYDN AADFRQLGTK TWDANANLNQ AWLNNPYRKG 100
    EDDMGRVYGV QGRAWAKPDG GHIDQLKKIV DDLSRGVDDR GEILNFYNPG 150
    EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVFLA 200
    LMAQITGKKP GLAYHKIVNA HIYQDQLELM RDVQLKREPF PAPQFHINPK 250
    IKTLQDLETW VTLDDFDVTG YQFHDPIQYP FSV 283
    Length:283
    Mass (Da):32,573
    Last modified:August 1, 1998 - v1
    Checksum:iD50B760285FDE97E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21R → K in CAA07073. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti187 – 1871G → W in inactive enzyme. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17135 Genomic DNA. Translation: CAA76645.1.
    AJ006514 Genomic DNA. Translation: CAA07073.1.
    AY143429 Genomic DNA. Translation: AAN16904.1.
    AE003852 Genomic DNA. Translation: AAF93840.1.
    PIRiC82295.
    RefSeqiNP_230324.1. NC_002505.1.
    WP_001250353.1. NC_002505.1.

    Genome annotation databases

    EnsemblBacteriaiAAF93840; AAF93840; VC_0675.
    GeneIDi2615464.
    KEGGivch:VC0675.
    PATRICi20080467. VBIVibCho83274_0647.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17135 Genomic DNA. Translation: CAA76645.1 .
    AJ006514 Genomic DNA. Translation: CAA07073.1 .
    AY143429 Genomic DNA. Translation: AAN16904.1 .
    AE003852 Genomic DNA. Translation: AAF93840.1 .
    PIRi C82295.
    RefSeqi NP_230324.1. NC_002505.1.
    WP_001250353.1. NC_002505.1.

    3D structure databases

    ProteinModelPortali O66108.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243277.VC0675.

    Protocols and materials databases

    DNASUi 2615464.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF93840 ; AAF93840 ; VC_0675 .
    GeneIDi 2615464.
    KEGGi vch:VC0675.
    PATRICi 20080467. VBIVibCho83274_0647.

    Phylogenomic databases

    eggNOGi COG0207.
    KOi K00560.
    OMAi ENYVADA.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci VCHO:VC0675-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Valle E., Silva A., Benitez J.A.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: El Tor C7258 / Serotype O1.
    2. "Characterisation of the lgt/thyA locus from Vibrio cholerae."
      Carlin N.I.A., Nilsson A., Todorovic M., Holmgren J., Lebens M.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: JS1569.
    3. Xia X.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT TRP-187.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39315 / El Tor Inaba N16961.

    Entry informationi

    Entry nameiTYSY_VIBCH
    AccessioniPrimary (citable) accession number: O66108
    Secondary accession number(s): O87078, Q8GKX5, Q9JQ12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3