Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O65796

- HEM13_HORVU

UniProt

O65796 - HEM13_HORVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamyl-tRNA reductase 3, chloroplastic

Gene

HEMA3

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311NucleophileBy similarity
Sitei180 – 1801Important for activityBy similarity
Binding sitei190 – 1901SubstrateBy similarity
Binding sitei201 – 2011SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776NADPBy similarity

GO - Molecular functioni

  1. glutamyl-tRNA reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-KW
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductase 3, chloroplastic (EC:1.2.1.70)
Short name:
GluTR
Gene namesi
Name:HEMA3
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiO65796.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 535Glutamyl-tRNA reductase 3, chloroplasticPRO_0000013312
Transit peptidei1 – ?ChloroplastSequence Analysis

Expressioni

Tissue specificityi

Primarily expressed in roots.

Gene expression databases

GenevestigatoriO65796.

Structurei

3D structure databases

ProteinModelPortaliO65796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1333Substrate bindingBy similarity
Regioni195 – 1973Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
PIRSFiPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMiSSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65796-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASTSTASAT AMAGAFAAAG VNKPRGSAAC PRVPAGGRQR LSCVVRCDAG
60 70 80 90 100
PGVPAQMAAM AASVAALEQF KISADRYMKE KSSIAVIGLS IHTAPVEMRE
110 120 130 140 150
KLAVAEELWP RAVAELTNLN HIEKAAVLSP CNRMEIYVVA LSWNRGIREI
160 170 180 190 200
VDWMSMKSGI PAVELREHLF MFRDSDATRH LFEVSSGLDS LVLGEGQILA
210 220 230 240 250
QVKQVVRSGQ NSGGLGKNID RMFKDAITAG KRVRSETNIS CGAVSVSSAA
260 270 280 290 300
VELALMKLPK SECLSARMLL IGAGKMGRLV AKHLAAKGCK KVVIVNRSVE
310 320 330 340 350
RVDAIREEMQ GIEIVYRSLT EMYEAAADAD VVFTSTSSES PLFTKEHAEA
360 370 380 390 400
LPPVSGALGG VRLFVDISVP RNVSACVSDV GHARVYNVDD LKEVVEANKE
410 420 430 440 450
DRLRKAMEAQ TIISEELKRF EAWRDSMETV PTIKKLRSYA DRVRASELDK
460 470 480 490 500
CLQKIGEDAL TKKMRRSIEQ LSTGIVNRLL HGPLQHLRCD GTDNRTLDET
510 520 530
LENMHALNRM FGLDTEKAVM EQKIKTKVEK QKTQN
Length:535
Mass (Da):58,419
Last modified:August 1, 1998 - v1
Checksum:iB2830889AE6A3224
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88383 mRNA. Translation: BAA25168.1.
PIRiT04402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88383 mRNA. Translation: BAA25168.1 .
PIRi T04402.

3D structure databases

ProteinModelPortali O65796.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei O65796.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00316 .

Gene expression databases

Genevestigatori O65796.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00087. Glu_tRNA_reductase.
InterProi IPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view ]
Pfami PF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMi SSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsi TIGR01035. hemA. 1 hit.
PROSITEi PS00747. GLUTR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The third member of the hemA gene family encoding glutamyl-tRNA reductase is primarily expressed in roots in Hordeum vulgare."
    Tanaka R., Yoshida K., Nakayashiki T., Tsuji H., Inokuchi H., Okada K., Tanaka A.
    Photosyn. Res. 53:161-171(1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Bonus.
    Tissue: Root.

Entry informationi

Entry nameiHEM13_HORVU
AccessioniPrimary (citable) accession number: O65796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3