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Protein

CBL-interacting serine/threonine-protein kinase 6

Gene

CIPK6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Downstream of CBL1, CBL2, CBL3 and CBL9, regulates by phosphorylation the K+ conductance and uptake of AKT1. Binds to CBL4 to modulate AKT2 activity by promoting a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531ATPPROSITE-ProRule annotation
Active sitei146 – 1461Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • basipetal auxin transport Source: TAIR
  • hyperosmotic salinity response Source: TAIR
  • multicellular organism development Source: TAIR
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G30960-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 6 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.14
SOS2-like protein kinase PKS4
SOS3-interacting protein 3
Gene namesi
Name:CIPK6
Synonyms:PKS4, SIP3, SnRK3.14
Ordered Locus Names:At4g30960
ORF Names:F6I18.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30960.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Delayed development and flowering.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi182 – 1821T → D: Increased activity and autophosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441CBL-interacting serine/threonine-protein kinase 6PRO_0000337209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei168 – 1681PhosphoserineBy similarity
Modified residuei182 – 1821PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO65554.
PRIDEiO65554.

PTM databases

iPTMnetiO65554.

Expressioni

Tissue specificityi

Expressed in roots and shoots.1 Publication

Gene expression databases

GenevisibleiO65554. AT.

Interactioni

Subunit structurei

Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1, AKT2,CBL1, CBL2, CBL3, CBL4/SOS3 and CBL9.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAM4P258542EBI-537615,EBI-1235664
CBL1O814453EBI-537615,EBI-974530
CBL2Q8LAS73EBI-537615,EBI-485991
CBL3Q8LEM74EBI-537615,EBI-637358

Protein-protein interaction databases

BioGridi14508. 11 interactions.
DIPiDIP-34749N.
IntActiO65554. 12 interactions.
STRINGi3702.AT4G30960.1.

Structurei

3D structure databases

ProteinModelPortaliO65554.
SMRiO65554. Positions 22-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 278255Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini310 – 33425NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 19330Activation loopBy similarityAdd
BLAST
Regioni341 – 37131PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO65554.
OMAiISKVMDS.
OrthoDBiEOG09360BAB.
PhylomeDBiO65554.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O65554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGAKPVENG SDGGSSTGLL HGRYELGRLL GHGTFAKVYH ARNIQTGKSV
60 70 80 90 100
AMKVVGKEKV VKVGMVDQIK REISVMRMVK HPNIVELHEV MASKSKIYFA
110 120 130 140 150
MELVRGGELF AKVAKGRLRE DVARVYFQQL ISAVDFCHSR GVYHRDLKPE
160 170 180 190 200
NLLLDEEGNL KVTDFGLSAF TEHLKQDGLL HTTCGTPAYV APEVILKKGY
210 220 230 240 250
DGAKADLWSC GVILFVLLAG YLPFQDDNLV NMYRKIYRGD FKCPGWLSSD
260 270 280 290 300
ARRLVTKLLD PNPNTRITIE KVMDSPWFKK QATRSRNEPV AATITTTEED
310 320 330 340 350
VDFLVHKSKE ETETLNAFHI IALSEGFDLS PLFEEKKKEE KREMRFATSR
360 370 380 390 400
PASSVISSLE EAARVGNKFD VRKSESRVRI EGKQNGRKGK LAVEAEIFAV
410 420 430 440
APSFVVVEVK KDHGDTLEYN NFCSTALRPA LKDIFWTSTP A
Length:441
Mass (Da):49,357
Last modified:August 1, 1998 - v1
Checksum:i469728C6CB83F428
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285106 mRNA. Translation: AAF86505.1.
AF339145 mRNA. Translation: AAK26843.1.
AL022198 Genomic DNA. Translation: CAA18197.1.
AL161578 Genomic DNA. Translation: CAB79814.1.
CP002687 Genomic DNA. Translation: AEE85835.1.
AF436831 mRNA. Translation: AAL32013.1.
AY035046 mRNA. Translation: AAK59551.1.
AY051051 mRNA. Translation: AAK93728.1.
PIRiE85362.
RefSeqiNP_194825.1. NM_119244.2.
UniGeneiAt.2154.

Genome annotation databases

EnsemblPlantsiAT4G30960.1; AT4G30960.1; AT4G30960.
GeneIDi829221.
GrameneiAT4G30960.1; AT4G30960.1; AT4G30960.
KEGGiath:AT4G30960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285106 mRNA. Translation: AAF86505.1.
AF339145 mRNA. Translation: AAK26843.1.
AL022198 Genomic DNA. Translation: CAA18197.1.
AL161578 Genomic DNA. Translation: CAB79814.1.
CP002687 Genomic DNA. Translation: AEE85835.1.
AF436831 mRNA. Translation: AAL32013.1.
AY035046 mRNA. Translation: AAK59551.1.
AY051051 mRNA. Translation: AAK93728.1.
PIRiE85362.
RefSeqiNP_194825.1. NM_119244.2.
UniGeneiAt.2154.

3D structure databases

ProteinModelPortaliO65554.
SMRiO65554. Positions 22-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14508. 11 interactions.
DIPiDIP-34749N.
IntActiO65554. 12 interactions.
STRINGi3702.AT4G30960.1.

PTM databases

iPTMnetiO65554.

Proteomic databases

PaxDbiO65554.
PRIDEiO65554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30960.1; AT4G30960.1; AT4G30960.
GeneIDi829221.
GrameneiAT4G30960.1; AT4G30960.1; AT4G30960.
KEGGiath:AT4G30960.

Organism-specific databases

TAIRiAT4G30960.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO65554.
OMAiISKVMDS.
OrthoDBiEOG09360BAB.
PhylomeDBiO65554.

Enzyme and pathway databases

BioCyciARA:AT4G30960-MONOMER.

Miscellaneous databases

PROiO65554.

Gene expression databases

GenevisibleiO65554. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIPK6_ARATH
AccessioniPrimary (citable) accession number: O65554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.