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Protein

Villin-4

Gene

VLN4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds actin and actin filament bundles in a Ca2+-insensitive manner, but caps the barbed end of actin filaments and is able to sever them in a calcium-dependent manner. Involved in root hair growth through regulating actin organization in a Ca2+-dependent manner.2 Publications

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: InterPro
  • actin filament capping Source: UniProtKB-KW
  • actin filament depolymerization Source: TAIR
  • actin filament organization Source: TAIR
  • actin filament severing Source: TAIR
  • cytoplasmic streaming Source: TAIR
  • response to abscisic acid Source: TAIR
  • root hair elongation Source: TAIR

Keywordsi

Molecular functionActin capping, Actin-binding
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Villin-41 Publication
Gene namesi
Name:VLN41 Publication
Ordered Locus Names:At4g30160Imported
ORF Names:F6G3.190Imported, F9N11.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G30160.
TAIRilocus:2128911. AT4G30160.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

No changes in the primary root length, but shorter root hairs. Increased sensitivity to latrunculin B (LatB) and instability of actin filaments.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187351 – 974Villin-4Add BLAST974

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei777PhosphoserineCombined sources1
Modified residuei787PhosphoserineCombined sources1
Modified residuei890PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO65570.
PRIDEiO65570.

PTM databases

iPTMnetiO65570.

Expressioni

Tissue specificityi

Preferentially expressed in vegetative tissues. Detected in the whole seedling, hypocotyl, cotyledon, primary root, roots hair cells and trichomes. Expressed in flowers but not in the silique.2 Publications

Gene expression databases

ExpressionAtlasiO65570. baseline and differential.
GenevisibleiO65570. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi14426. 1 interactor.
STRINGi3702.AT4G30160.2.

Structurei

Secondary structure

1974
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni917 – 919Combined sources3
Helixi933 – 939Combined sources7
Helixi942 – 949Combined sources8
Helixi953 – 958Combined sources6
Helixi961 – 970Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VNTNMR-A912-974[»]
ProteinModelPortaliO65570.
SMRiO65570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati29 – 79Gelsolin-like 1Sequence analysisAdd BLAST51
Repeati150 – 190Gelsolin-like 2Sequence analysisAdd BLAST41
Repeati262 – 305Gelsolin-like 3Sequence analysisAdd BLAST44
Repeati394 – 451Gelsolin-like 4Sequence analysisAdd BLAST58
Repeati532 – 572Gelsolin-like 5Sequence analysisAdd BLAST41
Repeati634 – 675Gelsolin-like 6Sequence analysisAdd BLAST42
Domaini909 – 974HPPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO65570.
OMAiWIGAEAN.
OrthoDBiEOG0936017U.
PhylomeDBiO65570.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiView protein in InterPro
IPR029006. ADF-H/Gelsolin-like_dom_sf.
IPR007123. Gelsolin-like_dom.
IPR030009. Villin-2/3/4/5_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
IPR036886. Villin_headpiece_dom_sf.
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF51. PTHR11977:SF51. 1 hit.
PfamiView protein in Pfam
PF00626. Gelsolin. 4 hits.
PF02209. VHP. 1 hit.
PRINTSiPR00597. GELSOLIN.
SMARTiView protein in SMART
SM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiView protein in PROSITE
PS51089. HP. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O65570-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSMRDLDP AFQGAGQKAG IEIWRIENFI PTPIPKSSIG KFFTGDSYIV
60 70 80 90 100
LKTTALKTGA LRHDIHYWLG KDTSQDEAGT AAVKTVELDA ALGGRAVQYR
110 120 130 140 150
EVQGHETEKF LSYFKPCIIP QEGGVASGFK HVVAEEHITR LFVCRGKHVV
160 170 180 190 200
HVKEVPFARS SLNHDDIYIL DTKSKIFQFN GSNSSIQERA KALEVVQYIK
210 220 230 240 250
DTYHDGTCEV ATVEDGKLMA DADSGEFWGF FGGFAPLPRK TANDEDKTYN
260 270 280 290 300
SDITRLFCVE KGQANPVEGD TLKREMLDTN KCYILDCGIE VFVWMGRTTS
310 320 330 340 350
LDDRKIASKA AEEMIRSSER PKSQMIRIIE GFETVPFRSK FESWTQETNT
360 370 380 390 400
TVSEDGRGRV AALLQRQGVN VRGLMKAAPP KEEPQVFIDC TGNLQVWRVN
410 420 430 440 450
GQAKTLLQAA DHSKFYSGDC YVFQYSYPGE EKEEVLIGTW FGKQSVEEER
460 470 480 490 500
GSAVSMASKM VESMKFVPAQ ARIYEGKEPI QFFVIMQSFI VFKGGISSGY
510 520 530 540 550
KKYIAEKEVD DDTYNENGVA LFRIQGSGPE NMQAIQVDPV AASLNSSYYY
560 570 580 590 600
ILHNDSSVFT WAGNLSTATD QELAERQLDL IKPNQQSRAQ KEGSESEQFW
610 620 630 640 650
ELLGGKAEYS SQKLTKEPER DPHLFSCTFT KEVLKVTEIY NFTQDDLMTE
660 670 680 690 700
DIFIIDCHSE IFVWVGQEVV PKNKLLALTI GEKFIEKDSL LEKLSPEAPI
710 720 730 740 750
YVIMEGGEPS FFTRFFTSWD SSKSAMHGNS FQRKLKIVKN GGTPVADKPK
760 770 780 790 800
RRTPASYGGR ASVPDKSQQR SRSMSFSPDR VRVRGRSPAF NALAATFESQ
810 820 830 840 850
NARNLSTPPP VVRKLYPRSV TPDSSKFAPA PKSSAIASRS ALFEKIPPQE
860 870 880 890 900
PSIPKPVKAS PKTPESPAPE SNSKEQEEKK ENDKEEGSMS SRIESLTIQE
910 920 930 940 950
DAKEGVEDEE DLPAHPYDRL KTTSTDPVSD IDVTRREAYL SSEEFKEKFG
960 970
MTKEAFYKLP KWKQNKFKMA VQLF
Length:974
Mass (Da):109,327
Last modified:August 1, 1998 - v1
Checksum:iE81BCBA2191ECB7D
GO
Isoform 2 (identifier: O65570-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     635-635: K → KVRILLKSFF

Note: No experimental confirmation available. Derived from EST data.Curated
Show »
Length:983
Mass (Da):110,432
Checksum:iC4D5C4C059D5CCE2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056809635K → KVRILLKSFF in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12782 mRNA. Translation: CAA73320.1.
AL078464 Genomic DNA. Translation: CAB43851.1.
AL109796 Genomic DNA. Translation: CAB52460.1.
AL161576 Genomic DNA. Translation: CAB81009.1.
CP002687 Genomic DNA. Translation: AEE85727.1.
CP002687 Genomic DNA. Translation: AEE85728.1.
CP002687 Genomic DNA. Translation: ANM68140.1.
CP002687 Genomic DNA. Translation: ANM68141.1.
AK117296 mRNA. Translation: BAC41968.1.
BT005980 mRNA. Translation: AAO64915.1.
PIRiT14076.
RefSeqiNP_001190869.1. NM_001203940.1. [O65570-2]
NP_001329917.1. NM_001342000.1. [O65570-1]
NP_001329918.1. NM_001341999.1. [O65570-1]
NP_194745.1. NM_119162.5. [O65570-1]
UniGeneiAt.64.
At.67575.

Genome annotation databases

EnsemblPlantsiAT4G30160.1; AT4G30160.1; AT4G30160. [O65570-1]
AT4G30160.2; AT4G30160.2; AT4G30160. [O65570-2]
AT4G30160.3; AT4G30160.3; AT4G30160. [O65570-1]
AT4G30160.4; AT4G30160.4; AT4G30160. [O65570-1]
GeneIDi829139.
GrameneiAT4G30160.1; AT4G30160.1; AT4G30160.
AT4G30160.2; AT4G30160.2; AT4G30160.
AT4G30160.3; AT4G30160.3; AT4G30160.
AT4G30160.4; AT4G30160.4; AT4G30160.
KEGGiath:AT4G30160.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVILI4_ARATH
AccessioniPrimary (citable) accession number: O65570
Secondary accession number(s): F4JPJ2, Q541Y5, Q9SZW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: August 1, 1998
Last modified: November 22, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families