O66108 (TYSY_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	VC_0675

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	2'-deoxyribonucleotide biosynthetic process Inferred from sequence or structural similarity Ref.4. Source: TIGR dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from sequence or structural similarity Ref.4. Source: TIGR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000141039
Sites
	Active site	160	1	By similarity
Natural variations
	Natural variant	187	1	G → W in inactive enzyme. Ref.3
Experimental info
	Sequence conflict	2	1	R → K in CAA07073. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O66108 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D50B760285FDE97E	FASTA	283	32,573
	10 20 30 40 50 60 MRQYLDLCQR IVDQGVWVEN ERTGKRCLTV INADLTYDVG NNQFPLVTTR KSFWKAAVAE 70 80 90 100 110 120 LLGYIRGYDN AADFRQLGTK TWDANANLNQ AWLNNPYRKG EDDMGRVYGV QGRAWAKPDG 130 140 150 160 170 180 GHIDQLKKIV DDLSRGVDDR GEILNFYNPG EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR 190 200 210 220 230 240 SCDVPLGLNF NMVQVYVFLA LMAQITGKKP GLAYHKIVNA HIYQDQLELM RDVQLKREPF 250 260 270 280 PAPQFHINPK IKTLQDLETW VTLDDFDVTG YQFHDPIQYP FSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Valle E., Silva A., Benitez J.A. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor C7258 / Serotype O1.
[2]	"Characterisation of the lgt/thyA locus from Vibrio cholerae." Carlin N.I.A., Nilsson A., Todorovic M., Holmgren J., Lebens M. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JS1569.
[3]	Xia X. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT TRP-187.
[4]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17135 Genomic DNA. Translation: CAA76645.1. AJ006514 Genomic DNA. Translation: CAA07073.1. AY143429 Genomic DNA. Translation: AAN16904.1. AE003852 Genomic DNA. Translation: AAF93840.1.
PIR	C82295.
RefSeq	NP_230324.1. NC_002505.1.
3D structure databases
ProteinModelPortal	O66108.
ModBase	Search...
Protein-protein interaction databases
STRING	243277.VC0675.
Protocols and materials databases
DNASU	2615464.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF93840; AAF93840; VC_0675.
GeneID	2615464.
KEGG	vch:VC0675.
PATRIC	20080467. VBIVibCho83274_0647.
Phylogenomic databases
eggNOG	COG0207.
KO	K00560.
OMA	KSWDANA.
ProtClustDB	PRK01827.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_VIBCH

Accession

Primary (citable) accession number: O66108
Secondary accession number(s): O87078, Q8GKX5, Q9JQ12

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program