ID   CRK9_ARATH              Reviewed;         265 AA.
AC   O65469;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   27-MAR-2024, entry version 119.
DE   RecName: Full=Putative cysteine-rich receptor-like protein kinase 9;
DE            Short=Cysteine-rich RLK9;
DE   Flags: Precursor;
GN   Name=CRK9; Synonyms=EP1; OrderedLocusNames=At4g23170;
GN   ORFNames=F21P8.60;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX   PubMed=11402176; DOI=10.1104/pp.126.2.473;
RA   Chen Z.;
RT   "A superfamily of proteins with novel cysteine-rich repeats.";
RL   Plant Physiol. 126:473-476(2001).
RN   [5]
RP   INDUCTION.
RX   PubMed=16307367; DOI=10.1007/s11103-005-2227-x;
RA   Blanco F., Garreton V., Frey N., Dominguez C., Perez-Acle T.,
RA   van der Straeten D., Jordana X., Holuigue L.;
RT   "Identification of NPR1-dependent and independent genes early induced by
RT   salicylic acid treatment in Arabidopsis.";
RL   Plant Mol. Biol. 59:927-944(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- INDUCTION: By salicylic acid (SA). {ECO:0000269|PubMed:16307367}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. CRK subfamily. {ECO:0000305}.
CC   -!- CAUTION: Lacks the transmembrane and the protein kinase domains, which
CC       are conserved features of the CRK subfamily. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
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DR   EMBL; AL022347; CAA18464.1; -; Genomic_DNA.
DR   EMBL; AL161558; CAB79272.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE84718.1; -; Genomic_DNA.
DR   EMBL; BT002377; AAO00737.1; -; mRNA.
DR   EMBL; BT006299; AAP13407.1; -; mRNA.
DR   PIR; T04834; T04834.
DR   RefSeq; NP_194048.1; NM_118446.3.
DR   AlphaFoldDB; O65469; -.
DR   SMR; O65469; -.
DR   BioGRID; 13705; 8.
DR   IntAct; O65469; 8.
DR   STRING; 3702.O65469; -.
DR   GlyCosmos; O65469; 6 sites, No reported glycans.
DR   iPTMnet; O65469; -.
DR   PaxDb; 3702-AT4G23170-1; -.
DR   ProteomicsDB; 222769; -.
DR   EnsemblPlants; AT4G23170.1; AT4G23170.1; AT4G23170.
DR   GeneID; 828416; -.
DR   Gramene; AT4G23170.1; AT4G23170.1; AT4G23170.
DR   KEGG; ath:AT4G23170; -.
DR   Araport; AT4G23170; -.
DR   TAIR; AT4G23170; EP1.
DR   eggNOG; ENOG502QWDY; Eukaryota.
DR   HOGENOM; CLU_000288_35_0_1; -.
DR   InParanoid; O65469; -.
DR   OMA; SGHNEPE; -.
DR   OrthoDB; 404740at2759; -.
DR   PhylomeDB; O65469; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; O65469; baseline and differential.
DR   GO; GO:0048046; C:apoplast; HDA:TAIR.
DR   GO; GO:0099503; C:secretory vesicle; HDA:TAIR.
DR   GO; GO:0012501; P:programmed cell death; IMP:TAIR.
DR   GO; GO:0009751; P:response to salicylic acid; IEP:TAIR.
DR   GO; GO:0009627; P:systemic acquired resistance; IEP:TAIR.
DR   Gene3D; 3.30.430.20; Gnk2 domain, C-X8-C-X2-C motif; 2.
DR   InterPro; IPR002902; GNK2.
DR   InterPro; IPR038408; GNK2_sf.
DR   PANTHER; PTHR32099:SF42; CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 9-RELATED; 1.
DR   PANTHER; PTHR32099; CYSTEINE-RICH REPEAT SECRETORY PROTEIN; 1.
DR   Pfam; PF01657; Stress-antifung; 2.
DR   PROSITE; PS51473; GNK2; 2.
PE   5: Uncertain;
KW   Glycoprotein; Receptor; Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..265
FT                   /note="Putative cysteine-rich receptor-like protein kinase
FT                   9"
FT                   /id="PRO_0000295056"
FT   DOMAIN          27..131
FT                   /note="Gnk2-homologous 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DOMAIN          142..244
FT                   /note="Gnk2-homologous 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   CARBOHYD        35
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        60
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        177
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        246
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   265 AA;  29722 MW;  0404A61E511031F4 CRC64;
     MSSLISFIFL FLFSFLTSFK ASAQDPFYLN HYCPNTTTYS RFSTYSTNLR TLLSSFASRN
     ASYSTGFQNV TVGQTPDLVT GLFLCRGDLS PEVCSNCVAF SVDEALTRCP SQREAVFYYE
     ECILRYSDKN ILSTAITNEG EFILSNTNTI SPNQSQINQF IVLVQSNMNQ AAMEAANSSR
     KFSTIKTELT ELQTLYGLVQ CTPDLTSQDC LRCLTRSINR MPLSRIGARQ FWPSCNSRYE
     LYSFYNETTI GTPSPPPPPS PSRAN
//