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Protein

CBL-interacting serine/threonine-protein kinase 6

Gene

CIPK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Downstream of CBL1, CBL2, CBL3 and CBL9, regulates by phosphorylation the K+ conductance and uptake of AKT1. Binds to CBL4 to modulate AKT2 activity by promoting a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53ATPPROSITE-ProRule annotation1
Active sitei146Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • basipetal auxin transport Source: TAIR
  • hyperosmotic salinity response Source: TAIR
  • intracellular signal transduction Source: GO_Central
  • multicellular organism development Source: TAIR
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 6 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.14
SOS2-like protein kinase PKS4
SOS3-interacting protein 3
Gene namesi
Name:CIPK6
Synonyms:PKS4, SIP3, SnRK3.14
Ordered Locus Names:At4g30960
ORF Names:F6I18.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G30960
TAIRilocus:2126749 AT4G30960

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Delayed development and flowering.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi182T → D: Increased activity and autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003372091 – 441CBL-interacting serine/threonine-protein kinase 6Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei168PhosphoserineBy similarity1
Modified residuei182PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO65554

PTM databases

iPTMnetiO65554

Expressioni

Tissue specificityi

Expressed in roots and shoots.1 Publication

Gene expression databases

ExpressionAtlasiO65554 baseline and differential
GenevisibleiO65554 AT

Interactioni

Subunit structurei

Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1, AKT2,CBL1, CBL2, CBL3, CBL4/SOS3 and CBL9.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi14508, 11 interactors
DIPiDIP-34749N
IntActiO65554, 12 interactors
STRINGi3702.AT4G30960.1

Structurei

3D structure databases

ProteinModelPortaliO65554
SMRiO65554
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 278Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini310 – 334NAFPROSITE-ProRule annotationAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 193Activation loopBy similarityAdd BLAST30
Regioni341 – 371PPIBy similarityAdd BLAST31

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
HOGENOMiHOG000233016
InParanoidiO65554
OMAiKVEMRFA
OrthoDBiEOG09360BAB
PhylomeDBiO65554

Family and domain databases

InterProiView protein in InterPro
IPR020636 Ca/CaM-dep_Ca-dep_prot_Kinase
IPR011009 Kinase-like_dom_sf
IPR018451 NAF/FISL_domain
IPR004041 NAF_dom
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR43895 PTHR43895, 1 hit
PfamiView protein in Pfam
PF03822 NAF, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50816 NAF, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O65554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGAKPVENG SDGGSSTGLL HGRYELGRLL GHGTFAKVYH ARNIQTGKSV
60 70 80 90 100
AMKVVGKEKV VKVGMVDQIK REISVMRMVK HPNIVELHEV MASKSKIYFA
110 120 130 140 150
MELVRGGELF AKVAKGRLRE DVARVYFQQL ISAVDFCHSR GVYHRDLKPE
160 170 180 190 200
NLLLDEEGNL KVTDFGLSAF TEHLKQDGLL HTTCGTPAYV APEVILKKGY
210 220 230 240 250
DGAKADLWSC GVILFVLLAG YLPFQDDNLV NMYRKIYRGD FKCPGWLSSD
260 270 280 290 300
ARRLVTKLLD PNPNTRITIE KVMDSPWFKK QATRSRNEPV AATITTTEED
310 320 330 340 350
VDFLVHKSKE ETETLNAFHI IALSEGFDLS PLFEEKKKEE KREMRFATSR
360 370 380 390 400
PASSVISSLE EAARVGNKFD VRKSESRVRI EGKQNGRKGK LAVEAEIFAV
410 420 430 440
APSFVVVEVK KDHGDTLEYN NFCSTALRPA LKDIFWTSTP A
Length:441
Mass (Da):49,357
Last modified:August 1, 1998 - v1
Checksum:i469728C6CB83F428
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285106 mRNA Translation: AAF86505.1
AF339145 mRNA Translation: AAK26843.1
AL022198 Genomic DNA Translation: CAA18197.1
AL161578 Genomic DNA Translation: CAB79814.1
CP002687 Genomic DNA Translation: AEE85835.1
AF436831 mRNA Translation: AAL32013.1
AY035046 mRNA Translation: AAK59551.1
AY051051 mRNA Translation: AAK93728.1
PIRiE85362
RefSeqiNP_194825.1, NM_119244.3
UniGeneiAt.2154

Genome annotation databases

EnsemblPlantsiAT4G30960.1; AT4G30960.1; AT4G30960
GeneIDi829221
GrameneiAT4G30960.1; AT4G30960.1; AT4G30960
KEGGiath:AT4G30960

Similar proteinsi

Entry informationi

Entry nameiCIPK6_ARATH
AccessioniPrimary (citable) accession number: O65554
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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