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Protein

Cyclic nucleotide-gated ion channel 2

Gene

CNGC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as cyclic nucleotide-gated ion channel. Permeable to potassium and calcium in a cyclic nucleotide-dependent fashion (cAMP or cGMP). Could also transport lithium, cesium and rubium and displays a strong selectivity against sodium. Seems to directly participate in pathogen-induced calcium influx. May function in homeostasis, re-establishing ionic balance after defense action and/or other stimuli. Could mediate the initiation of the developmentally regulated cell death programs.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei600 – 6001cNMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi531 – 661131cNMPAdd
BLAST

GO - Molecular functioni

  • calcium channel activity Source: TAIR
  • calmodulin binding Source: TAIR
  • cAMP binding Source: UniProtKB-KW
  • cGMP binding Source: UniProtKB-KW
  • intracellular cAMP activated cation channel activity Source: TAIR
  • intracellular cyclic nucleotide activated cation channel activity Source: TAIR
  • inward rectifier potassium channel activity Source: TAIR

GO - Biological processi

  • defense response Source: TAIR
  • nitric oxide mediated signal transduction Source: TAIR
  • plant-type hypersensitive response Source: TAIR
  • regulation of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Plant defense, Transport

Keywords - Ligandi

Calmodulin-binding, cAMP, cAMP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2485179. Activation of the phototransduction cascade.
R-ATH-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-ATH-5620916. VxPx cargo-targeting to cilium.

Protein family/group databases

TCDBi1.A.1.5.6. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated ion channel 2
Short name:
AtCNGC2
Alternative name(s):
Cyclic nucleotide- and calmodulin-regulated ion channel 2
Protein DEFENSE NO DEATH 1
Gene namesi
Name:CNGC2
Synonyms:DND1
Ordered Locus Names:At5g15410
ORF Names:T20K14_20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G15410.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 127127CytoplasmicSequence analysisAdd
BLAST
Transmembranei128 – 14821Helical; Name=H1Sequence analysisAdd
BLAST
Topological domaini149 – 16214ExtracellularSequence analysisAdd
BLAST
Transmembranei163 – 18321Helical; Name=H2Sequence analysisAdd
BLAST
Topological domaini184 – 21936CytoplasmicSequence analysisAdd
BLAST
Transmembranei220 – 24021Helical; Name=H3Sequence analysisAdd
BLAST
Topological domaini241 – 25414ExtracellularSequence analysisAdd
BLAST
Transmembranei255 – 27521Helical; Name=H4Sequence analysisAdd
BLAST
Topological domaini276 – 2827CytoplasmicSequence analysis
Transmembranei283 – 30321Helical; Name=H5Sequence analysisAdd
BLAST
Topological domaini304 – 424121ExtracellularSequence analysisAdd
BLAST
Transmembranei425 – 44521Helical; Name=H6Sequence analysisAdd
BLAST
Topological domaini446 – 726281CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726Cyclic nucleotide-gated ion channel 2PRO_0000219330Add
BLAST

Proteomic databases

PaxDbiO65718.

Expressioni

Tissue specificityi

Expressed in the whole plant but only weakly in roots. Strongly expressed in the expanded cotyledons of 14-day-old seedlings and detected later in leaves after the transition to flowering. Also detected in flowers during organ senescence and in the dehiscence zone of siliques.1 Publication

Inductioni

Up-regulated by light. Transiently induced during leaf and culture senescence.1 Publication

Gene expression databases

ExpressionAtlasiO65718. baseline and differential.
GenevisibleiO65718. AT.

Interactioni

Subunit structurei

Homotetramer or heterotetramer (Potential). Binds calmodulin-1/4 with a higher affinity than calmodulin-2/3/5.Curated

GO - Molecular functioni

  • calmodulin binding Source: TAIR

Protein-protein interaction databases

STRINGi3702.AT5G15410.1.

Structurei

3D structure databases

ProteinModelPortaliO65718.
SMRiO65718. Positions 457-646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini666 – 69530IQAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni645 – 66117Calmodulin-bindingBy similarityAdd
BLAST

Domaini

The binding of calmodulin to the C-terminus might interfere with cyclic nucleotide binding and thus channel activation.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 IQ domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000238338.
InParanoidiO65718.
KOiK05391.
OMAiTIHVIEN.
PhylomeDBiO65718.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O65718-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSHPNFIFR WIGLFSDKFR RQTTGIDENS NLQINGGDSS SSGSDETPVL
60 70 80 90 100
SSVECYACTQ VGVPAFHSTS CDQAHAPEWR ASAGSSLVPI QEGSVPNPAR
110 120 130 140 150
TRFRRLKGPF GEVLDPRSKR VQRWNRALLL ARGMALAVDP LFFYALSIGR
160 170 180 190 200
TTGPACLYMD GAFAAVVTVL RTCLDAVHLW HVWLQFRLAY VSRESLVVGC
210 220 230 240 250
GKLVWDPRAI ASHYARSLTG FWFDVIVILP VPQAVFWLVV PKLIREEKVK
260 270 280 290 300
LIMTILLLIF LFQFLPKIYH CICLMRRMQK VTGYIFGTIW WGFALNLIAY
310 320 330 340 350
FIASHVAGGC WYVLAIQRVA SCIRQQCMRT GNCNLSLACK EEVCYQFVSP
360 370 380 390 400
TSTVGYPCLS GNLTSVVNKP MCLDSNGPFR YGIYRWALPV ISSNSLAVKI
410 420 430 440 450
LYPIFWGLMT LSTFANDLEP TSNWLEVIFS IVMVLSGLLL FTLLIGNIQV
460 470 480 490 500
FLHAVMAKKR KMQIRCRDME WWMKRRQLPS RLRQRVRRFE RQRWNALGGE
510 520 530 540 550
DELELIHDLP PGLRRDIKRY LCFDLINKVP LFRGMDDLIL DNICDRAKPR
560 570 580 590 600
VFSKDEKIIR EGDPVQRMIF IMRGRVKRIQ SLSKGVLATS TLEPGGYLGD
610 620 630 640 650
ELLSWCLRRP FLDRLPPSSA TFVCLENIEA FSLGSEDLRY ITDHFRYKFA
660 670 680 690 700
NERLKRTARY YSSNWRTWAA VNIQMAWRRR RKRTRGENIG GSMSPVSENS
710 720
IEGNSERRLL QYAAMFMSIR PHDHLE
Length:726
Mass (Da):83,241
Last modified:August 1, 1998 - v1
Checksum:i450097F2C392D8D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16328 Genomic DNA. Translation: CAA76179.1.
AF067798 mRNA. Translation: AAC78613.1.
AF280939 Genomic DNA. Translation: AAF86351.1.
AL391143 Genomic DNA. Translation: CAC01740.1.
CP002688 Genomic DNA. Translation: AED92158.1.
PIRiT51519.
RefSeqiNP_197045.1. NM_121545.4. [O65718-1]
UniGeneiAt.20813.
At.74751.

Genome annotation databases

EnsemblPlantsiAT5G15410.1; AT5G15410.1; AT5G15410. [O65718-1]
GeneIDi831393.
KEGGiath:AT5G15410.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16328 Genomic DNA. Translation: CAA76179.1.
AF067798 mRNA. Translation: AAC78613.1.
AF280939 Genomic DNA. Translation: AAF86351.1.
AL391143 Genomic DNA. Translation: CAC01740.1.
CP002688 Genomic DNA. Translation: AED92158.1.
PIRiT51519.
RefSeqiNP_197045.1. NM_121545.4. [O65718-1]
UniGeneiAt.20813.
At.74751.

3D structure databases

ProteinModelPortaliO65718.
SMRiO65718. Positions 457-646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G15410.1.

Protein family/group databases

TCDBi1.A.1.5.6. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiO65718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G15410.1; AT5G15410.1; AT5G15410. [O65718-1]
GeneIDi831393.
KEGGiath:AT5G15410.

Organism-specific databases

TAIRiAT5G15410.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000238338.
InParanoidiO65718.
KOiK05391.
OMAiTIHVIEN.
PhylomeDBiO65718.

Enzyme and pathway databases

ReactomeiR-ATH-2485179. Activation of the phototransduction cascade.
R-ATH-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-ATH-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

PROiO65718.

Gene expression databases

ExpressionAtlasiO65718. baseline and differential.
GenevisibleiO65718. AT.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and partial characterization of two putative cyclic nucleotide-regulated ion channels from Arabidopsis thaliana, designated CNGC1 and CNGC2."
    Koehler C., Neuhaus G.
    Plant Gene Register PGR98-062
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: cv. Columbia.
  2. "Cloning and first functional characterization of a plant cyclic nucleotide-gated cation channel."
    Leng Q., Mercier R.W., Yao W., Berkowitz G.A.
    Plant Physiol. 121:753-761(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: cv. Columbia.
  3. "The Arabidopsis dnd1 'defense, no death' gene encodes a mutated cyclic nucleotide-gated ion channel."
    Clough S.J., Fengler K.A., Yu I.-C., Lippok B., Smith R.K. Jr., Bent A.F.
    Proc. Natl. Acad. Sci. U.S.A. 97:9323-9328(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANT DND1-1.
    Strain: cv. Columbia.
  4. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana."
    Koehler C., Merkle T., Neuhaus G.
    Plant J. 18:97-104(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALMODULIN.
  7. "Characterisation of calmodulin binding to cyclic nucleotide-gated ion channels from Arabidopsis thaliana."
    Koehler C., Neuhaus G.
    FEBS Lett. 471:133-136(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CALMODULIN-BINDING DOMAIN.
  8. "Developmentally regulated expression of a cyclic nucleotide-gated ion channel from Arabidopsis indicates its involvement in programmed cell death."
    Koehler C., Merkle T., Roby D., Neuhaus G.
    Planta 213:327-332(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  9. Cited for: GENE FAMILY, NOMENCLATURE.
  10. "A cyclic nucleotide-gated ion channel, CNGC2, is crucial for plant development and adaptation to calcium stress."
    Chan C.W.M., Schorrak L.M., Smith R.K. Jr., Bent A.F., Sussman M.R.
    Plant Physiol. 132:728-731(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NULL MUTANTS.

Entry informationi

Entry nameiCNGC2_ARATH
AccessioniPrimary (citable) accession number: O65718
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Loss-of-function mutations cngc2-1 (dnd1-1) or cncg2-2 results in the loss of the hypersensitive response and leads to a broad spectrum disease resistance. These mutations lead to a specific and dramatic calcium hypersensitivity that results in severe reductions in plant size and seed yield.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.