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O65595

- KPYC_ARATH

UniProt

O65595 - KPYC_ARATH

Protein

Probable pyruvate kinase, cytosolic isozyme

Gene

At4g26390

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371SubstrateBy similarity
    Metal bindingi39 – 391PotassiumBy similarity
    Metal bindingi41 – 411PotassiumBy similarity
    Metal bindingi71 – 711PotassiumBy similarity
    Metal bindingi72 – 721Potassium; via carbonyl oxygenBy similarity
    Sitei227 – 2271Transition state stabilizerBy similarity
    Metal bindingi229 – 2291MagnesiumBy similarity
    Binding sitei252 – 2521Substrate; via amide nitrogenBy similarity
    Metal bindingi253 – 2531MagnesiumBy similarity
    Binding sitei253 – 2531Substrate; via amide nitrogenBy similarity
    Binding sitei285 – 2851SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciARA:AT4G26390-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Ordered Locus Names:At4g26390
    ORF Names:M3E9.180
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G26390.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 497497Probable pyruvate kinase, cytosolic isozymePRO_0000112123Add
    BLAST

    Proteomic databases

    PaxDbiO65595.
    PRIDEiO65595.

    Expressioni

    Gene expression databases

    GenevestigatoriO65595.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT4G26390.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO65595.
    SMRiO65595. Positions 8-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    InParanoidiO65595.
    KOiK00873.
    OMAiCRCENTS.
    PhylomeDBiO65595.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O65595-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMEQRPKTK IVCTLGPASR SVPMVEKLLM AGMSVARFNF SHGSYEYHQE    50
    TLDNLRQAML NTGMLCAVML DTKGPEIRTG FLKDGKPIQL KQGQEITIST 100
    DYDLKGDEKT ICMSYKKLAQ DVNPGMVILC ADGTISLKVL SCDKEKGTVR 150
    CRCENTSMLG ERKNVNLPGV VVDLPTLTEK DKQDILEWGV PNQIDMIALS 200
    FVRKGSDLVQ VRKLLGKHAK TILLMSKVEN QEGVANFDDI LINSDAFMIA 250
    RGDLGMEIPI EKIFLAQKVM IYKCNFMGKP VVTATQMLES MIKSPRPTRA 300
    EATDVANAVL DGTDCVMLSG ETAAGAYPEL AVRTMAKICV EAESTLDYGD 350
    IFKRIMLHAA VPMSPMESLA SSAVRTATSS RATLMMVLTR GGSTARLVAK 400
    YRPGIPILSV VVPEITSDSF DWACSNEAPA RHSLIYRGLV PVLYAGSARA 450
    SIDESTEETL EFASEYGKKK QLCKTGDSVV ALFRTGNAIV IKILTVK 497
    Length:497
    Mass (Da):54,319
    Last modified:August 1, 1998 - v1
    Checksum:i45D43EF54A89FE62
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL022223 Genomic DNA. Translation: CAA18231.1.
    AL161565 Genomic DNA. Translation: CAB79494.1.
    CP002687 Genomic DNA. Translation: AEE85192.1.
    PIRiT05065.
    RefSeqiNP_194369.1. NM_118772.1.
    UniGeneiAt.54520.

    Genome annotation databases

    EnsemblPlantsiAT4G26390.1; AT4G26390.1; AT4G26390.
    GeneIDi828745.
    KEGGiath:AT4G26390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL022223 Genomic DNA. Translation: CAA18231.1 .
    AL161565 Genomic DNA. Translation: CAB79494.1 .
    CP002687 Genomic DNA. Translation: AEE85192.1 .
    PIRi T05065.
    RefSeqi NP_194369.1. NM_118772.1.
    UniGenei At.54520.

    3D structure databases

    ProteinModelPortali O65595.
    SMRi O65595. Positions 8-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G26390.1-P.

    Proteomic databases

    PaxDbi O65595.
    PRIDEi O65595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G26390.1 ; AT4G26390.1 ; AT4G26390 .
    GeneIDi 828745.
    KEGGi ath:AT4G26390.

    Organism-specific databases

    TAIRi AT4G26390.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    InParanoidi O65595.
    KOi K00873.
    OMAi CRCENTS.
    PhylomeDBi O65595.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci ARA:AT4G26390-MONOMER.

    Gene expression databases

    Genevestigatori O65595.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiKPYC_ARATH
    AccessioniPrimary (citable) accession number: O65595
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3