O65388 (PLY2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative pectate lyase 2
EC=4.2.2.2

Gene names

Ordered Locus Names:	At1g11920
ORF Names:	F12F1.22

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.
Sequence caution	The sequence AAC17625.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 384	361	Putative pectate lyase 2		PRO_0000024866
Sites
	Active site	262	1	Potential
	Metal binding	182	1	Calcium By similarity
	Metal binding	206	1	Calcium By similarity
	Metal binding	210	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	O65388 [UniParc]. Last modified December 14, 2011. Version 2. Checksum: 0BA653FC08194A21	FASTA	384	42,693
	10 20 30 40 50 60 MASLFLTIIS LLFAAFSSSV VEAAYSNGYT IPKLLPNPID SCWRRNPYWA SNRRALADCA 70 80 90 100 110 120 VGFGKSAVGG KYGSIYVVTN PSDDPENPRP GTLRYAVIQS KPLWITFARD MVIVLRNELI 130 140 150 160 170 180 MNSYKTIDGR GAKVEIAYGP CITIQHVSHV IIHGISIHDC KPGKSGRVRS SPTHVGSRKG 190 200 210 220 230 240 SDGDAIAIFD SSHIWIDHCF FSRCQDGLID VLHASTAVTI SNNYFTQHDK VMLLGHNDNN 250 260 270 280 290 300 VEDKIMRVTI AFNHFGPGLI ERMPRVRRGY AHVANNRYEK WQMYAIGGSA DPTIFSEGNY 310 320 330 340 350 360 FVASDDPSKK QVTKRIDSGY DWKRWKWRTS KDVFKNGAYF VPSGYGTVTP LYGRAERFPV 370 380 SHGSLVPLLT SSAGPLHCYS GRIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002131 Genomic DNA. Translation: AAC17625.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE28815.1.
IPI	IPI00532903. IPI01020131.
PIR	H86253.
RefSeq	NP_172656.1. NM_101064.1.
UniGene	At.51578.
3D structure databases
ProteinModelPortal	O65388.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	O65388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	837742.
GenomeReviews	Gene locus AT1G11920 in contig CT485782_GR.
KEGG	ath:AT1G11920.
Organism-specific databases
TAIR	At1g11920.
Phylogenomic databases
eggNOG	euNOG10883.
GeneTree	EPGT00050000008990.
HOGENOM	HBG317381.
InParanoid	O65388.
PhylomeDB	O65388.
Gene expression databases
ArrayExpress	O65388.
Genevestigator	O65388.
GermOnline	AT1G11920. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY2_ARATH

Accession

Primary (citable) accession number: O65388
Secondary accession number(s): F4IAK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	December 14, 2011
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program