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O65399

- E131_ARATH

UniProt

O65399 - E131_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 1

Gene

At1g11820

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (16 Nov 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei284 – 2841NucleophileBy similarity
    Active sitei347 – 3471Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT1G11820-MONOMER.
    ARA:GQT-1170-MONOMER.

    Protein family/group databases

    CAZyiCBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 1 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 1
    Short name:
    (1->3)-beta-glucanase 1
    Beta-1,3-endoglucanase 1
    Short name:
    Beta-1,3-glucanase 1
    Gene namesi
    Ordered Locus Names:At1g11820, At1g11830
    ORF Names:F12F1.33, F25C20_1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G11820.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 485457Glucan endo-1,3-beta-glucosidase 1PRO_0000011884Add
    BLAST
    Propeptidei486 – 51126Removed in mature formSequence AnalysisPRO_0000011885Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi382 ↔ 445By similarity
    Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
    Lipidationi485 – 4851GPI-anchor amidated alanineSequence Analysis

    Post-translational modificationi

    Contains two additional disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiO65399.
    PRIDEiO65399.

    Expressioni

    Gene expression databases

    ArrayExpressiO65399.
    GenevestigatoriO65399.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G11820.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO65399.
    SMRiO65399. Positions 44-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG329755.
    HOGENOMiHOG000238220.
    InParanoidiO65399.
    OMAiPFVGFNI.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view]
    SMARTiSM00768. X8. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: O65399-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAFTSMVSTV PVLFFFFTLL LISANSSSLS HNIKVQEQDK DPFVGFNIGT    50
    DVSNLLSPTE LVKFLQAQKV NHVRLYDADP ELLKALAKTK VRVIISVPNN 100
    QLLAIGSSNS TAASWIGRNV VAYYPETLIT AISVGDEVLT TVPSSAPLLL 150
    PAIESLYNAL VASNLHTQIK VSTPHAASIM LDTFPPSQAY FNQTWHSIMV 200
    PLLQFLSKTG SPLMMNLYPY YVYMQNKGVV PLDNCLFEPL TPSKEMVDPN 250
    TLLHYTNVLD AMVDAAYVSM KNLNVSDVAV LVTESGWPSK GDSKEPYATI 300
    DNADTYNSNL IKHVFDRTGT PLHPEMTSSV YIYELFNEDL RAPPVSEASW 350
    GLFYGNSTPV YLLHVSGSGT FLANDTTNQT YCIAMDGVDA KTLQAALDWA 400
    CGPGRSNCSE IQPGESCYQP NNVKGHASFA FNSYYQKEGR ASGSCDFKGV 450
    AMITTTDPSH GSCIFPGSKK VGNRTQTVVN STEVAAGEAT SRSLSRGFCV 500
    TIMILVTFSI L 511
    Length:511
    Mass (Da):55,630
    Last modified:November 16, 2011 - v3
    Checksum:i1C29693D4F94E8C4
    GO

    Sequence cautioni

    The sequence AAO42272.1 differs from that shown. Reason: Probable cloning artifact leading to a deletion into the sequence.
    The sequence AAC17632.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002131 Genomic DNA. Translation: AAC17632.1. Sequence problems.
    AC007296 Genomic DNA. No translation available.
    CP002684 Genomic DNA. Translation: AEE28791.1.
    BT004271 mRNA. Translation: AAO42272.1. Sequence problems.
    PIRiE86252.
    RefSeqiNP_001184967.1. NM_001198038.1. [O65399-1]
    UniGeneiAt.10643.

    Genome annotation databases

    EnsemblPlantsiAT1G11820.2; AT1G11820.2; AT1G11820. [O65399-1]
    GeneIDi837730.
    KEGGiath:AT1G11820.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002131 Genomic DNA. Translation: AAC17632.1 . Sequence problems.
    AC007296 Genomic DNA. No translation available.
    CP002684 Genomic DNA. Translation: AEE28791.1 .
    BT004271 mRNA. Translation: AAO42272.1 . Sequence problems.
    PIRi E86252.
    RefSeqi NP_001184967.1. NM_001198038.1. [O65399-1 ]
    UniGenei At.10643.

    3D structure databases

    ProteinModelPortali O65399.
    SMRi O65399. Positions 44-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G11820.1-P.

    Protein family/group databases

    CAZyi CBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi O65399.
    PRIDEi O65399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G11820.2 ; AT1G11820.2 ; AT1G11820 . [O65399-1 ]
    GeneIDi 837730.
    KEGGi ath:AT1G11820.

    Organism-specific databases

    TAIRi AT1G11820.

    Phylogenomic databases

    eggNOGi NOG329755.
    HOGENOMi HOG000238220.
    InParanoidi O65399.
    OMAi PFVGFNI.

    Enzyme and pathway databases

    BioCyci ARA:AT1G11820-MONOMER.
    ARA:GQT-1170-MONOMER.

    Gene expression databases

    ArrayExpressi O65399.
    Genevestigatori O65399.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view ]
    SMARTi SM00768. X8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
      Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
      Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
      Tissue: Callus.

    Entry informationi

    Entry nameiE131_ARATH
    AccessioniPrimary (citable) accession number: O65399
    Secondary accession number(s): F4IAH7, Q84W37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: November 16, 2011
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3