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Protein

Cytochrome P450 83B1

Gene

CYP83B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the metabolism of aromatic oximes. Catalyzes the oxime metabolizing step in indole glucosinolate biosynthesis by converting indole-3-acetaldoxime into indole-3-S-alkyl-thiohydroximate. Probably required for glucosinolate activation in response to pathogens. Functions in auxin homeostasis because indole-3-acetaldoxime also serves as a precursor for auxin biosynthesis.5 Publications

Cofactori

hemeBy similarity

Kineticsi

  1. KM=3.1 µM for indole-3-acetaldoxime2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi441 – 4411Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    GO - Biological processi

    • adventitious root development Source: TAIR
    • defense response by callose deposition in cell wall Source: TAIR
    • defense response to bacterium Source: TAIR
    • indoleacetic acid biosynthetic process Source: TAIR
    • indole glucosinolate biosynthetic process Source: TAIR
    • induced systemic resistance Source: TAIR
    • regulation of growth Source: UniProtKB-KW
    • response to red light Source: TAIR
    • shade avoidance Source: TAIR
    • tryptophan biosynthetic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Growth regulation

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G31500-MONOMER.
    MetaCyc:AT4G31500-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 83B1 (EC:1.14.-.-)
    Alternative name(s):
    Protein ALTERED TRYPTOPHAN REGULATION 4
    Protein RED ELONGATED 1
    Protein SUPERROOT 2
    Gene namesi
    Name:CYP83B1
    Synonyms:ATR4, RED1, RNT1, RUNT1, SUR2
    Ordered Locus Names:At4g31500
    ORF Names:F3L17.70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G31500.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • endoplasmic reticulum Source: TAIR
    • integral component of membrane Source: UniProtKB-KW
    • membrane Source: TAIR
    • mitochondrion Source: TAIR
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Runt phenotype (small plants with hooked leaves). Epinastic leaves and defect in root development. High levels of endogenous free auxin. Altered tryptophan genes regulation.6 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Cytochrome P450 83B1PRO_0000052167Add
    BLAST

    Proteomic databases

    PaxDbiO65782.
    PRIDEiO65782.

    Expressioni

    Inductioni

    By auxin and continuous red light.2 Publications

    Gene expression databases

    GenevisibleiO65782. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi14563. 1 interaction.
    STRINGi3702.AT4G31500.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO65782.
    SMRiO65782. Positions 50-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0156. Eukaryota.
    COG2124. LUCA.
    HOGENOMiHOG000218629.
    InParanoidiO65782.
    KOiK11818.
    OMAiIKFTHEN.
    PhylomeDBiO65782.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O65782-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDLLLIIAGL VAAAAFFFLR STTKKSLRLP PGPKGLPIIG NLHQMEKFNP
    60 70 80 90 100
    QHFLFRLSKL YGPIFTMKIG GRRLAVISSA ELAKELLKTQ DLNFTARPLL
    110 120 130 140 150
    KGQQTMSYQG RELGFGQYTA YYREMRKMCM VNLFSPNRVA SFRPVREEEC
    160 170 180 190 200
    QRMMDKIYKA ADQSGTVDLS ELLLSFTNCV VCRQAFGKRY NEYGTEMKRF
    210 220 230 240 250
    IDILYETQAL LGTLFFSDLF PYFGFLDNLT GLSARLKKAF KELDTYLQEL
    260 270 280 290 300
    LDETLDPNRP KQETESFIDL LMQIYKDQPF SIKFTHENVK AMILDIVVPG
    310 320 330 340 350
    TDTAAAVVVW AMTYLIKYPE AMKKAQDEVR SVIGDKGYVS EEDIPNLPYL
    360 370 380 390 400
    KAVIKESLRL EPVIPILLHR ETIADAKIGG YDIPAKTIIQ VNAWAVSRDT
    410 420 430 440 450
    AAWGDNPNEF IPERFMNEHK GVDFKGQDFE LLPFGSGRRM CPAMHLGIAM
    460 470 480 490
    VEIPFANLLY KFDWSLPKGI KPEDIKMDVM TGLAMHKKEH LVLAPTKHI
    Length:499
    Mass (Da):56,846
    Last modified:August 1, 1998 - v1
    Checksum:iCCA2D733E7D00D7B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D78598 mRNA. Translation: BAA28531.1.
    AL080283 Genomic DNA. Translation: CAB45909.1.
    AL161579 Genomic DNA. Translation: CAB79868.1.
    CP002687 Genomic DNA. Translation: AEE85921.1.
    BT002333 mRNA. Translation: AAN86166.1.
    AY085195 mRNA. Translation: AAM61746.1.
    PIRiT10680.
    RefSeqiNP_194878.1. NM_119299.2.
    UniGeneiAt.24671.

    Genome annotation databases

    EnsemblPlantsiAT4G31500.1; AT4G31500.1; AT4G31500.
    GeneIDi829277.
    GrameneiAT4G31500.1; AT4G31500.1; AT4G31500.
    KEGGiath:AT4G31500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D78598 mRNA. Translation: BAA28531.1.
    AL080283 Genomic DNA. Translation: CAB45909.1.
    AL161579 Genomic DNA. Translation: CAB79868.1.
    CP002687 Genomic DNA. Translation: AEE85921.1.
    BT002333 mRNA. Translation: AAN86166.1.
    AY085195 mRNA. Translation: AAM61746.1.
    PIRiT10680.
    RefSeqiNP_194878.1. NM_119299.2.
    UniGeneiAt.24671.

    3D structure databases

    ProteinModelPortaliO65782.
    SMRiO65782. Positions 50-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi14563. 1 interaction.
    STRINGi3702.AT4G31500.1.

    Proteomic databases

    PaxDbiO65782.
    PRIDEiO65782.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G31500.1; AT4G31500.1; AT4G31500.
    GeneIDi829277.
    GrameneiAT4G31500.1; AT4G31500.1; AT4G31500.
    KEGGiath:AT4G31500.

    Organism-specific databases

    TAIRiAT4G31500.

    Phylogenomic databases

    eggNOGiKOG0156. Eukaryota.
    COG2124. LUCA.
    HOGENOMiHOG000218629.
    InParanoidiO65782.
    KOiK11818.
    OMAiIKFTHEN.
    PhylomeDBiO65782.

    Enzyme and pathway databases

    BioCyciARA:AT4G31500-MONOMER.
    MetaCyc:AT4G31500-MONOMER.

    Miscellaneous databases

    PROiO65782.

    Gene expression databases

    GenevisibleiO65782. AT.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs, differential expression, and RFLP mapping of multiple cytochromes P450."
      Mizutani M., Ward E., Ohta D.
      Plant Mol. Biol. 37:39-52(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Seedling.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Sur2 mutations of Arabidopsis thaliana define a new locus involved in the control of auxin homeostasis."
      Delarue M., Prinsen E., Onckelen H.V., Caboche M., Bellini C.
      Plant J. 14:603-611(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Systematic reverse genetics of transfer-DNA-tagged lines of Arabidopsis. Isolation of mutations in the cytochrome p450 gene superfamily."
      Winkler R.G., Frank M.R., Galbraith D.W., Feyereisen R., Feldmann K.A.
      Plant Physiol. 118:743-750(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    8. "The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450 CYP83B1, a modulator of auxin homeostasis."
      Barlier I., Kowalczyk M., Marchant A., Ljung K., Bhalerao R., Bennett M., Sandberg G., Bellini C.
      Proc. Natl. Acad. Sci. U.S.A. 97:14819-14824(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY AUXIN.
    9. "CYP83b1 is the oxime-metabolizing enzyme in the glucosinolate pathway in Arabidopsis."
      Hansen C.H., Du L., Naur P., Olsen C.E., Axelsen K.B., Hick A.J., Pickett J.A., Halkier B.A.
      J. Biol. Chem. 276:24790-24796(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "CYP83B1, a cytochrome P450 at the metabolic branch point in auxin and indole glucosinolate biosynthesis in Arabidopsis."
      Bak S., Tax F.E., Feldmann K.A., Galbraith D.W., Feyereisen R.
      Plant Cell 13:101-111(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
    11. "The involvement of two p450 enzymes, CYP83B1 and CYP83A1, in auxin homeostasis and glucosinolate biosynthesis."
      Bak S., Feyereisen R.
      Plant Physiol. 127:108-118(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
    12. "Arabidopsis cytochrome P450 cyp83B1 mutations activate the tryptophan biosynthetic pathway."
      Smolen G., Bender J.
      Genetics 160:323-332(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    13. "CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis."
      Naur P., Petersen B.L., Mikkelsen M.D., Bak S., Rasmussen H., Olsen C.E., Halkier B.A.
      Plant Physiol. 133:63-72(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    14. "The photomorphogenesis-related mutant red1 is defective in CYP83B1, a red light-induced gene encoding a cytochrome P450 required for normal auxin homeostasis."
      Hoecker U., Toledo-Ortiz G., Bender J., Quail P.H.
      Planta 219:195-200(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY CONTINUOUS RED LIGHT, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiC83B1_ARATH
    AccessioniPrimary (citable) accession number: O65782
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: August 1, 1998
    Last modified: February 17, 2016
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sur2 mutant phenotype can be complemented by exogenous auxin or low pH medium. Plants overexpresseing SUR2 show decreased apical dominance.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.