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Protein

Thymidylate synthase

Gene

thyA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: TIGR

GO - Biological processi

  1. 2'-deoxyribonucleotide biosynthetic process Source: TIGR
  2. dTMP biosynthetic process Source: UniProtKB-HAMAP
  3. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciVCHO:VC0675-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:VC_0675
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Thymidylate synthasePRO_0000141039Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243277.VC0675.

Structurei

3D structure databases

ProteinModelPortaliO66108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQYLDLCQR IVDQGVWVEN ERTGKRCLTV INADLTYDVG NNQFPLVTTR
60 70 80 90 100
KSFWKAAVAE LLGYIRGYDN AADFRQLGTK TWDANANLNQ AWLNNPYRKG
110 120 130 140 150
EDDMGRVYGV QGRAWAKPDG GHIDQLKKIV DDLSRGVDDR GEILNFYNPG
160 170 180 190 200
EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVFLA
210 220 230 240 250
LMAQITGKKP GLAYHKIVNA HIYQDQLELM RDVQLKREPF PAPQFHINPK
260 270 280
IKTLQDLETW VTLDDFDVTG YQFHDPIQYP FSV
Length:283
Mass (Da):32,573
Last modified:August 1, 1998 - v1
Checksum:iD50B760285FDE97E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21R → K in CAA07073 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti187 – 1871G → W in inactive enzyme. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17135 Genomic DNA. Translation: CAA76645.1.
AJ006514 Genomic DNA. Translation: CAA07073.1.
AY143429 Genomic DNA. Translation: AAN16904.1.
AE003852 Genomic DNA. Translation: AAF93840.1.
PIRiC82295.
RefSeqiNP_230324.1. NC_002505.1.
WP_001250353.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF93840; AAF93840; VC_0675.
GeneIDi2615464.
KEGGivch:VC0675.
PATRICi20080467. VBIVibCho83274_0647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17135 Genomic DNA. Translation: CAA76645.1.
AJ006514 Genomic DNA. Translation: CAA07073.1.
AY143429 Genomic DNA. Translation: AAN16904.1.
AE003852 Genomic DNA. Translation: AAF93840.1.
PIRiC82295.
RefSeqiNP_230324.1. NC_002505.1.
WP_001250353.1. NC_002505.1.

3D structure databases

ProteinModelPortaliO66108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC0675.

Protocols and materials databases

DNASUi2615464.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF93840; AAF93840; VC_0675.
GeneIDi2615464.
KEGGivch:VC0675.
PATRICi20080467. VBIVibCho83274_0647.

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciVCHO:VC0675-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Valle E., Silva A., Benitez J.A.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: El Tor C7258 / Serotype O1.
  2. "Characterisation of the lgt/thyA locus from Vibrio cholerae."
    Carlin N.I.A., Nilsson A., Todorovic M., Holmgren J., Lebens M.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JS1569.
  3. Xia X.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT TRP-187.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiTYSY_VIBCH
AccessioniPrimary (citable) accession number: O66108
Secondary accession number(s): O87078, Q8GKX5, Q9JQ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.