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Protein

Cysteine protease XCP1

Gene

XCP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cysteine protease involved in xylem tracheary element (TE) autolysis during xylogenesis in roots. Participates in micro autolysis within the intact central vacuole before mega autolysis is initiated by tonoplast implosion.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611PROSITE-ProRule annotation
Active sitei297 – 2971PROSITE-ProRule annotation
Active sitei317 – 3171PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • programmed cell death involved in cell development Source: TAIR
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciARA:AT4G35350-MONOMER.
ARA:GQT-1554-MONOMER.

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine protease XCP1Curated (EC:3.4.22.-)
Alternative name(s):
Xylem cysteine peptidase 11 Publication
Short name:
AtXCP11 Publication
Gene namesi
Name:XCP11 Publication
Ordered Locus Names:At4g35350
ORF Names:F23E12.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G35350.

Subcellular locationi

  • Vacuole 1 Publication
  • Cell membrane 1 Publication

  • Note: Predominantly vacuolar. May be associated to plasma membrane.

GO - Cellular componenti

  • extracellular space Source: GO_Central
  • lysosome Source: GO_Central
  • nucleus Source: TAIR
  • plant-type vacuole Source: TAIR
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Propeptidei29 – 136108Activation peptideCuratedPRO_0000026467Add
BLAST
Chaini137 – 355219Cysteine protease XCP1PRO_0000026468Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi158 ↔ 200By similarity
Glycosylationi180 – 1801N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi192 ↔ 233By similarity
Disulfide bondi291 ↔ 342By similarity

Post-translational modificationi

Autocleaves.

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO65493.
PRIDEiO65493.

Expressioni

Tissue specificityi

Mostly expressed in roots, stems and flowers. Confined to tracheary elements, and specifically to xylem.2 Publications

Gene expression databases

ExpressionAtlasiO65493. baseline and differential.
GenevisibleiO65493. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G35350.1.

Structurei

3D structure databases

ProteinModelPortaliO65493.
SMRiO65493. Positions 41-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230773.
InParanoidiO65493.
KOiK16290.
OMAiKEVTSYW.
OrthoDBiEOG09360E3O.
PhylomeDBiO65493.

Family and domain databases

InterProiIPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O65493-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFSAPSLSK FSLLVAISAS ALLCCAFARD FSIVGYTPEH LTNTDKLLEL
60 70 80 90 100
FESWMSEHSK AYKSVEEKVH RFEVFRENLM HIDQRNNEIN SYWLGLNEFA
110 120 130 140 150
DLTHEEFKGR YLGLAKPQFS RKRQPSANFR YRDITDLPKS VDWRKKGAVA
160 170 180 190 200
PVKDQGQCGS CWAFSTVAAV EGINQITTGN LSSLSEQELI DCDTTFNSGC
210 220 230 240 250
NGGLMDYAFQ YIISTGGLHK EDDYPYLMEE GICQEQKEDV ERVTISGYED
260 270 280 290 300
VPENDDESLV KALAHQPVSV AIEASGRDFQ FYKGGVFNGK CGTDLDHGVA
310 320 330 340 350
AVGYGSSKGS DYVIVKNSWG PRWGEKGFIR MKRNTGKPEG LCGINKMASY

PTKTK
Length:355
Mass (Da):39,618
Last modified:August 1, 1998 - v1
Checksum:i0EB501744967A427
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191027 mRNA. Translation: AAF25831.1.
AL022604 Genomic DNA. Translation: CAA18734.1.
AL161587 Genomic DNA. Translation: CAB80252.1.
CP002687 Genomic DNA. Translation: AEE86501.1.
AK117394 mRNA. Translation: BAC42063.1.
BT005179 mRNA. Translation: AAO50712.1.
PIRiT06122.
RefSeqiNP_567983.1. NM_119701.3. [O65493-1]
UniGeneiAt.2280.
At.67622.

Genome annotation databases

EnsemblPlantsiAT4G35350.1; AT4G35350.1; AT4G35350. [O65493-1]
GeneIDi829688.
KEGGiath:AT4G35350.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191027 mRNA. Translation: AAF25831.1.
AL022604 Genomic DNA. Translation: CAA18734.1.
AL161587 Genomic DNA. Translation: CAB80252.1.
CP002687 Genomic DNA. Translation: AEE86501.1.
AK117394 mRNA. Translation: BAC42063.1.
BT005179 mRNA. Translation: AAO50712.1.
PIRiT06122.
RefSeqiNP_567983.1. NM_119701.3. [O65493-1]
UniGeneiAt.2280.
At.67622.

3D structure databases

ProteinModelPortaliO65493.
SMRiO65493. Positions 41-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G35350.1.

Protein family/group databases

MEROPSiI29.003.

Proteomic databases

PaxDbiO65493.
PRIDEiO65493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G35350.1; AT4G35350.1; AT4G35350. [O65493-1]
GeneIDi829688.
KEGGiath:AT4G35350.

Organism-specific databases

TAIRiAT4G35350.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230773.
InParanoidiO65493.
KOiK16290.
OMAiKEVTSYW.
OrthoDBiEOG09360E3O.
PhylomeDBiO65493.

Enzyme and pathway databases

BioCyciARA:AT4G35350-MONOMER.
ARA:GQT-1554-MONOMER.

Miscellaneous databases

PROiO65493.

Gene expression databases

ExpressionAtlasiO65493. baseline and differential.
GenevisibleiO65493. AT.

Family and domain databases

InterProiIPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXCP1_ARATH
AccessioniPrimary (citable) accession number: O65493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.