Reviewed, UniProtKB/Swiss-Prot O65388 (PEL2_ARATH)

Names and origin

Protein names

Recommended name:
    Putative pectate lyase 2
    EC=4.2.2.2

Gene names

Ordered Locus Names:	At1g11920
ORF Names:	F12F1.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 390	367	Putative pectate lyase 2		PRO_0000024866
Sites
	Active site	262	1	Potential
	Metal binding	182	1	Calcium By similarity
	Metal binding	206	1	Calcium By similarity
	Metal binding	210	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	O65388-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7E4D3CA9BB398CB7	FASTA	390	43,354
	10 20 30 40 50 60 MASLFLTIIS LLFAAFSSSV VEAAYSNGYT IPKLLPNPID SCWRRNPYWA SNRRALADCA 70 80 90 100 110 120 VGFGKSAVGG KYGSIYVVTN PSDDPENPRP GTLRYAVIQS KPLWITFARD MVIVLRNELI 130 140 150 160 170 180 MNSYKTIDGR GAKVEIAYGP CITIQHVSHV IIHGISIHDC KPGKSGRVRS SPTHVGSRKG 190 200 210 220 230 240 SDGDAIAIFD SSHIWIDHCF FSRCQDGLID VLHASTAVTI SNNYFTQHDK VMLLGHNDNN 250 260 270 280 290 300 VEDKIMRVTI AFNHFGPGLI ERMPRVRRGY AHVANNRYEK WQMYAIGGSA DPTIFSEGNY 310 320 330 340 350 360 FVASDDPSKK QVSSFKIVTK RIDSGYDWKR WKWRTSKDVF KNGAYFVPSG YGTVTPLYGR 370 380 390 AERFPVSHGS LVPLLTSSAG PLHCYSGRIC

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC002131 Genomic DNA. Translation: AAC17625.1.
IPI	IPI00532903.
PIR	H86253.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Genome annotation databases
GenomeReviews	Gene locus AT1G11920 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g11920.
Enzyme and pathway databases
BRENDA	4.2.2.2. 302.
Gene expression databases
ArrayExpress	O65388.
Genevestigator	O65388.
GermOnline	AT1G11920. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL2_ARATH

Accession

Primary (citable) accession number: O65388

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	August 1, 1998
Last modified:	October 13, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)