O66037 (PHYT_BACSD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phytase
EC=3.1.3.8

Alternative name(s):
Myo-inositol-hexaphosphate 3-phosphohydrolase
Phytate 3-phosphatase

Gene names

Name:

phy

Organism

Bacillus sp. (strain DS11)

Taxonomic identifier

86035 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.
Subcellular location	Secreted.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phytase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Propeptide

27 – 30

4

PRO_0000022058

Chain

31 – 383

353

3-phytase

PRO_0000022059

Secondary structure

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383

Helix Strand Turn

Details...

Beta strand

35 – 38

4

Beta strand

41 – 43

3

Beta strand

54 – 60

7

Helix

66 – 68

3

Beta strand

70 – 75

6

Beta strand

81 – 84

4

Beta strand

89 – 92

4

Beta strand

98 – 109

12

Beta strand

112 – 121

10

Helix

123 – 125

3

Beta strand

128 – 134

7

Turn

136 – 138

3

Beta strand

141 – 143

3

Beta strand

147 – 149

3

Beta strand

154 – 165

12

Turn

167 – 169

3

Beta strand

172 – 178

7

Beta strand

181 – 190

10

Beta strand

194 – 205

12

Beta strand

210 – 216

7

Turn

217 – 220

4

Beta strand

221 – 226

6

Turn

227 – 229

3

Beta strand

230 – 235

6

Helix

238 – 240

3

Beta strand

245 – 249

5

Beta strand

251 – 254

4

Beta strand

259 – 266

8

Beta strand

272 – 278

7

Turn

279 – 282

4

Beta strand

283 – 290

8

Beta strand

295 – 301

7

Beta strand

305 – 307

3

Beta strand

316 – 319

4

Beta strand

324 – 326

3

Beta strand

331 – 338

8

Beta strand

349 – 354

6

Helix

355 – 359

5

Helix

360 – 362

3

Sequences

	Sequence		Length	Mass (Da)
	O66037 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: DCB65188F7B61C8C	FASTA	383	41,802
	10 20 30 40 50 60 MNHSKTLLLT AAAGLMLTCG AVSSQAKHKL SDPYHFTVNA AAETEPVDTA GDAADDPAIW 70 80 90 100 110 120 LDPKNPQNSK LITTNKKSGL AVYSLEGKML HSYHTGKLNN VDIRYDFPLN GKKVDIAAAS 130 140 150 160 170 180 NRSEGKNTIE IYAIDGKNGT LQSITDPNRP IASAIDEVYG FSLYHSQKTG KYYAMVTGKE 190 200 210 220 230 240 GEFEQYELNA DKNGYISGKK VRAFKMNSQT EGMAADDEYG SLYIAEEDEA IWKFSAEPDG 250 260 270 280 290 300 GSNGTVIDRA DGRHLTPDIE GLTIYYAADG KGYLLASSQG NSSYAIYERQ GQNKYVADFQ 310 320 330 340 350 360 ITDGPETDGT SDTDGIDVLG FGLGPEYPFG LFVAQDGENI DHGQKANQNF KMVPWERIAD 370 380 KIGFHPQVNK QVDPRKMTDR SGK

References

[1]	"Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli." Kim Y.-O., Lee J.-K., Kim H.-K., Yu J.-H., Oh T.-K. FEMS Microbiol. Lett. 162:185-191(1998) [PubMed: 9595681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-44 AND 214-223.
[2]	"Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens strain." Ha N.-C., Kim Y.-O., Oh T.-K., Oh B.-H. Acta Crystallogr. D 55:691-693(1999) [PubMed: 10089471] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).
[3]	"Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states." Ha N.-C., Oh B.-C., Shin S., Kim H.-J., Oh T.-K., Kim Y.-O., Choi K.Y., Oh B.-H. Nat. Struct. Biol. 7:147-153(2000) [PubMed: 10655618] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U85968 Genomic DNA. Translation: AAC38573.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CVM	X-ray	2.40	A	29-381	[»]
1H6L	X-ray	1.80	A	29-381	[»]
1POO	X-ray	2.10	A	29-383	[»]
1QLG	X-ray	2.20	A	29-383	[»]
2POO	X-ray	2.05	A	29-383	[»]

ProteinModelPortal

O66037.

SMR

O66037. Positions 29-381.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR003431. Phytase.
[Graphical view]

Gene3D

G3DSA:2.120.10.20. Phytase. 1 hit.

Pfam

PF02333. Phytase. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PHYT_BACSD

Accession

Primary (citable) accession number: O66037

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 30, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program