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O65796

- HEM13_HORVU

UniProt

O65796 - HEM13_HORVU

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Protein

Glutamyl-tRNA reductase 3, chloroplastic

Gene
HEMA3
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity.UniRule annotation

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311Nucleophile By similarity
Sitei180 – 1801Important for activity By similarity
Binding sitei190 – 1901Substrate By similarity
Binding sitei201 – 2011Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776NADP By similarity

GO - Molecular functioni

  1. glutamyl-tRNA reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-KW
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductase 3, chloroplastic (EC:1.2.1.70)
Short name:
GluTR
Gene namesi
Name:HEMA3
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiO65796.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 535Glutamyl-tRNA reductase 3, chloroplasticUniRule annotationPRO_0000013312
Transit peptidei1 – ?Chloroplast Reviewed prediction

Expressioni

Tissue specificityi

Primarily expressed in roots.

Gene expression databases

GenevestigatoriO65796.

Structurei

3D structure databases

ProteinModelPortaliO65796.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1333Substrate binding By similarity
Regioni195 – 1973Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
PIRSFiPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMiSSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65796-1 [UniParc]FASTAAdd to Basket

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MASTSTASAT AMAGAFAAAG VNKPRGSAAC PRVPAGGRQR LSCVVRCDAG    50
PGVPAQMAAM AASVAALEQF KISADRYMKE KSSIAVIGLS IHTAPVEMRE 100
KLAVAEELWP RAVAELTNLN HIEKAAVLSP CNRMEIYVVA LSWNRGIREI 150
VDWMSMKSGI PAVELREHLF MFRDSDATRH LFEVSSGLDS LVLGEGQILA 200
QVKQVVRSGQ NSGGLGKNID RMFKDAITAG KRVRSETNIS CGAVSVSSAA 250
VELALMKLPK SECLSARMLL IGAGKMGRLV AKHLAAKGCK KVVIVNRSVE 300
RVDAIREEMQ GIEIVYRSLT EMYEAAADAD VVFTSTSSES PLFTKEHAEA 350
LPPVSGALGG VRLFVDISVP RNVSACVSDV GHARVYNVDD LKEVVEANKE 400
DRLRKAMEAQ TIISEELKRF EAWRDSMETV PTIKKLRSYA DRVRASELDK 450
CLQKIGEDAL TKKMRRSIEQ LSTGIVNRLL HGPLQHLRCD GTDNRTLDET 500
LENMHALNRM FGLDTEKAVM EQKIKTKVEK QKTQN 535
Length:535
Mass (Da):58,419
Last modified:August 1, 1998 - v1
Checksum:iB2830889AE6A3224
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88383 mRNA. Translation: BAA25168.1.
PIRiT04402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88383 mRNA. Translation: BAA25168.1 .
PIRi T04402.

3D structure databases

ProteinModelPortali O65796.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei O65796.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00316 .

Gene expression databases

Genevestigatori O65796.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00087. Glu_tRNA_reductase.
InterProi IPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view ]
Pfami PF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMi SSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsi TIGR01035. hemA. 1 hit.
PROSITEi PS00747. GLUTR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The third member of the hemA gene family encoding glutamyl-tRNA reductase is primarily expressed in roots in Hordeum vulgare."
    Tanaka R., Yoshida K., Nakayashiki T., Tsuji H., Inokuchi H., Okada K., Tanaka A.
    Photosyn. Res. 53:161-171(1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Bonus.
    Tissue: Root.

Entry informationi

Entry nameiHEM13_HORVU
AccessioniPrimary (citable) accession number: O65796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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