Reviewed, UniProtKB/Swiss-Prot O65989 (PTMCB_CLOAB)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICB component
Alternative name(s):
    EIICB-Mtl
      Short name=EII-Mtl
Including the following 2 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component

Gene names

Name:	mtlA
Ordered Locus Names:	CA_C0154

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	PTS system mannitol-specific EIICB component		PRO_0000186618
Regions
	Transmembrane	28 – 48	21	Potential
	Transmembrane	93 – 113	21	Potential
	Transmembrane	145 – 165	21	Potential
	Transmembrane	171 – 191	21	Potential
	Transmembrane	223 – 243	21	Potential
	Transmembrane	255 – 275	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	298 – 318	21	Potential
	Transmembrane	323 – 343	21	Potential
	Domain	22 – 357	336	PTS EIIC type-2
	Domain	392 – 481	90	PTS EIIB type-2
Sites
	Active site	398	1	Phosphocysteine intermediate; for EIIB activity By similarity
Experimental info
	Sequence conflict	414	1	K → E in AAC12848. Ref.1
	Sequence conflict	438 – 439	2	AD → RS in AAC12848. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O65989-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 9A682C351E361FFA	FASTA	481	50,798
	10 20 30 40 50 60 METYKDSTQV SKSSLKKKIQ GFGGFLSGMV MPNIGAFIAW GLITALFIKT GWLPNDNLSK 70 80 90 100 110 120 LVDPMIHYML PMLIGYQGGK LVYDTRGGVV GAIATMGMIV GASIPMFLGG MIIGPLGGYV 130 140 150 160 170 180 IKKFDKAIEN KIPTGFEMLV NNFSAGILGA ALAIISYVAV GPVVAGASTG LGSIALAITN 190 200 210 220 230 240 QGLLPLIAVV VEPAKILFLN NAINHGVFSP LGIEQVQHLG KSVFFLLEAD PGPGLGILLA 250 260 270 280 290 300 YSLYGKGSAK NSAPGAVIIH FLGGIHEIYF PYVLMKPFLL LAVIAGGICA DLTFVLLKAG 310 320 330 340 350 360 LVAAASPGSI IAILAMSPKG GQLPVLAGVA VGAIVSFVVA SIILKGSKEK SKDNFEEAQN 370 380 390 400 410 420 KMKEMKKESK NQTTANSENV KNNDELVSSD IKLIVFACDA GMGSSAMGES ILKKELKNAN 430 440 450 460 470 480 IDGIKVQHYS VDSIPKEADV VFVQENLSER ARKSAPDANI VTIKNFLDRS TYEGFMKKIK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator." Behrens S., Mitchell W.J., Bahl H. Microbiology 147:75-86(2001) [PubMed: 11160802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U53868 Genomic DNA. Translation: AAC12848.1. AE001437 Genomic DNA. Translation: AAK78138.1.
PIR	G96918.
RefSeq	NP_346798.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1116337.
GenomeReviews	Gene locus CA_C0154 in contig AE001437_GR.
KEGG	cac:CAC0154.
NMPDR	fig|272562.1.peg.327.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O65989.
OMA	ITHKDLT.
Enzyme and pathway databases
BioCyc	CACE272562:CAC0154-MON.
BRENDA	2.7.1.69. 2866.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003501. PTS_IIB_lac. IPR004718. PTS_IIC_mtl. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTMCB_CLOAB

Accession

Primary (citable) accession number: O65989

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 29, 2001
Last modified:	November 3, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)