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Protein

Cytochrome P450 81F1

Gene

CYP81F1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in indole glucosinolate biosynthesis. Catalyzes hydroxylation reactions of the glucosinolate indole ring. Converts indol-3-yl-methylglucosinolate (I3M) to 4-hydroxy-indol-3-yl-methylglucosinolate (4OH-I3M) and/or 1-hydroxy-indol-3-yl-methylglucosinolate (1OH-I3M) intermediates. These hydroxy intermediates are converted to 4-methoxy-indol-3-yl-methylglucosinolate (4MO-I3M) and 1-methoxy-indol-3-yl-methylglucosinolate (1MO-I3M) by indole glucosinolate methyltransferase 1 and 2 (IGMT1 and IGMT2).1 Publication

Cofactori

hemeBy similarity

Pathwayi: Secondary metabolite biosynthesis

This protein is involved in Secondary metabolite biosynthesis.Curated
View all proteins of this organism that are known to be involved in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi438Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G37430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 81F1Curated (EC:1.14.-.-Curated)
Gene namesi
Name:CYP81F11 Publication
Synonyms:CYP91A2
Ordered Locus Names:At4g37430
ORF Names:F6G17.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37430.
TAIRilocus:2126402. AT4G37430.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: GO_Central

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521611 – 500Cytochrome P450 81F1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki248Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO65790.

PTM databases

iPTMnetiO65790.

Expressioni

Gene expression databases

ExpressionAtlasiO65790. baseline and differential.
GenevisibleiO65790. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G37430.1.

Structurei

3D structure databases

ProteinModelPortaliO65790.
SMRiO65790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218627.
InParanoidiO65790.
KOiK00517.
OMAiANHSADY.
OrthoDBiEOG09360EOO.
PhylomeDBiO65790.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

O65790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYFILLPLL FLVISYKFLY SKTQRFNLPP GPPSRPFVGH LHLMKPPIHR
60 70 80 90 100
LLQRYSNQYG PIFSLRFGSR RVVVITSPSL AQESFTGQND IVLSSRPLQL
110 120 130 140 150
TAKYVAYNHT TVGTAPYGDH WRNLRRICSQ EILSSHRLIN FQHIRKDEIL
160 170 180 190 200
RMLTRLSRYT QTSNESNDFT HIELEPLLSD LTFNNIVRMV TGKRYYGDDV
210 220 230 240 250
NNKEEAELFK KLVYDIAMYS GANHSADYLP ILKLFGNKFE KEVKAIGKSM
260 270 280 290 300
DDILQRLLDE CRRDKEGNTM VNHLISLQQQ QPEYYTDVII KGLMMSMMLA
310 320 330 340 350
GTETSAVTLE WAMANLLRNP EVLEKARSEI DEKIGKDRLI DESDIAVLPY
360 370 380 390 400
LQNVVSETFR LFPVAPFLIP RSPTDDMKIG GYDVPRDTIV MVNAWAIHRD
410 420 430 440 450
PEIWEEPEKF NPDRYNDGCG SDYYVYKLMP FGNGRRTCPG AGLGQRIVTL
460 470 480 490 500
ALGSLIQCFE WENVKGEEMD MSESTGLGMR KMDPLRAMCR PRPIMSKLLL
Length:500
Mass (Da):57,555
Last modified:December 1, 2000 - v2
Checksum:i0FB453D2070EA2EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102A → S in AAM67324 (Ref. 5) Curated1
Sequence conflicti106A → V in BAA28539 (PubMed:9620263).Curated1
Sequence conflicti127I → M in BAA28539 (PubMed:9620263).Curated1
Sequence conflicti140N → I in BAA28539 (PubMed:9620263).Curated1
Sequence conflicti454S → T in BAA28539 (PubMed:9620263).Curated1
Sequence conflicti495M → I in AAM67324 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78607 mRNA. Translation: BAA28539.1.
AL035601 Genomic DNA. Translation: CAB38210.1.
AL161591 Genomic DNA. Translation: CAB80408.1.
CP002687 Genomic DNA. Translation: AEE86792.1.
AY039844 mRNA. Translation: AAK63948.1.
AY087256 mRNA. Translation: AAM67324.1.
PIRiT04737.
T52175.
RefSeqiNP_195459.1. NM_119906.3.
UniGeneiAt.20210.

Genome annotation databases

EnsemblPlantsiAT4G37430.1; AT4G37430.1; AT4G37430.
GeneIDi829897.
GrameneiAT4G37430.1; AT4G37430.1; AT4G37430.
KEGGiath:AT4G37430.

Similar proteinsi

Entry informationi

Entry nameiC81F1_ARATH
AccessioniPrimary (citable) accession number: O65790
Secondary accession number(s): Q7FBW2, Q8LBE7, Q9SZU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: August 30, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families