Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartic proteinase A1

Gene

APA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the breakdown of propeptides of storage proteins in protein-storage vacuoles (By similarity). Possesses aspartic protease activity in vitro.By similarity1 Publication

pH dependencei

Optimum pH is 5.0-6.0 with insulin B chain as substrate and at 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100PROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • endopeptidase activity Source: TAIR

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • protein catabolic process Source: GO_Central
  • proteolysis Source: TAIR
  • response to cytokinin Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-ATH-2132295 MHC class II antigen presentation
R-ATH-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiA01.A02

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase A1 (EC:3.4.23.-)
Gene namesi
Name:APA1
Synonyms:PRA1
Ordered Locus Names:At1g11910
ORF Names:F12F1.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G11910
TAIRilocus:2008940 AT1G11910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000042062525 – 64Activation peptideSequence analysisAdd BLAST40
ChainiPRO_000042062665 – 506Aspartic proteinase A1Add BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi113 ↔ 119PROSITE-ProRule annotation
Disulfide bondi278 ↔ 282PROSITE-ProRule annotation
Disulfide bondi317 ↔ 411PROSITE-ProRule annotation
Disulfide bondi342 ↔ 383PROSITE-ProRule annotation
Disulfide bondi348 ↔ 380PROSITE-ProRule annotation
Glycosylationi397N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi425 ↔ 462PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO65390
PRIDEiO65390

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, petals, carpels, seed pods and dry seeds.1 Publication

Inductioni

By light and during senescence.2 Publications

Gene expression databases

ExpressionAtlasiO65390 baseline and differential
GenevisibleiO65390 AT

Interactioni

Protein-protein interaction databases

BioGridi22980, 12 interactors
IntActiO65390, 11 interactors
STRINGi3702.AT1G11910.1

Structurei

3D structure databases

ProteinModelPortaliO65390
SMRiO65390
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 503Peptidase A1PROSITE-ProRule annotationAdd BLAST422
Domaini312 – 417Saposin B-typePROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
HOGENOMiHOG000197681
InParanoidiO65390
KOiK08245
OMAiMAMDIPP
OrthoDBiEOG093606Q0
PhylomeDBiO65390

Family and domain databases

CDDicd06098 phytepsin, 1 hit
Gene3Di2.40.70.10, 1 hit
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
IPR033869 Phytepsin
IPR007856 SapB_1
IPR008138 SapB_2
IPR011001 Saposin-like
IPR008139 SaposinB_dom
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF00026 Asp, 1 hit
PF05184 SapB_1, 1 hit
PF03489 SapB_2, 1 hit
PRINTSiPR00792 PEPSIN
SMARTiView protein in SMART
SM00741 SapB, 1 hit
SUPFAMiSSF47862 SSF47862, 1 hit
SSF50630 SSF50630, 2 hits
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit
PS50015 SAP_B, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIYSRTVAV SLIVSFLLCF SAFAERNDGT FRVGLKKLKL DSKNRLAARV
60 70 80 90 100
ESKQEKPLRA YRLGDSGDAD VVVLKNYLDA QYYGEIAIGT PPQKFTVVFD
110 120 130 140 150
TGSSNLWVPS SKCYFSLACL LHPKYKSSRS STYEKNGKAA AIHYGTGAIA
160 170 180 190 200
GFFSNDAVTV GDLVVKDQEF IEATKEPGIT FVVAKFDGIL GLGFQEISVG
210 220 230 240 250
KAAPVWYNML KQGLIKEPVF SFWLNRNADE EEGGELVFGG VDPNHFKGKH
260 270 280 290 300
TYVPVTQKGY WQFDMGDVLI GGAPTGFCES GCSAIADSGT SLLAGPTTII
310 320 330 340 350
TMINHAIGAA GVVSQQCKTV VDQYGQTILD LLLSETQPKK ICSQIGLCTF
360 370 380 390 400
DGTRGVSMGI ESVVDKENAK LSNGVGDAAC SACEMAVVWI QSQLRQNMTQ
410 420 430 440 450
ERILNYVNEL CERLPSPMGE SAVDCAQLST MPTVSLTIGG KVFDLAPEEY
460 470 480 490 500
VLKVGEGPVA QCISGFIALD VAPPRGPLWI LGDVFMGKYH TVFDFGNEQV

GFAEAA
Length:506
Mass (Da):54,614
Last modified:August 1, 1998 - v1
Checksum:i171E16AA7A0D6FA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002131 Genomic DNA Translation: AAC17620.1
CP002684 Genomic DNA Translation: AEE28813.1
AY056387 mRNA Translation: AAL08243.1
AY056403 mRNA Translation: AAL08259.1
AY063974 mRNA Translation: AAL36330.1
BT001980 mRNA Translation: AAN71979.1
AY088657 mRNA Translation: AAM66979.1
U51036 mRNA Translation: AAC49730.1
PIRiF86253
RefSeqiNP_172655.1, NM_101062.4
UniGeneiAt.24544
At.67307

Genome annotation databases

EnsemblPlantsiAT1G11910.1; AT1G11910.1; AT1G11910
GeneIDi837740
GrameneiAT1G11910.1; AT1G11910.1; AT1G11910
KEGGiath:AT1G11910

Similar proteinsi

Entry informationi

Entry nameiAPA1_ARATH
AccessioniPrimary (citable) accession number: O65390
Secondary accession number(s): Q38934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health