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Protein

Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3

Gene

BAM3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for male gametophyte development, as well as ovule specification and function. Required for the development of high-ordered vascular strands within the leaf and a correlated control of leaf shape, size and symmetry.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei738ATPPROSITE-ProRule annotation1
Active sitei836Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi716 – 724ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • floral organ development Source: TAIR
  • regulation of meristem growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3 (EC:2.7.11.1)
Alternative name(s):
Protein BARELY ANY MERISTEM 3
Gene namesi
Name:BAM3
Ordered Locus Names:At4g20270
ORF Names:F1C12.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G20270.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 656ExtracellularSequence analysisAdd BLAST635
Transmembranei657 – 677HelicalSequence analysisAdd BLAST21
Topological domaini678 – 992CytoplasmicSequence analysisAdd BLAST315

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

When associated with BAM1 and BAM2 disruptions, loss of stem cells at the shoot and flower meristems.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000040335422 – 992Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3Add BLAST971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi87N-linked (GlcNAc...)Sequence analysis1
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Glycosylationi256N-linked (GlcNAc...)Sequence analysis1
Glycosylationi293N-linked (GlcNAc...)Sequence analysis1
Glycosylationi354N-linked (GlcNAc...)Sequence analysis1
Glycosylationi440N-linked (GlcNAc...)Sequence analysis1
Glycosylationi472N-linked (GlcNAc...)Sequence analysis1
Glycosylationi525N-linked (GlcNAc...)Sequence analysis1
Glycosylationi568N-linked (GlcNAc...)Sequence analysis1
Glycosylationi575N-linked (GlcNAc...)Sequence analysis1
Glycosylationi597N-linked (GlcNAc...)Sequence analysis1
Glycosylationi612N-linked (GlcNAc...)Sequence analysis1
Glycosylationi613N-linked (GlcNAc...)Sequence analysis1
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Glycosylationi635N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO65440.
PRIDEiO65440.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, leaves, stems, inflorescences, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiO65440. AT.

Interactioni

Protein-protein interaction databases

BioGridi13066. 3 interactors.
STRINGi3702.AT4G20270.1.

Structurei

3D structure databases

ProteinModelPortaliO65440.
SMRiO65440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati75 – 99LRR 1Add BLAST25
Repeati100 – 124LRR 2Add BLAST25
Repeati126 – 148LRR 3Add BLAST23
Repeati150 – 173LRR 4Add BLAST24
Repeati174 – 199LRR 5Add BLAST26
Repeati201 – 221LRR 6Add BLAST21
Repeati246 – 270LRR 7Add BLAST25
Repeati271 – 294LRR 8Add BLAST24
Repeati296 – 320LRR 9Add BLAST25
Repeati322 – 342LRR 10Add BLAST21
Repeati343 – 366LRR 11Add BLAST24
Repeati368 – 391LRR 12Add BLAST24
Repeati393 – 414LRR 13Add BLAST22
Repeati415 – 438LRR 14Add BLAST24
Repeati439 – 462LRR 15Add BLAST24
Repeati465 – 489LRR 16Add BLAST25
Repeati491 – 513LRR 17Add BLAST23
Repeati514 – 536LRR 18Add BLAST23
Repeati537 – 561LRR 19Add BLAST25
Repeati563 – 585LRR 20Add BLAST23
Repeati586 – 610LRR 21Add BLAST25
Domaini710 – 992Protein kinasePROSITE-ProRule annotationAdd BLAST283

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 21 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFVD. Eukaryota.
COG4886. LUCA.
InParanoidiO65440.
OMAiLAMCWSA.
OrthoDBiEOG0936030Y.
PhylomeDBiO65440.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 9 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 13 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O65440-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKIFTFFL ILSSISPLLC SSLISPLNLS LIRQANVLIS LKQSFDSYDP
60 70 80 90 100
SLDSWNIPNF NSLCSWTGVS CDNLNQSITR LDLSNLNISG TISPEISRLS
110 120 130 140 150
PSLVFLDISS NSFSGELPKE IYELSGLEVL NISSNVFEGE LETRGFSQMT
160 170 180 190 200
QLVTLDAYDN SFNGSLPLSL TTLTRLEHLD LGGNYFDGEI PRSYGSFLSL
210 220 230 240 250
KFLSLSGNDL RGRIPNELAN ITTLVQLYLG YYNDYRGGIP ADFGRLINLV
260 270 280 290 300
HLDLANCSLK GSIPAELGNL KNLEVLFLQT NELTGSVPRE LGNMTSLKTL
310 320 330 340 350
DLSNNFLEGE IPLELSGLQK LQLFNLFFNR LHGEIPEFVS ELPDLQILKL
360 370 380 390 400
WHNNFTGKIP SKLGSNGNLI EIDLSTNKLT GLIPESLCFG RRLKILILFN
410 420 430 440 450
NFLFGPLPED LGQCEPLWRF RLGQNFLTSK LPKGLIYLPN LSLLELQNNF
460 470 480 490 500
LTGEIPEEEA GNAQFSSLTQ INLSNNRLSG PIPGSIRNLR SLQILLLGAN
510 520 530 540 550
RLSGQIPGEI GSLKSLLKID MSRNNFSGKF PPEFGDCMSL TYLDLSHNQI
560 570 580 590 600
SGQIPVQISQ IRILNYLNVS WNSFNQSLPN ELGYMKSLTS ADFSHNNFSG
610 620 630 640 650
SVPTSGQFSY FNNTSFLGNP FLCGFSSNPC NGSQNQSQSQ LLNQNNARSR
660 670 680 690 700
GEISAKFKLF FGLGLLGFFL VFVVLAVVKN RRMRKNNPNL WKLIGFQKLG
710 720 730 740 750
FRSEHILECV KENHVIGKGG RGIVYKGVMP NGEEVAVKKL LTITKGSSHD
760 770 780 790 800
NGLAAEIQTL GRIRHRNIVR LLAFCSNKDV NLLVYEYMPN GSLGEVLHGK
810 820 830 840 850
AGVFLKWETR LQIALEAAKG LCYLHHDCSP LIIHRDVKSN NILLGPEFEA
860 870 880 890 900
HVADFGLAKF MMQDNGASEC MSSIAGSYGY IAPEYAYTLR IDEKSDVYSF
910 920 930 940 950
GVVLLELITG RKPVDNFGEE GIDIVQWSKI QTNCNRQGVV KIIDQRLSNI
960 970 980 990
PLAEAMELFF VAMLCVQEHS VERPTMREVV QMISQAKQPN TF
Length:992
Mass (Da):110,283
Last modified:March 8, 2011 - v3
Checksum:iA394AA19376BDA20
GO
Isoform 2 (identifier: O65440-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-409: Missing.

Show »
Length:963
Mass (Da):106,983
Checksum:i59E400927B84F881
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti542Y → C in AK229453 (Ref. 4) Curated1
Sequence conflicti721R → A in ACN59341 (PubMed:20064227).Curated1
Sequence conflicti721R → A in AK229453 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040681381 – 409Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022224 Genomic DNA. Translation: CAA18252.1.
AL161552 Genomic DNA. Translation: CAB79027.1.
CP002687 Genomic DNA. Translation: AEE84297.1.
FJ708747 mRNA. Translation: ACN59341.1.
AK229453 mRNA. No translation available.
PIRiT05335.
RefSeqiNP_193760.1. NM_118146.5. [O65440-1]
UniGeneiAt.32731.

Genome annotation databases

EnsemblPlantsiAT4G20270.1; AT4G20270.1; AT4G20270. [O65440-1]
GeneIDi827774.
GrameneiAT4G20270.1; AT4G20270.1; AT4G20270.
KEGGiath:AT4G20270.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022224 Genomic DNA. Translation: CAA18252.1.
AL161552 Genomic DNA. Translation: CAB79027.1.
CP002687 Genomic DNA. Translation: AEE84297.1.
FJ708747 mRNA. Translation: ACN59341.1.
AK229453 mRNA. No translation available.
PIRiT05335.
RefSeqiNP_193760.1. NM_118146.5. [O65440-1]
UniGeneiAt.32731.

3D structure databases

ProteinModelPortaliO65440.
SMRiO65440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13066. 3 interactors.
STRINGi3702.AT4G20270.1.

Proteomic databases

PaxDbiO65440.
PRIDEiO65440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G20270.1; AT4G20270.1; AT4G20270. [O65440-1]
GeneIDi827774.
GrameneiAT4G20270.1; AT4G20270.1; AT4G20270.
KEGGiath:AT4G20270.

Organism-specific databases

TAIRiAT4G20270.

Phylogenomic databases

eggNOGiENOG410IFVD. Eukaryota.
COG4886. LUCA.
InParanoidiO65440.
OMAiLAMCWSA.
OrthoDBiEOG0936030Y.
PhylomeDBiO65440.

Miscellaneous databases

PROiO65440.

Gene expression databases

GenevisibleiO65440. AT.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 9 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 13 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAME3_ARATH
AccessioniPrimary (citable) accession number: O65440
Secondary accession number(s): C0LGQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 8, 2011
Last modified: November 30, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.