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Protein

Aspartic proteinase A1

Gene

APA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the breakdown of propeptides of storage proteins in protein-storage vacuoles (By similarity). Possesses aspartic protease activity in vitro.By similarity1 Publication

pH dependencei

Optimum pH is 5.0-6.0 with insulin B chain as substrate and at 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei100 – 1001PROSITE-ProRule annotation
Active sitei287 – 2871PROSITE-ProRule annotation

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • endopeptidase activity Source: TAIR

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • protein catabolic process Source: GO_Central
  • proteolysis Source: TAIR
  • response to cytokinin Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT1G11910-MONOMER.
ReactomeiR-ATH-2132295. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase A1 (EC:3.4.23.-)
Gene namesi
Name:APA1
Synonyms:PRA1
Ordered Locus Names:At1g11910
ORF Names:F12F1.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G11910.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 6440Activation peptideSequence analysisPRO_0000420625Add
BLAST
Chaini65 – 506442Aspartic proteinase A1PRO_0000420626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi113 ↔ 119PROSITE-ProRule annotation
Disulfide bondi278 ↔ 282PROSITE-ProRule annotation
Disulfide bondi317 ↔ 411PROSITE-ProRule annotation
Disulfide bondi342 ↔ 383PROSITE-ProRule annotation
Disulfide bondi348 ↔ 380PROSITE-ProRule annotation
Glycosylationi397 – 3971N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi425 ↔ 462PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO65390.
PRIDEiO65390.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, petals, carpels, seed pods and dry seeds.1 Publication

Inductioni

By light and during senescence.2 Publications

Gene expression databases

GenevisibleiO65390. AT.

Interactioni

Protein-protein interaction databases

BioGridi22980. 12 interactions.
IntActiO65390. 11 interactions.
STRINGi3702.AT1G11910.1.

Structurei

3D structure databases

ProteinModelPortaliO65390.
SMRiO65390. Positions 32-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 503422Peptidase A1PROSITE-ProRule annotationAdd
BLAST
Domaini312 – 417106Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation
Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiO65390.
KOiK08245.
OMAiKVERQVF.
OrthoDBiEOG093606Q0.
PhylomeDBiO65390.

Family and domain databases

Gene3Di1.10.225.10. 2 hits.
2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIYSRTVAV SLIVSFLLCF SAFAERNDGT FRVGLKKLKL DSKNRLAARV
60 70 80 90 100
ESKQEKPLRA YRLGDSGDAD VVVLKNYLDA QYYGEIAIGT PPQKFTVVFD
110 120 130 140 150
TGSSNLWVPS SKCYFSLACL LHPKYKSSRS STYEKNGKAA AIHYGTGAIA
160 170 180 190 200
GFFSNDAVTV GDLVVKDQEF IEATKEPGIT FVVAKFDGIL GLGFQEISVG
210 220 230 240 250
KAAPVWYNML KQGLIKEPVF SFWLNRNADE EEGGELVFGG VDPNHFKGKH
260 270 280 290 300
TYVPVTQKGY WQFDMGDVLI GGAPTGFCES GCSAIADSGT SLLAGPTTII
310 320 330 340 350
TMINHAIGAA GVVSQQCKTV VDQYGQTILD LLLSETQPKK ICSQIGLCTF
360 370 380 390 400
DGTRGVSMGI ESVVDKENAK LSNGVGDAAC SACEMAVVWI QSQLRQNMTQ
410 420 430 440 450
ERILNYVNEL CERLPSPMGE SAVDCAQLST MPTVSLTIGG KVFDLAPEEY
460 470 480 490 500
VLKVGEGPVA QCISGFIALD VAPPRGPLWI LGDVFMGKYH TVFDFGNEQV

GFAEAA
Length:506
Mass (Da):54,614
Last modified:August 1, 1998 - v1
Checksum:i171E16AA7A0D6FA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17620.1.
CP002684 Genomic DNA. Translation: AEE28813.1.
AY056387 mRNA. Translation: AAL08243.1.
AY056403 mRNA. Translation: AAL08259.1.
AY063974 mRNA. Translation: AAL36330.1.
BT001980 mRNA. Translation: AAN71979.1.
AY088657 mRNA. Translation: AAM66979.1.
U51036 mRNA. Translation: AAC49730.1.
PIRiF86253.
RefSeqiNP_172655.1. NM_101062.3.
UniGeneiAt.24544.
At.67307.

Genome annotation databases

EnsemblPlantsiAT1G11910.1; AT1G11910.1; AT1G11910.
GeneIDi837740.
GrameneiAT1G11910.1; AT1G11910.1; AT1G11910.
KEGGiath:AT1G11910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA. Translation: AAC17620.1.
CP002684 Genomic DNA. Translation: AEE28813.1.
AY056387 mRNA. Translation: AAL08243.1.
AY056403 mRNA. Translation: AAL08259.1.
AY063974 mRNA. Translation: AAL36330.1.
BT001980 mRNA. Translation: AAN71979.1.
AY088657 mRNA. Translation: AAM66979.1.
U51036 mRNA. Translation: AAC49730.1.
PIRiF86253.
RefSeqiNP_172655.1. NM_101062.3.
UniGeneiAt.24544.
At.67307.

3D structure databases

ProteinModelPortaliO65390.
SMRiO65390. Positions 32-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22980. 12 interactions.
IntActiO65390. 11 interactions.
STRINGi3702.AT1G11910.1.

Proteomic databases

PaxDbiO65390.
PRIDEiO65390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G11910.1; AT1G11910.1; AT1G11910.
GeneIDi837740.
GrameneiAT1G11910.1; AT1G11910.1; AT1G11910.
KEGGiath:AT1G11910.

Organism-specific databases

TAIRiAT1G11910.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiO65390.
KOiK08245.
OMAiKVERQVF.
OrthoDBiEOG093606Q0.
PhylomeDBiO65390.

Enzyme and pathway databases

BioCyciARA:AT1G11910-MONOMER.
ReactomeiR-ATH-2132295. MHC class II antigen presentation.

Miscellaneous databases

PROiO65390.

Gene expression databases

GenevisibleiO65390. AT.

Family and domain databases

Gene3Di1.10.225.10. 2 hits.
2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPA1_ARATH
AccessioniPrimary (citable) accession number: O65390
Secondary accession number(s): Q38934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.