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Protein

Aminomethyltransferase, mitochondrial

Gene

GDCST

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The glycine decarboxylase (GDC) or glycine cleavage system catalyzes the degradation of glycine.By similarity

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei235 – 2351SubstrateBy similarity
Binding sitei266 – 2661SubstrateBy similarity
Binding sitei404 – 4041SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycine catabolic process Source: InterPro
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G11860-MONOMER.
ARA:GQT-2332-MONOMER.
ARA:GQT-2333-MONOMER.
ReactomeiR-ATH-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminomethyltransferase, mitochondrial (EC:2.1.2.10)
Alternative name(s):
Glycine cleavage system T protein
Short name:
GCVT
Gene namesi
Name:GDCST
Synonyms:GDT1
Ordered Locus Names:At1g11860
ORF Names:F12F1.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G11860.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • cytosolic ribosome Source: TAIR
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424MitochondrionSequence analysisAdd
BLAST
Chaini25 – 408384Aminomethyltransferase, mitochondrialPRO_0000010758Add
BLAST

Proteomic databases

PaxDbiO65396.
PRIDEiO65396.

2D gel databases

SWISS-2DPAGEO65396.

PTM databases

iPTMnetiO65396.

Expressioni

Gene expression databases

GenevisibleiO65396. AT.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

BioGridi22973. 2 interactions.
STRINGi3702.AT1G11860.1.

Structurei

3D structure databases

ProteinModelPortaliO65396.
SMRiO65396. Positions 36-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2770. Eukaryota.
COG0404. LUCA.
HOGENOMiHOG000239380.
InParanoidiO65396.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG09360A95.
PhylomeDBiO65396.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65396-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGGSLWQLG QSITRRLAQS DKKVVSRRYF ASEADLKKTA LYDFHVAHGG
60 70 80 90 100
KMVPFAGWSM PIQYKDSIMD STVNCRENGS LFDVAHMCGL SLKGKDCVPF
110 120 130 140 150
LETLVVADVA GLAPGTGSLT VFTNEKGGAI DDSVITKVTD EHIYLVVNAG
160 170 180 190 200
CRDKDLAHIE EHMKAFKSKG GDVSWHIHDE RSLLALQGPL AAPVLQHLTK
210 220 230 240 250
EDLSKLYFGN FQILDINGST CFLTRTGYTG EDGFEISVPD EHAVDLAKAI
260 270 280 290 300
LEKSEGKVRL TGLGARDSLR LEAGLCLYGN DMEQHISPVE AGLTWAIGKR
310 320 330 340 350
RRAEGGFLGA DVILQQLKDG PTIRRVGFFS SGPPARSHSE VHDESGNKIG
360 370 380 390 400
EITSGGFSPN LKKNIAMGYV KSGQHKTGTK VKILVRGKPY EGSITKMPFV

ATKYYKPT
Length:408
Mass (Da):44,445
Last modified:August 1, 1998 - v1
Checksum:i93B909768912736B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17627.1.
CP002684 Genomic DNA. Translation: AEE28802.1.
CP002684 Genomic DNA. Translation: AEE28803.1.
CP002684 Genomic DNA. Translation: AEE28804.1.
AY125509 mRNA. Translation: AAM78101.1.
BT006361 mRNA. Translation: AAP21169.1.
Z26545 mRNA. Translation: CAA81316.1.
PIRiH86252.
RefSeqiNP_001184969.1. NM_001198040.1.
NP_172650.1. NM_101057.4.
NP_849646.1. NM_179315.1.
UniGeneiAt.47506.

Genome annotation databases

EnsemblPlantsiAT1G11860.1; AT1G11860.1; AT1G11860.
AT1G11860.2; AT1G11860.2; AT1G11860.
AT1G11860.3; AT1G11860.3; AT1G11860.
GeneIDi837733.
GrameneiAT1G11860.1; AT1G11860.1; AT1G11860.
AT1G11860.2; AT1G11860.2; AT1G11860.
AT1G11860.3; AT1G11860.3; AT1G11860.
KEGGiath:AT1G11860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17627.1.
CP002684 Genomic DNA. Translation: AEE28802.1.
CP002684 Genomic DNA. Translation: AEE28803.1.
CP002684 Genomic DNA. Translation: AEE28804.1.
AY125509 mRNA. Translation: AAM78101.1.
BT006361 mRNA. Translation: AAP21169.1.
Z26545 mRNA. Translation: CAA81316.1.
PIRiH86252.
RefSeqiNP_001184969.1. NM_001198040.1.
NP_172650.1. NM_101057.4.
NP_849646.1. NM_179315.1.
UniGeneiAt.47506.

3D structure databases

ProteinModelPortaliO65396.
SMRiO65396. Positions 36-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22973. 2 interactions.
STRINGi3702.AT1G11860.1.

PTM databases

iPTMnetiO65396.

2D gel databases

SWISS-2DPAGEO65396.

Proteomic databases

PaxDbiO65396.
PRIDEiO65396.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G11860.1; AT1G11860.1; AT1G11860.
AT1G11860.2; AT1G11860.2; AT1G11860.
AT1G11860.3; AT1G11860.3; AT1G11860.
GeneIDi837733.
GrameneiAT1G11860.1; AT1G11860.1; AT1G11860.
AT1G11860.2; AT1G11860.2; AT1G11860.
AT1G11860.3; AT1G11860.3; AT1G11860.
KEGGiath:AT1G11860.

Organism-specific databases

TAIRiAT1G11860.

Phylogenomic databases

eggNOGiKOG2770. Eukaryota.
COG0404. LUCA.
HOGENOMiHOG000239380.
InParanoidiO65396.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG09360A95.
PhylomeDBiO65396.

Enzyme and pathway databases

BioCyciARA:AT1G11860-MONOMER.
ARA:GQT-2332-MONOMER.
ARA:GQT-2333-MONOMER.
ReactomeiR-ATH-6783984. Glycine degradation.

Miscellaneous databases

PROiO65396.

Gene expression databases

GenevisibleiO65396. AT.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCST_ARATH
AccessioniPrimary (citable) accession number: O65396
Secondary accession number(s): Q42123
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.