Reviewed, UniProtKB/Swiss-Prot O65796 (HEM13_HORVU)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA reductase 3, chloroplastic
      Short name=GluTR
    EC=1.2.1.70

Gene names

Name:

HEMA3

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity.
Catalytic activity	L-glutamate 1-semialdehyde + NADP(+) + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Primarily expressed in roots.
Miscellaneous	During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity.
Sequence similarities	Belongs to the glutamyl-tRNA reductase family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamyl-tRNA reductase activity Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 535		Glutamyl-tRNA reductase 3, chloroplastic		PRO_0000013312
Regions
	Nucleotide binding	272 – 277	6	NADP By similarity
	Region	131 – 133	3	Substrate binding By similarity
	Region	195 – 197	3	Substrate binding By similarity
Sites
	Active site	131	1	Nucleophile By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	201	1	Substrate By similarity
	Site	180	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O65796-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B2830889AE6A3224	FASTA	535	58,419
	10 20 30 40 50 60 MASTSTASAT AMAGAFAAAG VNKPRGSAAC PRVPAGGRQR LSCVVRCDAG PGVPAQMAAM 70 80 90 100 110 120 AASVAALEQF KISADRYMKE KSSIAVIGLS IHTAPVEMRE KLAVAEELWP RAVAELTNLN 130 140 150 160 170 180 HIEKAAVLSP CNRMEIYVVA LSWNRGIREI VDWMSMKSGI PAVELREHLF MFRDSDATRH 190 200 210 220 230 240 LFEVSSGLDS LVLGEGQILA QVKQVVRSGQ NSGGLGKNID RMFKDAITAG KRVRSETNIS 250 260 270 280 290 300 CGAVSVSSAA VELALMKLPK SECLSARMLL IGAGKMGRLV AKHLAAKGCK KVVIVNRSVE 310 320 330 340 350 360 RVDAIREEMQ GIEIVYRSLT EMYEAAADAD VVFTSTSSES PLFTKEHAEA LPPVSGALGG 370 380 390 400 410 420 VRLFVDISVP RNVSACVSDV GHARVYNVDD LKEVVEANKE DRLRKAMEAQ TIISEELKRF 430 440 450 460 470 480 EAWRDSMETV PTIKKLRSYA DRVRASELDK CLQKIGEDAL TKKMRRSIEQ LSTGIVNRLL 490 500 510 520 530 HGPLQHLRCD GTDNRTLDET LENMHALNRM FGLDTEKAVM EQKIKTKVEK QKTQN

References

[1]

"The third member of the hemA gene family encoding glutamyl-tRNA reductase is primarily expressed in roots in Hordeum vulgare." Tanaka R., Yoshida K., Nakayashiki T., Tsuji H., Inokuchi H., Okada K., Tanaka A. Photosyn. Res. 53:161-171(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bonus. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D88383 mRNA. Translation: BAA25168.1.
PIR	T04402.
3D structure databases
HSSP	HSSP built from PDB template 1B29 based on UniProtKB Q42843.
ModBase	Search...
Organism-specific databases
Gramene	O65796.
Family and domain databases
InterPro	IPR000343. 4pyrrol_synth_GluRdtase. IPR015896. 4pyrrol_synth_GluRdtase_C. IPR015895. 4pyrrol_synth_GluRdtase_N. IPR016040. NAD(P)-bd. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00745. GlutR_dimer. 1 hit. PF05201. GlutR_N. 1 hit. PF01488. Shikimate_DH. 1 hit. [Graphical view]
PIRSF	PIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
TIGRFAMs	TIGR01035. hemA. 1 hit.
PROSITE	PS00747. GLUTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM13_HORVU

Accession

Primary (citable) accession number: O65796

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)