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O65399

- E131_ARATH

UniProt

O65399 - E131_ARATH

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Protein

Glucan endo-1,3-beta-glucosidase 1

Gene

At1g11820

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei284 – 2841NucleophileBy similarity
Active sitei347 – 3471Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G11820-MONOMER.
ARA:GQT-1170-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 1 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 1
Short name:
(1->3)-beta-glucanase 1
Beta-1,3-endoglucanase 1
Short name:
Beta-1,3-glucanase 1
Gene namesi
Ordered Locus Names:At1g11820, At1g11830
ORF Names:F12F1.33, F25C20_1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G11820.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 485457Glucan endo-1,3-beta-glucosidase 1PRO_0000011884Add
BLAST
Propeptidei486 – 51126Removed in mature formSequence AnalysisPRO_0000011885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi382 ↔ 445By similarity
Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
Lipidationi485 – 4851GPI-anchor amidated alanineSequence Analysis

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO65399.
PRIDEiO65399.

Expressioni

Gene expression databases

ExpressionAtlasiO65399. baseline and differential.
GenevestigatoriO65399.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G11820.1-P.

Structurei

3D structure databases

ProteinModelPortaliO65399.
SMRiO65399. Positions 44-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG329755.
HOGENOMiHOG000238220.
InParanoidiO65399.
OMAiPFVGFNI.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O65399-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFTSMVSTV PVLFFFFTLL LISANSSSLS HNIKVQEQDK DPFVGFNIGT
60 70 80 90 100
DVSNLLSPTE LVKFLQAQKV NHVRLYDADP ELLKALAKTK VRVIISVPNN
110 120 130 140 150
QLLAIGSSNS TAASWIGRNV VAYYPETLIT AISVGDEVLT TVPSSAPLLL
160 170 180 190 200
PAIESLYNAL VASNLHTQIK VSTPHAASIM LDTFPPSQAY FNQTWHSIMV
210 220 230 240 250
PLLQFLSKTG SPLMMNLYPY YVYMQNKGVV PLDNCLFEPL TPSKEMVDPN
260 270 280 290 300
TLLHYTNVLD AMVDAAYVSM KNLNVSDVAV LVTESGWPSK GDSKEPYATI
310 320 330 340 350
DNADTYNSNL IKHVFDRTGT PLHPEMTSSV YIYELFNEDL RAPPVSEASW
360 370 380 390 400
GLFYGNSTPV YLLHVSGSGT FLANDTTNQT YCIAMDGVDA KTLQAALDWA
410 420 430 440 450
CGPGRSNCSE IQPGESCYQP NNVKGHASFA FNSYYQKEGR ASGSCDFKGV
460 470 480 490 500
AMITTTDPSH GSCIFPGSKK VGNRTQTVVN STEVAAGEAT SRSLSRGFCV
510
TIMILVTFSI L
Length:511
Mass (Da):55,630
Last modified:November 16, 2011 - v3
Checksum:i1C29693D4F94E8C4
GO

Sequence cautioni

The sequence AAC17632.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAO42272.1 differs from that shown. Reason: Probable cloning artifact leading to a deletion into the sequence.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17632.1. Sequence problems.
AC007296 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE28791.1.
BT004271 mRNA. Translation: AAO42272.1. Sequence problems.
PIRiE86252.
RefSeqiNP_001184967.1. NM_001198038.1. [O65399-1]
UniGeneiAt.10643.

Genome annotation databases

EnsemblPlantsiAT1G11820.2; AT1G11820.2; AT1G11820. [O65399-1]
GeneIDi837730.
KEGGiath:AT1G11820.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17632.1 . Sequence problems.
AC007296 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE28791.1 .
BT004271 mRNA. Translation: AAO42272.1 . Sequence problems.
PIRi E86252.
RefSeqi NP_001184967.1. NM_001198038.1. [O65399-1 ]
UniGenei At.10643.

3D structure databases

ProteinModelPortali O65399.
SMRi O65399. Positions 44-361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G11820.1-P.

Protein family/group databases

CAZyi CBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbi O65399.
PRIDEi O65399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G11820.2 ; AT1G11820.2 ; AT1G11820 . [O65399-1 ]
GeneIDi 837730.
KEGGi ath:AT1G11820.

Organism-specific databases

TAIRi AT1G11820.

Phylogenomic databases

eggNOGi NOG329755.
HOGENOMi HOG000238220.
InParanoidi O65399.
OMAi PFVGFNI.

Enzyme and pathway databases

BioCyci ARA:AT1G11820-MONOMER.
ARA:GQT-1170-MONOMER.

Gene expression databases

ExpressionAtlasi O65399. baseline and differential.
Genevestigatori O65399.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view ]
SMARTi SM00768. X8. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
    Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
    Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
    Tissue: Callus.

Entry informationi

Entry nameiE131_ARATH
AccessioniPrimary (citable) accession number: O65399
Secondary accession number(s): F4IAH7, Q84W37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: November 16, 2011
Last modified: October 29, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3