O65857 (GSTF1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione S-transferase GSTF1
EC=2.5.1.18

Alternative name(s):
GST-I

Gene names

Name:	GSTF1
Synonyms:	RGST I
Ordered Locus Names:	Os01g0371200, LOC_Os01g27360
ORF Names:	B1039D07.2, P0493G01.33

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Tissue specificity	Constitutively expressed in roots. Ref.5
Induction	By the herbicide safener fenclorim. Ref.5
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Probable glutathione S-transferase GSTF1		PRO_0000185844
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	91 – 219	129	GST C-terminal
	Region	41 – 42	2	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione By similarity
Experimental info
	Sequence conflict	193	1	H → Q in CAA05354. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O65857 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: FEB7B2BCC8AABF11	FASTA	219	24,924
	10 20 30 40 50 60 MTPVKVFGPA QSTNVARVLL CLEEVGAEYE VVNVDFTVME HKSPEHLKRN PFGQIPAFQD 70 80 90 100 110 120 GDLYLFESRA IGKYILRKYK TREADLLREG NLREAAMVDV WTEVETHQYN SAISPIVYEC 130 140 150 160 170 180 IINPAMRGIP TNQKVVDESA EKLKKVLEVY EARLSQSTYL AGDFVSFADL NHFPYTFYFM 190 200 210 GTPYASLFDS YPHVKAWWER LMARPSVKKL AAVMAPQGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Plant genes involved in defense against pathogens." Chang H.-S., Chen W., Cooper B., Glazebrook J., Goff S.A., Hou Y.-M., Katagiri F., Quan S., Tao Y., Whitham S., Xie Z., Zhu T., Zou G. Patent number WO03000898, 03-JAN-2003 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Nucleotide sequence of a cDNA encoding glutathione S-transferase from rice (Oryza sativa)." Wu J., Cramer C., Hatzios K.K. Plant Gene Register PGR98-120 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-198. Strain: cv. Lemont.
[5]	"Characterization of two cDNAs encoding glutathione S-transferases in rice and induction of their transcripts by the herbicide safener fenclorim." Wu J., Cramer C., Hatzios K.K. Physiol. Plantarum 105:102-108(1999) Cited for: CHARACTERIZATION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Lemont.
[6]	"Organisation and structural evolution of the rice glutathione S-transferase gene family." Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C. Mol. Genet. Genomics 271:511-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AX654621 Unassigned DNA. No translation available. AP002914 Genomic DNA. Translation: BAB39939.1. AP003198 Genomic DNA. Translation: BAB64040.1. AK058900 mRNA. No translation available. AJ002380 mRNA. Translation: CAA05354.1.
PIR	T03987.
RefSeq	NP_001043059.1. NM_001049594.1.
UniGene	Os.25651.
3D structure databases
ProteinModelPortal	O65857.
SMR	O65857. Positions 3-218.
ModBase	Search...
Proteomic databases
PaxDb	O65857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g27360.1; LOC_Os01g27360.1; LOC_Os01g27360.
GeneID	4325710.
KEGG	osa:4325710.
Organism-specific databases
Gramene	O65857.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125746.
OMA	ILWYLAE.
ProtClustDB	CLSN2691436.
Gene expression databases
ArrayExpress	O65857.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF1_ORYSJ

Accession

Primary (citable) accession number: O65857
Secondary accession number(s): Q9AS49

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program