Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fructose-bisphosphate aldolase 5, cytosolic

Gene

FBA5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Fructose-bisphosphate aldolase that plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Active sitei183Proton acceptorBy similarity1
Active sitei225Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Binding sitei298SubstrateBy similarity1
Sitei358Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: UniProtKB

GO - Biological processi

  • gluconeogenesis Source: UniProtKB
  • glycolytic process Source: UniProtKB

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

BioCyciARA:AT4G26530-MONOMER
ReactomeiR-ATH-114608 Platelet degranulation
R-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
R-ATH-70350 Fructose catabolism
UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 5, cytosolicCurated (EC:4.1.2.13By similarity)
Short name:
AtFBA51 Publication
Gene namesi
Name:FBA51 Publication
Ordered Locus Names:At4g26530Imported
ORF Names:M3E9.40Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26530
TAIRilocus:2131513 AT4G26530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004372392 – 358Fructose-bisphosphate aldolase 5, cytosolicAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei68S-glutathionyl cysteine; transientBy similarity1
Modified residuei173S-glutathionyl cysteine; transient; alternateBy similarity1
Modified residuei173S-nitrosocysteine; transient; alternateBy similarity1
Modified residuei350PhosphoserineBy similarity1

Post-translational modificationi

S-glutathionylated at Cys-68 and Cys-173.By similarity
S-nitrosylated at Cys-173.By similarity

Keywords - PTMi

Acetylation, Glutathionylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiO65581
PRIDEiO65581
ProMEXiO65581

PTM databases

iPTMnetiO65581

Expressioni

Tissue specificityi

Expressed in rosette leaves and cauline leaves.1 Publication

Inductioni

Down-regulated by cadmium (PubMed:16797112). Induced by sucrose (PubMed:22561114). Induced by abiotic stresses (PubMed:22561114).2 Publications

Gene expression databases

ExpressionAtlasiO65581 baseline and differential

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with TRX3 (PubMed:15352244).By similarity1 Publication

Protein-protein interaction databases

IntActiO65581, 2 interactors
STRINGi3702.AT4G26530.1

Structurei

3D structure databases

ProteinModelPortaliO65581
SMRiO65581
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 268Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
HOGENOMiHOG000220876
KOiK01623
OMAiMTHQYPA
OrthoDBiEOG09360ENE
PhylomeDBiO65581

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFVGKYAD ELIKTAKYIA TPGKGILAAD ESTGTIGKRF ASINVENIES
60 70 80 90 100
NRQALRELLF TSPGTFPCLS GVILFEETLY QKTTDGKPFV ELLMENGVIP
110 120 130 140 150
GIKVDKGVVD LAGTNGETTT QGLDSLGARC QEYYKAGARF AKWRAVLKIG
160 170 180 190 200
ATEPSELSIQ ENAKGLARYA IICQENGLVP IVEPEVLTDG SHDIKKCAAV
210 220 230 240 250
TETVLAAVYK ALNDHHVLLE GTLLKPNMVT PGSDSPKVAP EVIAEYTVTA
260 270 280 290 300
LRRTVPPAVP GIVFLSGGQS EEEATLNLNA MNKLDVLKPW TLTFSFGRAL
310 320 330 340 350
QQSTLKAWAG KTENVAKAQA TFLTRCKGNS DATLGKYTGG ASGDSAASES

LYEEGYKY
Length:358
Mass (Da):38,294
Last modified:August 1, 1998 - v1
Checksum:i351B4420956E64C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022223 Genomic DNA Translation: CAA18217.1
AL161565 Genomic DNA Translation: CAB79508.1
CP002687 Genomic DNA Translation: AEE85214.1
CP002687 Genomic DNA Translation: AEE85215.1
CP002687 Genomic DNA Translation: ANM66838.1
BT002006 mRNA Translation: AAN72017.1
BT008844 mRNA Translation: AAP68283.1
AY087376 mRNA Translation: AAM64926.1
PIRiT05051
RefSeqiNP_001031721.1, NM_001036644.2
NP_001328708.1, NM_001341809.1
NP_194383.1, NM_118786.3
UniGeneiAt.57980

Genome annotation databases

EnsemblPlantsiAT4G26530.1; AT4G26530.1; AT4G26530
AT4G26530.2; AT4G26530.2; AT4G26530
AT4G26530.3; AT4G26530.3; AT4G26530
GeneIDi828759
GrameneiAT4G26530.1; AT4G26530.1; AT4G26530
AT4G26530.2; AT4G26530.2; AT4G26530
AT4G26530.3; AT4G26530.3; AT4G26530
KEGGiath:AT4G26530

Similar proteinsi

Entry informationi

Entry nameiALFC5_ARATH
AccessioniPrimary (citable) accession number: O65581
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health