O65896 (O65896_ARATH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Cytidine deaminase PIRNR PIRNR006334
EC=3.5.4.5 PIRNR PIRNR006334

Gene names

Name:	cda1 EMBL CAA06671.1
Synonyms:	CDA1 EMBL AEC06896.1, CDD EMBL CAA06460.1
Ordered Locus Names:	At2g19570 TAIR At2g19570 EMBL AAM91569.1
ORF Names:	AT2G19570 EMBL AEC06896.1

Organism

Arabidopsis thaliana (Mouse-ear cress) EMBL CAA06671.1

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Cytidine + H2O = uridine + NH3. PIRNR PIRNR006334
Cofactor	Binds 1 zinc ion By similarity. PIRNR PIRNR006334 PIRSR PIRSR006334-3
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family. PIRNR PIRNR006334

Ontologies

Keywords
Ligand	Metal-binding PIRNR PIRNR006334 PIRSR PIRSR006334-3 Zinc PIRNR PIRNR006334 PIRSR PIRSR006334-3
Molecular function	Hydrolase PIRNR PIRNR006334 EMBL CAA06671.1
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytosol Inferred from direct assay. Source: TAIR
Molecular function	cytidine deaminase activity Inferred from direct assay Ref.4. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	64 – 66	3	Substrate binding By similarity PIRSR PIRSR006334-2
Sites
	Active site	79	1	Proton donor By similarity PIRSR PIRSR006334-1
	Metal binding	77	1	Zinc; catalytic By similarity PIRSR PIRSR006334-3
	Metal binding	104	1	Zinc; catalytic By similarity PIRSR PIRSR006334-3
	Metal binding	107	1	Zinc; catalytic By similarity PIRSR PIRSR006334-3

Sequences

	Sequence		Length	Mass (Da)
	O65896 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 91C17F09AA70CBB2	FASTA	301	32,582
	10 20 30 40 50 60 MDKPSFVIQS KEAESAAKQL GVSVIQLLPS LVKPAQSYAR TPISKFNVAV VGLGSSGRIF 70 80 90 100 110 120 LGVNVEFPNL PLHHSIHAEQ FLVTNLTLNG ERHLNFFAVS AAPCGHCRQF LQEIRDAPEI 130 140 150 160 170 180 KILITDPNNS ADSDSAADSD GFLRLGSFLP HRFGPDDLLG KDHPLLLESH DNHLKISDLD 190 200 210 220 230 240 SICNGNTDSS ADLKQTALAA ANRSYAPYSL CPSGVSLVDC DGKVYRGWYM ESAAYNPSMG 250 260 270 280 290 300 PVQAALVDYV ANGGGGGYER IVGAVLVEKE DAVVRQEHTA RLLLETISPK CEFKVFHCYE A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"A prokaryotic-type cytidine deaminase from Arabidopsis thaliana." Faivre-Nitschke E.S., Grienenberger J.M., Gualberto J.M. Eur. J. Biochem. 263:896-903(1999) [PubMed: 10469156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations." Elledge S.J., Mulligan J.T., Ramer S.W., Spottswood M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 88:1731-1735(1991) [PubMed: 1848010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Cloning, expression, and purification of cytidine deaminase from Arabidopsis thaliana." Vincenzetti S., Cambi A., Neuhard J., Schnorr K., Grelloni M., Vita A. Protein Expr. Purif. 15:8-15(1999) [PubMed: 10024464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	TAIR Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[6]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[7]	Town C.D., Kaul S. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[8]	Lin X., Kaul S., Shea T.P., Fujii C.Y., Shen M., VanAken S.E., Barnstead M.E., Mason T.M., Bowman C.L., Ronning C.M., Benito M.-I., Carrera A.J., Creasy T.H., Buell C.R., Town C.D., Nierman W.C., Fraser C.M., Venter J.C. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[9]	Schnorr K.M. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[10]	Tripp M., Southwick A., Karlin-Neumann G., Nguyen M., Miranda M., Palm C.J., Bowser L., Jones T., Banh J., Carninci P., Chen H., Cheuk R., Chung M.K., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Kim C. , Lin J., Liu S.X., Narusaka M., Pham P.K., Sakano H., Sakurai T., Satou M., Seki M., Shinn P., Yamada K., Shinozaki K., Ecker J., Theologis A., Davis R.W. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[11]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R., Shinn P., Bowser L., Carninci P., Dale J.M., Hayashizaki Y., Hsuan V.W., Ishida J., Jones T., Kamiya A., Karlin-Neumann G., Kawai J., Lam B., Lin J., Miranda M., Narusaka M. , Nguyen M., Onodera C.S., Palm C.J., Quach H.L., Sakurai T., Satou M., Seki M., Southwick A., Toriumi M., Wong C., Wu H.C., Yamada K., Yu G., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[12]	"Arabidopsis thaliana cytidine deaminase 1 shows more similarity to prokaryotic enzymes than to eukaryotic enzymes." Kafer C.W., Thornburg R.W. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005917 Genomic DNA. Translation: AAD10156.1. AF134487 mRNA. Translation: AAF03358.1. AY128366 mRNA. Translation: AAM91569.1. BT008708 mRNA. Translation: AAP42721.1. CP002685 Genomic DNA. Translation: AEC06896.1. AJ005261 mRNA. Translation: CAA06460.1. AJ005687 Genomic DNA. Translation: CAA06671.1.
IPI	IPI00547274.
PIR	T51733.
RefSeq	NP_179547.1. NM_127515.4.
UniGene	At.25410. At.69521.
3D structure databases
HSSP	HSSP built from PDB template 1CTT based on UniProtKB P13652.
ProteinModelPortal	O65896.
SMR	O65896. Positions 2-290.
ModBase	Search...
Protein-protein interaction databases
IntAct	O65896. 1 interaction.
STRING	O65896.
Proteomic databases
PRIDE	O65896.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G19570.1; AT2G19570.1; AT2G19570.
GeneID	816476.
KEGG	ath:AT2G19570.
Organism-specific databases
TAIR	At2g19570.
Phylogenomic databases
eggNOG	KOG0833.
InParanoid	O65896.
OMA	NRSHAPY.
PhylomeDB	O65896.
ProtClustDB	PLN02402.
Gene expression databases
ArrayExpress	O65896.
Genevestigator	O65896.
Family and domain databases
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR002125. CMP_dCMP_Zn-bd. IPR013171. Cyd/dCyd_deaminase_Zn-bd. IPR006263. Cyt_deam_dimer. IPR016193. Cytidine_deaminase-like. [Graphical view]
KO	K01489.
Pfam	PF00383. dCMP_cyt_deam_1. 1 hit. PF08211. dCMP_cyt_deam_2. 1 hit. [Graphical view]
PIRSF	PIRSF006334. Cdd_plus_pseudo. 1 hit.
SUPFAM	SSF53927. Cytidine_deaminase-like. 2 hits.
TIGRFAMs	TIGR01355. Cyt_deam_dimer. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O65896_ARATH

Accession

Primary (citable) accession number: O65896

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)