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Protein

Villin-4

Gene

VLN4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds actin and actin filament bundles in a Ca2+-insensitive manner, but caps the barbed end of actin filaments and is able to sever them in a calcium-dependent manner. Involved in root hair growth through regulating actin organization in a Ca2+-dependent manner.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Actin capping

Keywords - Ligandi

Actin-binding, Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Villin-41 Publication
Gene namesi
Name:VLN41 Publication
Ordered Locus Names:At4g30160Imported
ORF Names:F6G3.190Imported, F9N11.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30160.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

No changes in the primary root length, but shorter root hairs. Increased sensitivity to latrunculin B (LatB) and instability of actin filaments.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 974974Villin-4PRO_0000218735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei777 – 7771PhosphoserineCombined sources
Modified residuei787 – 7871PhosphoserineCombined sources
Modified residuei890 – 8901PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO65570.
PRIDEiO65570.

PTM databases

iPTMnetiO65570.

Expressioni

Tissue specificityi

Preferentially expressed in vegetative tissues. Detected in the whole seedling, hypocotyl, cotyledon, primary root, roots hair cells and trichomes. Expressed in flowers but not in the silique.2 Publications

Gene expression databases

ExpressionAtlasiO65570. baseline and differential.
GenevisibleiO65570. AT.

Interactioni

Protein-protein interaction databases

BioGridi14426. 1 interaction.
STRINGi3702.AT4G30160.2.

Structurei

3D structure databases

ProteinModelPortaliO65570.
SMRiO65570. Positions 10-723, 915-974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 7951Gelsolin-like 1Sequence analysisAdd
BLAST
Repeati150 – 19041Gelsolin-like 2Sequence analysisAdd
BLAST
Repeati262 – 30544Gelsolin-like 3Sequence analysisAdd
BLAST
Repeati394 – 45158Gelsolin-like 4Sequence analysisAdd
BLAST
Repeati532 – 57241Gelsolin-like 5Sequence analysisAdd
BLAST
Repeati634 – 67542Gelsolin-like 6Sequence analysisAdd
BLAST
Domaini909 – 97466HPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated
Contains 6 gelsolin-like repeats.Curated
Contains 1 HP (headpiece) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO65570.
OMAiFMGDSYV.
OrthoDBiEOG0936017U.
PhylomeDBiO65570.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR030009. Villin-2/3/4/5_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF47. PTHR11977:SF47. 2 hits.
PfamiPF00626. Gelsolin. 4 hits.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O65570-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSMRDLDP AFQGAGQKAG IEIWRIENFI PTPIPKSSIG KFFTGDSYIV
60 70 80 90 100
LKTTALKTGA LRHDIHYWLG KDTSQDEAGT AAVKTVELDA ALGGRAVQYR
110 120 130 140 150
EVQGHETEKF LSYFKPCIIP QEGGVASGFK HVVAEEHITR LFVCRGKHVV
160 170 180 190 200
HVKEVPFARS SLNHDDIYIL DTKSKIFQFN GSNSSIQERA KALEVVQYIK
210 220 230 240 250
DTYHDGTCEV ATVEDGKLMA DADSGEFWGF FGGFAPLPRK TANDEDKTYN
260 270 280 290 300
SDITRLFCVE KGQANPVEGD TLKREMLDTN KCYILDCGIE VFVWMGRTTS
310 320 330 340 350
LDDRKIASKA AEEMIRSSER PKSQMIRIIE GFETVPFRSK FESWTQETNT
360 370 380 390 400
TVSEDGRGRV AALLQRQGVN VRGLMKAAPP KEEPQVFIDC TGNLQVWRVN
410 420 430 440 450
GQAKTLLQAA DHSKFYSGDC YVFQYSYPGE EKEEVLIGTW FGKQSVEEER
460 470 480 490 500
GSAVSMASKM VESMKFVPAQ ARIYEGKEPI QFFVIMQSFI VFKGGISSGY
510 520 530 540 550
KKYIAEKEVD DDTYNENGVA LFRIQGSGPE NMQAIQVDPV AASLNSSYYY
560 570 580 590 600
ILHNDSSVFT WAGNLSTATD QELAERQLDL IKPNQQSRAQ KEGSESEQFW
610 620 630 640 650
ELLGGKAEYS SQKLTKEPER DPHLFSCTFT KEVLKVTEIY NFTQDDLMTE
660 670 680 690 700
DIFIIDCHSE IFVWVGQEVV PKNKLLALTI GEKFIEKDSL LEKLSPEAPI
710 720 730 740 750
YVIMEGGEPS FFTRFFTSWD SSKSAMHGNS FQRKLKIVKN GGTPVADKPK
760 770 780 790 800
RRTPASYGGR ASVPDKSQQR SRSMSFSPDR VRVRGRSPAF NALAATFESQ
810 820 830 840 850
NARNLSTPPP VVRKLYPRSV TPDSSKFAPA PKSSAIASRS ALFEKIPPQE
860 870 880 890 900
PSIPKPVKAS PKTPESPAPE SNSKEQEEKK ENDKEEGSMS SRIESLTIQE
910 920 930 940 950
DAKEGVEDEE DLPAHPYDRL KTTSTDPVSD IDVTRREAYL SSEEFKEKFG
960 970
MTKEAFYKLP KWKQNKFKMA VQLF
Length:974
Mass (Da):109,327
Last modified:August 1, 1998 - v1
Checksum:iE81BCBA2191ECB7D
GO
Isoform 2 (identifier: O65570-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     635-635: K → KVRILLKSFF

Note: No experimental confirmation available. Derived from EST data.Curated
Show »
Length:983
Mass (Da):110,432
Checksum:iC4D5C4C059D5CCE2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei635 – 6351K → KVRILLKSFF in isoform 2. CuratedVSP_056809

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12782 mRNA. Translation: CAA73320.1.
AL078464 Genomic DNA. Translation: CAB43851.1.
AL109796 Genomic DNA. Translation: CAB52460.1.
AL161576 Genomic DNA. Translation: CAB81009.1.
CP002687 Genomic DNA. Translation: AEE85727.1.
CP002687 Genomic DNA. Translation: AEE85728.1.
AK117296 mRNA. Translation: BAC41968.1.
BT005980 mRNA. Translation: AAO64915.1.
PIRiT14076.
RefSeqiNP_001190869.1. NM_001203940.1. [O65570-2]
NP_194745.1. NM_119162.4. [O65570-1]
UniGeneiAt.64.
At.67575.

Genome annotation databases

EnsemblPlantsiAT4G30160.1; AT4G30160.1; AT4G30160. [O65570-1]
AT4G30160.2; AT4G30160.2; AT4G30160. [O65570-2]
GeneIDi829139.
KEGGiath:AT4G30160.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12782 mRNA. Translation: CAA73320.1.
AL078464 Genomic DNA. Translation: CAB43851.1.
AL109796 Genomic DNA. Translation: CAB52460.1.
AL161576 Genomic DNA. Translation: CAB81009.1.
CP002687 Genomic DNA. Translation: AEE85727.1.
CP002687 Genomic DNA. Translation: AEE85728.1.
AK117296 mRNA. Translation: BAC41968.1.
BT005980 mRNA. Translation: AAO64915.1.
PIRiT14076.
RefSeqiNP_001190869.1. NM_001203940.1. [O65570-2]
NP_194745.1. NM_119162.4. [O65570-1]
UniGeneiAt.64.
At.67575.

3D structure databases

ProteinModelPortaliO65570.
SMRiO65570. Positions 10-723, 915-974.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14426. 1 interaction.
STRINGi3702.AT4G30160.2.

PTM databases

iPTMnetiO65570.

Proteomic databases

PaxDbiO65570.
PRIDEiO65570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30160.1; AT4G30160.1; AT4G30160. [O65570-1]
AT4G30160.2; AT4G30160.2; AT4G30160. [O65570-2]
GeneIDi829139.
KEGGiath:AT4G30160.

Organism-specific databases

TAIRiAT4G30160.

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO65570.
OMAiFMGDSYV.
OrthoDBiEOG0936017U.
PhylomeDBiO65570.

Miscellaneous databases

PROiO65570.

Gene expression databases

ExpressionAtlasiO65570. baseline and differential.
GenevisibleiO65570. AT.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR030009. Villin-2/3/4/5_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF47. PTHR11977:SF47. 2 hits.
PfamiPF00626. Gelsolin. 4 hits.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVILI4_ARATH
AccessioniPrimary (citable) accession number: O65570
Secondary accession number(s): F4JPJ2, Q541Y5, Q9SZW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.