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O65396 (GCST_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aminomethyltransferase, mitochondrial
EC=2.1.2.10
Alternative name(s):
Glycine cleavage system T protein
Short name=GCVT
Gene names
Name:GDCST
Ordered Locus Names:At1g11860
ORF Names:F12F1.30
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine By similarity.

Catalytic activity

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the GcvT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Potential
Chain25 – 408384Aminomethyltransferase, mitochondrial
PRO_0000010758

Sequences

Sequence LengthMass (Da)Tools
O65396 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 93B909768912736B

FASTA40844,445
        10         20         30         40         50         60 
MRGGSLWQLG QSITRRLAQS DKKVVSRRYF ASEADLKKTA LYDFHVAHGG KMVPFAGWSM 

        70         80         90        100        110        120 
PIQYKDSIMD STVNCRENGS LFDVAHMCGL SLKGKDCVPF LETLVVADVA GLAPGTGSLT 

       130        140        150        160        170        180 
VFTNEKGGAI DDSVITKVTD EHIYLVVNAG CRDKDLAHIE EHMKAFKSKG GDVSWHIHDE 

       190        200        210        220        230        240 
RSLLALQGPL AAPVLQHLTK EDLSKLYFGN FQILDINGST CFLTRTGYTG EDGFEISVPD 

       250        260        270        280        290        300 
EHAVDLAKAI LEKSEGKVRL TGLGARDSLR LEAGLCLYGN DMEQHISPVE AGLTWAIGKR 

       310        320        330        340        350        360 
RRAEGGFLGA DVILQQLKDG PTIRRVGFFS SGPPARSHSE VHDESGNKIG EITSGGFSPN 

       370        380        390        400 
LKKNIAMGYV KSGQHKTGTK VKILVRGKPY EGSITKMPFV ATKYYKPT

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 338-408.
Strain: cv. Columbia.
Tissue: Seedling.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC002131 Genomic DNA. Translation: AAC17627.1.
CP002684 Genomic DNA. Translation: AEE28802.1.
CP002684 Genomic DNA. Translation: AEE28803.1.
CP002684 Genomic DNA. Translation: AEE28804.1.
AY125509 mRNA. Translation: AAM78101.1.
BT006361 mRNA. Translation: AAP21169.1.
Z26545 mRNA. Translation: CAA81316.1.
IPIIPI00538278.
PIRH86252.
RefSeqNP_001184969.1. NM_001198040.1.
NP_172650.1. NM_101057.4.
NP_849646.1. NM_179315.1.
UniGeneAt.47506.

3D structure databases

ProteinModelPortalO65396.
SMRO65396. Positions 36-406.
ModBaseSearch...

Protein-protein interaction databases

STRINGO65396.

2D gel databases

SWISS-2DPAGEO65396.

Proteomic databases

PRIDEO65396.
ProMEXO65396.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G11860.1; AT1G11860.1; AT1G11860.
AT1G11860.2; AT1G11860.2; AT1G11860.
AT1G11860.3; AT1G11860.3; AT1G11860.
GeneID837733.
GenomeReviewsGene locus AT1G11860 in contig CT485782_GR.
KEGGath:AT1G11860.
NMPDRfig|3702.1.peg.1445.

Organism-specific databases

GeneFarm1742.
TAIRAt1g11860.

Phylogenomic databases

eggNOGKOG2770.
GeneTreeEPGT00050000007714.
HOGENOMHBG299834.
InParanoidO65396.
OMARITRCGY.
PhylomeDBO65396.
ProtClustDBPLN02319.

Gene expression databases

ArrayExpressO65396.
GenevestigatorO65396.
GermOnlineAT1G11860. Arabidopsis thaliana.

Family and domain databases

InterProIPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR006223. GcvT.
[Graphical view]
KOK00605.
PANTHERPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFPIRSF006487. GcvT. 1 hit.
TIGRFAMsTIGR00528. GcvT. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCST_ARATH
AccessionPrimary (citable) accession number: O65396
Secondary accession number(s): Q42123
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents