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O66037

- PHYT_BACSD

UniProt

O66037 - PHYT_BACSD

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Protein
3-phytase
Gene
phy
Organism
Bacillus sp. (strain DS11)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.

GO - Molecular functioni

  1. 3-phytase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
3-phytase (EC:3.1.3.8)
Alternative name(s):
Myo-inositol-hexaphosphate 3-phosphohydrolase
Phytate 3-phosphatase
Gene namesi
Name:phy
OrganismiBacillus sp. (strain DS11)
Taxonomic identifieri86035 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Propeptidei27 – 304
PRO_0000022058
Chaini31 – 3833533-phytase
PRO_0000022059Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 384
Beta strandi41 – 433
Beta strandi54 – 607
Helixi66 – 683
Beta strandi70 – 756
Beta strandi81 – 844
Beta strandi89 – 924
Beta strandi98 – 10912
Beta strandi112 – 12110
Turni124 – 1263
Beta strandi128 – 1358
Turni136 – 1394
Beta strandi140 – 1434
Beta strandi147 – 1493
Beta strandi154 – 1574
Beta strandi161 – 1655
Turni167 – 1693
Beta strandi172 – 1776
Beta strandi179 – 19012
Beta strandi194 – 20512
Beta strandi210 – 2167
Turni217 – 2204
Beta strandi221 – 2266
Turni227 – 2293
Beta strandi230 – 2378
Helixi238 – 2403
Beta strandi245 – 2495
Beta strandi251 – 2544
Beta strandi259 – 2668
Helixi268 – 2703
Beta strandi272 – 2787
Helixi279 – 2813
Beta strandi283 – 2908
Beta strandi295 – 3017
Beta strandi305 – 3073
Beta strandi316 – 3194
Beta strandi324 – 3263
Beta strandi331 – 3388
Beta strandi344 – 3463
Beta strandi349 – 3546
Helixi356 – 3594
Helixi360 – 3623
Helixi374 – 3763

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CVMX-ray2.40A29-381[»]
1H6LX-ray1.80A29-381[»]
1POOX-ray2.10A29-383[»]
1QLGX-ray2.20A29-381[»]
2POOX-ray2.05A29-383[»]
ProteinModelPortaliO66037.
SMRiO66037. Positions 29-381.

Miscellaneous databases

EvolutionaryTraceiO66037.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 362332BPP
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.20. 1 hit.
InterProiIPR003431. b_Phytase.
[Graphical view]
PfamiPF02333. Phytase. 1 hit.
[Graphical view]
PROSITEiPS51662. BP_PHYTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O66037-1 [UniParc]FASTAAdd to Basket

« Hide

MNHSKTLLLT AAAGLMLTCG AVSSQAKHKL SDPYHFTVNA AAETEPVDTA    50
GDAADDPAIW LDPKNPQNSK LITTNKKSGL AVYSLEGKML HSYHTGKLNN 100
VDIRYDFPLN GKKVDIAAAS NRSEGKNTIE IYAIDGKNGT LQSITDPNRP 150
IASAIDEVYG FSLYHSQKTG KYYAMVTGKE GEFEQYELNA DKNGYISGKK 200
VRAFKMNSQT EGMAADDEYG SLYIAEEDEA IWKFSAEPDG GSNGTVIDRA 250
DGRHLTPDIE GLTIYYAADG KGYLLASSQG NSSYAIYERQ GQNKYVADFQ 300
ITDGPETDGT SDTDGIDVLG FGLGPEYPFG LFVAQDGENI DHGQKANQNF 350
KMVPWERIAD KIGFHPQVNK QVDPRKMTDR SGK 383
Length:383
Mass (Da):41,802
Last modified:August 1, 1998 - v1
Checksum:iDCB65188F7B61C8C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85968 Genomic DNA. Translation: AAC38573.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85968 Genomic DNA. Translation: AAC38573.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CVM X-ray 2.40 A 29-381 [» ]
1H6L X-ray 1.80 A 29-381 [» ]
1POO X-ray 2.10 A 29-383 [» ]
1QLG X-ray 2.20 A 29-381 [» ]
2POO X-ray 2.05 A 29-383 [» ]
ProteinModelPortali O66037.
SMRi O66037. Positions 29-381.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei O66037.

Family and domain databases

Gene3Di 2.120.10.20. 1 hit.
InterProi IPR003431. b_Phytase.
[Graphical view ]
Pfami PF02333. Phytase. 1 hit.
[Graphical view ]
PROSITEi PS51662. BP_PHYTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli."
    Kim Y.-O., Lee J.-K., Kim H.-K., Yu J.-H., Oh T.-K.
    FEMS Microbiol. Lett. 162:185-191(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-44 AND 214-223.
  2. "Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens strain."
    Ha N.-C., Kim Y.-O., Oh T.-K., Oh B.-H.
    Acta Crystallogr. D 55:691-693(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).
  3. "Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states."
    Ha N.-C., Oh B.-C., Shin S., Kim H.-J., Oh T.-K., Kim Y.-O., Choi K.Y., Oh B.-H.
    Nat. Struct. Biol. 7:147-153(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiPHYT_BACSD
AccessioniPrimary (citable) accession number: O66037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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