Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucan endo-1,3-beta-glucosidase 1

Gene

At1g11820

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei284NucleophileBy similarity1
Active sitei347Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G11820-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 1 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 1
Short name:
(1->3)-beta-glucanase 1
Beta-1,3-endoglucanase 1
Short name:
Beta-1,3-glucanase 1
Gene namesi
Ordered Locus Names:At1g11820, At1g11830
ORF Names:F12F1.33, F25C20_1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G11820.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000001188429 – 485Glucan endo-1,3-beta-glucosidase 1Add BLAST457
PropeptideiPRO_0000011885486 – 511Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi192N-linked (GlcNAc...)Sequence analysis1
Glycosylationi274N-linked (GlcNAc...)Sequence analysis1
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Glycosylationi378N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi382 ↔ 445By similarity
Glycosylationi407N-linked (GlcNAc...)Sequence analysis1
Glycosylationi473N-linked (GlcNAc...)Sequence analysis1
Glycosylationi480N-linked (GlcNAc...)Sequence analysis1
Lipidationi485GPI-anchor amidated alanineSequence analysis1

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO65399.
PRIDEiO65399.

Expressioni

Gene expression databases

ExpressionAtlasiO65399. baseline and differential.
GenevisibleiO65399. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G11820.2.

Structurei

3D structure databases

ProteinModelPortaliO65399.
SMRiO65399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIBH. Eukaryota.
ENOG410YEGG. LUCA.
HOGENOMiHOG000238220.
InParanoidiO65399.
KOiK19891.
OMAiSGEPYVG.
OrthoDBiEOG093607VJ.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O65399-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFTSMVSTV PVLFFFFTLL LISANSSSLS HNIKVQEQDK DPFVGFNIGT
60 70 80 90 100
DVSNLLSPTE LVKFLQAQKV NHVRLYDADP ELLKALAKTK VRVIISVPNN
110 120 130 140 150
QLLAIGSSNS TAASWIGRNV VAYYPETLIT AISVGDEVLT TVPSSAPLLL
160 170 180 190 200
PAIESLYNAL VASNLHTQIK VSTPHAASIM LDTFPPSQAY FNQTWHSIMV
210 220 230 240 250
PLLQFLSKTG SPLMMNLYPY YVYMQNKGVV PLDNCLFEPL TPSKEMVDPN
260 270 280 290 300
TLLHYTNVLD AMVDAAYVSM KNLNVSDVAV LVTESGWPSK GDSKEPYATI
310 320 330 340 350
DNADTYNSNL IKHVFDRTGT PLHPEMTSSV YIYELFNEDL RAPPVSEASW
360 370 380 390 400
GLFYGNSTPV YLLHVSGSGT FLANDTTNQT YCIAMDGVDA KTLQAALDWA
410 420 430 440 450
CGPGRSNCSE IQPGESCYQP NNVKGHASFA FNSYYQKEGR ASGSCDFKGV
460 470 480 490 500
AMITTTDPSH GSCIFPGSKK VGNRTQTVVN STEVAAGEAT SRSLSRGFCV
510
TIMILVTFSI L
Length:511
Mass (Da):55,630
Last modified:November 16, 2011 - v3
Checksum:i1C29693D4F94E8C4
GO

Sequence cautioni

The sequence AAC17632 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAO42272 differs from that shown. Probable cloning artifact leading to a deletion into the sequence.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17632.1. Sequence problems.
AC007296 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE28791.1.
BT004271 mRNA. Translation: AAO42272.1. Sequence problems.
PIRiE86252.
RefSeqiNP_001184967.1. NM_001198038.2. [O65399-1]
UniGeneiAt.10643.

Genome annotation databases

EnsemblPlantsiAT1G11820.2; AT1G11820.2; AT1G11820. [O65399-1]
GeneIDi837730.
GrameneiAT1G11820.2; AT1G11820.2; AT1G11820.
KEGGiath:AT1G11820.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17632.1. Sequence problems.
AC007296 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE28791.1.
BT004271 mRNA. Translation: AAO42272.1. Sequence problems.
PIRiE86252.
RefSeqiNP_001184967.1. NM_001198038.2. [O65399-1]
UniGeneiAt.10643.

3D structure databases

ProteinModelPortaliO65399.
SMRiO65399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G11820.2.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiO65399.
PRIDEiO65399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G11820.2; AT1G11820.2; AT1G11820. [O65399-1]
GeneIDi837730.
GrameneiAT1G11820.2; AT1G11820.2; AT1G11820.
KEGGiath:AT1G11820.

Organism-specific databases

TAIRiAT1G11820.

Phylogenomic databases

eggNOGiENOG410IIBH. Eukaryota.
ENOG410YEGG. LUCA.
HOGENOMiHOG000238220.
InParanoidiO65399.
KOiK19891.
OMAiSGEPYVG.
OrthoDBiEOG093607VJ.

Enzyme and pathway databases

BioCyciARA:AT1G11820-MONOMER.

Miscellaneous databases

PROiO65399.

Gene expression databases

ExpressionAtlasiO65399. baseline and differential.
GenevisibleiO65399. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE131_ARATH
AccessioniPrimary (citable) accession number: O65399
Secondary accession number(s): F4IAH7, Q84W37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: November 16, 2011
Last modified: November 30, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.