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Reviewed, UniProtKB/Swiss-Prot O65396 (GCST_ARATH)

Last modified February 9, 2010. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aminomethyltransferase, mitochondrial
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein
      Short name=GCVT
Gene names
Name: GDCST
Ordered Locus Names: At1g11860
ORF Names: F12F1.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine By similarity.

Catalytic activity

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the gcvT family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Potential
Chain25 – 408384Aminomethyltransferase, mitochondrial
PRO_0000010758

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Sequences

Sequence LengthMass (Da)Tools
O65396-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 93B909768912736B

FASTA40844,445
        10         20         30         40         50         60 
MRGGSLWQLG QSITRRLAQS DKKVVSRRYF ASEADLKKTA LYDFHVAHGG KMVPFAGWSM 

        70         80         90        100        110        120 
PIQYKDSIMD STVNCRENGS LFDVAHMCGL SLKGKDCVPF LETLVVADVA GLAPGTGSLT 

       130        140        150        160        170        180 
VFTNEKGGAI DDSVITKVTD EHIYLVVNAG CRDKDLAHIE EHMKAFKSKG GDVSWHIHDE 

       190        200        210        220        230        240 
RSLLALQGPL AAPVLQHLTK EDLSKLYFGN FQILDINGST CFLTRTGYTG EDGFEISVPD 

       250        260        270        280        290        300 
EHAVDLAKAI LEKSEGKVRL TGLGARDSLR LEAGLCLYGN DMEQHISPVE AGLTWAIGKR 

       310        320        330        340        350        360 
RRAEGGFLGA DVILQQLKDG PTIRRVGFFS SGPPARSHSE VHDESGNKIG EITSGGFSPN 

       370        380        390        400 
LKKNIAMGYV KSGQHKTGTK VKILVRGKPY EGSITKMPFV ATKYYKPT

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References

[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[3]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 338-408.
Strain: cv. Columbia.
Tissue: Seedling.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC002131 Genomic DNA. Translation: AAC17627.1.
AY125509 mRNA. Translation: AAM78101.1.
BT006361 mRNA. Translation: AAP21169.1.
Z26545 mRNA. Translation: CAA81316.1.
IPIIPI00538278.
PIRH86252.
RefSeqNP_172650.1.
NP_849646.1.
UniGeneAt.47506
Rra.5045
Rsa.8447

3D structure databases

SMRO65396. Positions 36-406.
ModBaseSearch...

Protein-protein interaction databases

STRINGO65396.

2-D gel databases

SWISS-2DPAGEO65396.

Proteomic databases

PRIDEO65396.
ProMEXO65396.

Genome annotation databases

GeneID837733.
GenomeReviewsGene locus AT1G11860 in contig CT485782_GR.
KEGGath:AT1G11860.
NMPDRfig|3702.1.peg.1445.

Organism-specific databases

GeneFarm1742.
TAIRAt1g11860.

Phylogenomic databases

eggNOGKOG2770.
HOGENOMHBG299834.
InParanoidO65396.
OMAMPVQYPL.
PhylomeDBO65396.

Enzyme and pathway databases

BRENDA2.1.2.10. 302.

Gene expression databases

ArrayExpressO65396.
GenevestigatorO65396.
GermOnlineAT1G11860. Arabidopsis thaliana.

Family and domain databases

InterProIPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR006223. GcvT.
[Graphical view]
PfamPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFPIRSF006487. GcvT. 1 hit.
TIGRFAMsTIGR00528. gcvT. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGCST_ARATH
AccessionPrimary (citable) accession number: O65396
Secondary accession number(s): Q42123
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: February 9, 2010
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents